Wollprodukte im Wohnbereich : Untersuchungen zur Abschätzung des allergenen Potentials
The aim of this thesis is the assessment of the allergenic potential towards wool products from indoor living areas (e.g. carpets, bedding and insulating material) with skin contact and with contact by the respiratory tract for inhale particles. New and used wool products were characterised with protein chemical methods (amino acid analysis and SDS gel electrophoresis). The processing agents (e.g. flame retarding and mothproofing agents) and substances like pesticides were characterised with analytic methods. The long-term use of wool products from indoor living areas can lead to changes of the products. Mechanical influence is important by carpets and is leading to damages on the surface of the carpet and the wool fibres. Fibrillates wool fibres were found. Thermal influence is important by insulating material and beddings and is leading only to small changes on the wool fibre surface. By insulating material humidity could not provoke the building of mildew. Mite contamination was founded by several beddings. This mite contamination must be involved by the investigation of the allergenic potential. The changes of the wool products generated by thermal influences are be simulated by an artificial thermal ageing process. The wool before and after ageing is not significantly different in the composition of the amino acids. But it was found a significantly decrease in the band pattern in the area of the KIF. This decrease was caused by new protein cross linkage (lanthionine and isodipeptide linkage (epsilon-(gamma-glutamyl)lysine). The changes of the processing agents are characterised by analytical methods. During the long-term use of the products dust particles can be produced. The building of dust was simulated by laboratory tests for mechanical weakly influenced products (e.g. insulating material, bedding) and mechanical strongly influenced products (e.g. carpets). Wool dust is a complex mixture of the wool, inorganic and organic compounds from the processing agents and substances of the environment. The dust particles of wool fibres are wool fibre fragments of different dimension, cortical cells, cortical fragments, polygonal cuticular cells and cuticular cell fragments. The total airborne dust and the fine airborne dust of a carpet were collected with a dust sampler. The collected dust was characterised. The investigation of the solubility of wool and wool dust was made with physiological model solution under in-vitro-conditions. The model solution of sweat and bronchial fluid are modified with enzymes for simulate the enzymatic activity of human fluids. The incubation of beddings with synthetic enzyme free and modified sweat solution solved only a small amount of proteins (max. 1%). The soluble proteins show a great similarity to the PCL of the wool and the endocuticula. The incubation of dust of insulating materials and carpets with enzyme free and modified solution for the extracellular and intracellular lung solution solved only a small amount of proteins (max. 2%). The soluble proteins show a substantial similarity to the PCL and the endocuticula and by the use of the enzyme trypsine to the proteins of the medulla and of the intermediarfilaments. However neither wool nor wool dust could be solved by the model solution under the conditions of these investigations. The dermatological tests (prick test) of extracts from the wool products showed no significantly reactions by test persons with a positive wool intolerance. Processing agents and the ageing of the products showed likewise no influences. No type-I-allergy is released by the solved proteins and substances from the wool products.
info:eu-repo/classification/ddc/610, Medizin, Wolle, Dämmwolle, Bettwaren, Teppiche, proteinchemische Charakterisierung, Mottenschutz, Flammschutz, Alterung, künstliche Alterung, Isodipeptidbindung, Wollstaub, E-Staub, A-Staub, Humanschweißmodelle, Modelllungenlösungen, Enzymmodifizierung
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