Thermostabile und alkaliresistente Proteasen in der Wollveredlung : Möglichkeiten der industriellen Nutzung und Auswirkungen auf Wolle
type: CreativeWork
description: This work aimed at the industry related development of an enzymatic finishing treatment based on the finishing processes of a medium-seized textile plant (Tuchwerk Westmark, Mönchengladbach (TW)). Moreover the wool modifying potential of enzyme preparations from newly isolated hyperthermophilic micro organisms provided by the TU Hamburg-Harburg (TU-HH) were to be investigated. Of the three enzyme preparations from the TU-HH only the culture supernatants of Fervidobacterium gondwanense and Thermoanaerobacter keratinophilus are generally able to modify wool. However due to the low activity the industrial use of the culture supernatant of F. gondwanense in reasonable treatment time and dosage is not sensible. The enzyme fraction of the culture supernatant of T. keratinophilus showed a higher activity towards wool. Nonetheless the incubation didn’t result in positive effects on the degree of whiteness or the dye ability of wool top. The available portion of enzyme only allowed a limited amount of experiments. Therefore a concluding evaluation of a potential use of the enzyme preparation of T. keratinophilus in the textile technical process was not possible. Investigations in laboratory scale with the commercial available thermo stable and alkali-resistant serine protease Esperase 8.0 L (Novo Nordisk) showed that the treatment parameters of the pre washing step to the dyeing of wool fabrics at TW are suitable for the integration of Esperase 8.0 L. The hard well water used at TW and the added water softener showed no impact on the enzyme catalysed wool modification. The auxiliaries used have synergistic effects on the influence of the enzyme with regard to the degree of whiteness and the dye ability. After the completed pre washing the Esperase 8.0 L does not show an activity, it is consequently irreversible heat-denatured. The denaturation is necessary to rule out uncontrolled enzyme catalysed reactions in the following finishing steps or at the end consumer (e.g. while …
abstract: This work aimed at the industry related development of an enzymatic finishing treatment based on the finishing processes of a medium-seized textile plant (Tuchwerk Westmark, Mönchengladbach (TW)). Moreover the wool modifying potential of enzyme preparations from newly isolated hyperthermophilic micro organisms provided by the TU Hamburg-Harburg (TU-HH) were to be investigated. Of the three enzyme preparations from the TU-HH only the culture supernatants of Fervidobacterium gondwanense and Thermoanaerobacter keratinophilus are generally able to modify wool. However due to the low activity the industrial use of the culture supernatant of F. gondwanense in reasonable treatment time and dosage is not sensible. The enzyme fraction of the culture supernatant of T. keratinophilus showed a higher activity towards wool. Nonetheless the incubation didn’t result in positive effects on the degree of whiteness or the dye ability of wool top. The available portion of enzyme only allowed a limited amount of experiments. Therefore a concluding evaluation of a potential use of the enzyme preparation of T. keratinophilus in the textile technical process was not possible. Investigations in laboratory scale with the commercial available thermo stable and alkali-resistant serine protease Esperase 8.0 L (Novo Nordisk) showed that the treatment parameters of the pre washing step to the dyeing of wool fabrics at TW are suitable for the integration of Esperase 8.0 L. The hard well water used at TW and the added water softener showed no impact on the enzyme catalysed wool modification. The auxiliaries used have synergistic effects on the influence of the enzyme with regard to the degree of whiteness and the dye ability. After the completed pre washing the Esperase 8.0 L does not show an activity, it is consequently irreversible heat-denatured. The denaturation is necessary to rule out uncontrolled enzyme catalysed reactions in the following finishing steps or at the end consumer (e.g. while …
author: Schumacher, Karin
language: ger
keywords: info:eu-repo/classification/ddc/660, Technische Chemie, Wolle, enzymatische Veredlung, thermostabile und alkaliresistente Proteasen
date published: 2002
date modified: 2026-07-30T19:42:39Z
provider: RWTH Aachen University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Publikationsserver der RWTH Aachen University