{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:59534"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:59534","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Analysen zur Funktion von Mehmet, einem neuen Interaktionspartner des Transkriptionsfaktors AP-2-alpha, sowie Charakterisierung von Mehmet in Drosophila melanogaster","abstract":"AP-2 transcription factors represent a family of five closely related and evolutionarily conserved DNA-binding proteins termed AP-2alpha, AP-2beta, AP-2gamma, AP-2delta and AP-2epsilon. They are characterized by an unique modular structure consisting of an aminoterminal transcriptional activation domain and a complex helix span helix motif for dimerization and DNA binding. AP-2 proteins have been suggested to regulate a variety of target genes, embryonic development, apoptosis and differentiation. Here we report the characterization of Mehmet, a potential new AP-2 interacting protein, isolated by Far Western screening. The cDNA encodes a 1306 aa protein with a molecular mass of apparently 150 kD. Sequence analysis revealed 3 main domains: The N-terminal region shows homology to different yeast-proteins (Y-Box), followed by an acidic region. Homology with 5‘ cytosine DNA methyltransferase and Drosophila trithorax protein was found in the central section, causing the name Mehmet (protein containing MECP-1 homology region similar to DNA-methyltransferase and mammalian trithorax proteins). The C-terminal domain is homologue to F-box- and leucin motives. Expression analysis revealed a 4.4 kb Mehmet transcript in liver and kidney, correlating to the expression pattern of AP-2alpha, and expression was also shown in brain, testis, spleen and heart. To confirm interaction between AP-2 and Mehmet in vitro and in vivo we performed GST pull down ­­­­­­­­­­­– and IP experiments. AP-2alpha, AP-2beta and AP-2gamma were tested and showed a positive result in GST-pull down assay used for the confirmation of AP-2/Mehmet interaction. Immunofluorescence microscopy revealed a translocation of Mehmet within the cell from cytoplasm into the nucleus. To understand the function we established stable transfectants with inducibly Mehmet expression. Potential degradation of AP-2alpha by Mehmet was researched and constant level of AP-2alpha in Western-Blot analysis proved that Mehmet has no influence on the stability of AP-2alpha. Proliferation and apoptosis assays demonstrated no functional interference for inducible expression of Mehmet. Data search analysis identified a highly conserved Mehmet-gene in Drosophila melanogaster (dMehmet). Expression Analysis of dMehmet by Northern Blot, RT-PCR and in situ hybridisation confirm a persistent expression pattern of dMehmet during Drosophila development.","abstract_html":"AP-2 transcription factors represent a family of five closely related and evolutionarily conserved DNA-binding proteins termed AP-2alpha, AP-2beta, AP-2gamma, AP-2delta and AP-2epsilon. They are characterized by an unique modular structure consisting of an aminoterminal transcriptional activation domain and a complex helix span helix motif for dimerization and DNA binding. AP-2 proteins have been suggested to regulate a variety of target genes, embryonic development, apoptosis and differentiation. Here we report the characterization of Mehmet, a potential new AP-2 interacting protein, isolated by Far Western screening. The cDNA encodes a 1306 aa protein with a molecular mass of apparently 150 kD. Sequence analysis revealed 3 main domains: The N-terminal region shows homology to different yeast-proteins (Y-Box), followed by an acidic region. Homology with 5‘ cytosine DNA methyltransferase and Drosophila trithorax protein was found in the central section, causing the name Mehmet (protein containing MECP-1 homology region similar to DNA-methyltransferase and mammalian trithorax proteins). The C-terminal domain is homologue to F-box- and leucin motives. Expression analysis revealed a 4.4 kb Mehmet transcript in liver and kidney, correlating to the expression pattern of AP-2alpha, and expression was also shown in brain, testis, spleen and heart. To confirm interaction between AP-2 and Mehmet in vitro and in vivo we performed GST pull down ­­­­­­­­­­­– and IP experiments. AP-2alpha, AP-2beta and AP-2gamma were tested and showed a positive result in GST-pull down assay used for the confirmation of AP-2/Mehmet interaction. Immunofluorescence microscopy revealed a translocation of Mehmet within the cell from cytoplasm into the nucleus. To understand the function we established stable transfectants with inducibly Mehmet expression. Potential degradation of AP-2alpha by Mehmet was researched and constant level of AP-2alpha in Western-Blot analysis proved that Mehmet has no influence on the stability of AP-2alpha. Proliferation and apoptosis assays demonstrated no functional interference for inducible expression of Mehmet. Data search analysis identified a highly conserved Mehmet-gene in Drosophila melanogaster (dMehmet). Expression Analysis of dMehmet by Northern Blot, RT-PCR and in situ hybridisation confirm a persistent expression pattern of dMehmet during Drosophila development.","abstract_has_math":false,"creators":["Septinus, Anja"],"institution":"Publikationsserver der RWTH Aachen University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Büttner, Reinhard"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2004,"date_issued":"2004","date_published":"2004","updated_at":"2026-07-30T19:42:39Z","subjects":["info:eu-repo/classification/ddc/570","Taufliege","Transkriptionsfaktor","cDNS","Wechselwirkung","Biowissenschaften, Biologie","Mehmet","Transkriptionsfaktor AP-2alpha","Drosophila melanogaster"],"languages":["ger"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121313%22"],"render_values":[{"text":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121313%22","href":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121313%22","code":true}]}]},"links":{"outbound_url":"https://publications.rwth-aachen.de/record/59534","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Büttner, Reinhard"]},{"key":"dc:creator","label":"Author","values":["Septinus, Anja"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:coverage","label":"Dc Coverage","values":["DE"]},{"key":"dc:date","label":"Dc Date","values":["2004"]},{"key":"dc:publisher","label":"Institution","values":["Publikationsserver der RWTH Aachen University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-8914"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["info:eu-repo/classification/ddc/570","Taufliege","Transkriptionsfaktor","cDNS","Wechselwirkung","Biowissenschaften, Biologie","Mehmet","Transkriptionsfaktor AP-2alpha","Drosophila melanogaster"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ger"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/record/59534","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121313%22"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["AP-2 transcription factors represent a family of five closely related and evolutionarily conserved DNA-binding proteins termed AP-2alpha, AP-2beta, AP-2gamma, AP-2delta and AP-2epsilon. They are characterized by an unique modular structure consisting of an aminoterminal transcriptional activation domain and a complex helix span helix motif for dimerization and DNA binding. AP-2 proteins have been suggested to regulate a variety of target genes, embryonic development, apoptosis and differentiation. Here we report the characterization of Mehmet, a potential new AP-2 interacting protein, isolated by Far Western screening. The cDNA encodes a 1306 aa protein with a molecular mass of apparently 150 kD. Sequence analysis revealed 3 main domains: The N-terminal region shows homology to different yeast-proteins (Y-Box), followed by an acidic region. Homology with 5‘ cytosine DNA methyltransferase and Drosophila trithorax protein was found in the central section, causing the name Mehmet (protein containing MECP-1 homology region similar to DNA-methyltransferase and mammalian trithorax proteins). The C-terminal domain is homologue to F-box- and leucin motives. Expression analysis revealed a 4.4 kb Mehmet transcript in liver and kidney, correlating to the expression pattern of AP-2alpha, and expression was also shown in brain, testis, spleen and heart. To confirm interaction between AP-2 and Mehmet in vitro and in vivo we performed GST pull down ­­­­­­­­­­­– and IP experiments. AP-2alpha, AP-2beta and AP-2gamma were tested and showed a positive result in GST-pull down assay used for the confirmation of AP-2/Mehmet interaction. Immunofluorescence microscopy revealed a translocation of Mehmet within the cell from cytoplasm into the nucleus. To understand the function we established stable transfectants with inducibly Mehmet expression. Potential degradation of AP-2alpha by Mehmet was researched and constant level of AP-2alpha in Western-Blot analysis proved that Mehmet has no influence on the stability of AP-2alpha. Proliferation and apoptosis assays demonstrated no functional interference for inducible expression of Mehmet. Data search analysis identified a highly conserved Mehmet-gene in Drosophila melanogaster (dMehmet). Expression Analysis of dMehmet by Northern Blot, RT-PCR and in situ hybridisation confirm a persistent expression pattern of dMehmet during Drosophila development."]},{"key":"dc:source","label":"Dc Source","values":["Aachen : Publikationsserver der RWTH Aachen University 161 S. : Ill., graph. Darst. (2004). = Aachen, Techn. Hochsch., Diss., 2004"]},{"key":"dc:title","label":"Title","values":["Analysen zur Funktion von Mehmet, einem neuen Interaktionspartner des Transkriptionsfaktors AP-2-alpha, sowie Charakterisierung von Mehmet in Drosophila melanogaster"]}]}],"canonical_facts":{"dc:contributor":["Büttner, Reinhard"],"dc:coverage":["DE"],"dc:creator":["Septinus, Anja"],"dc:date":["2004"],"dc:description":["AP-2 transcription factors represent a family of five closely related and evolutionarily conserved DNA-binding proteins termed AP-2alpha, AP-2beta, AP-2gamma, AP-2delta and AP-2epsilon. They are characterized by an unique modular structure consisting of an aminoterminal transcriptional activation domain and a complex helix span helix motif for dimerization and DNA binding. AP-2 proteins have been suggested to regulate a variety of target genes, embryonic development, apoptosis and differentiation. Here we report the characterization of Mehmet, a potential new AP-2 interacting protein, isolated by Far Western screening. The cDNA encodes a 1306 aa protein with a molecular mass of apparently 150 kD. Sequence analysis revealed 3 main domains: The N-terminal region shows homology to different yeast-proteins (Y-Box), followed by an acidic region. Homology with 5‘ cytosine DNA methyltransferase and Drosophila trithorax protein was found in the central section, causing the name Mehmet (protein containing MECP-1 homology region similar to DNA-methyltransferase and mammalian trithorax proteins). The C-terminal domain is homologue to F-box- and leucin motives. Expression analysis revealed a 4.4 kb Mehmet transcript in liver and kidney, correlating to the expression pattern of AP-2alpha, and expression was also shown in brain, testis, spleen and heart. To confirm interaction between AP-2 and Mehmet in vitro and in vivo we performed GST pull down ­­­­­­­­­­­– and IP experiments. AP-2alpha, AP-2beta and AP-2gamma were tested and showed a positive result in GST-pull down assay used for the confirmation of AP-2/Mehmet interaction. Immunofluorescence microscopy revealed a translocation of Mehmet within the cell from cytoplasm into the nucleus. To understand the function we established stable transfectants with inducibly Mehmet expression. Potential degradation of AP-2alpha by Mehmet was researched and constant level of AP-2alpha in Western-Blot analysis proved that Mehmet has no influence on the stability of AP-2alpha. Proliferation and apoptosis assays demonstrated no functional interference for inducible expression of Mehmet. Data search analysis identified a highly conserved Mehmet-gene in Drosophila melanogaster (dMehmet). Expression Analysis of dMehmet by Northern Blot, RT-PCR and in situ hybridisation confirm a persistent expression pattern of dMehmet during Drosophila development."],"dc:identifier":["https://publications.rwth-aachen.de/record/59534","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121313%22"],"dc:language":["ger"],"dc:publisher":["Publikationsserver der RWTH Aachen University"],"dc:relation":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-8914"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:source":["Aachen : Publikationsserver der RWTH Aachen University 161 S. : Ill., graph. Darst. (2004). = Aachen, Techn. Hochsch., Diss., 2004"],"dc:subject":["info:eu-repo/classification/ddc/570","Taufliege","Transkriptionsfaktor","cDNS","Wechselwirkung","Biowissenschaften, Biologie","Mehmet","Transkriptionsfaktor AP-2alpha","Drosophila melanogaster"],"dc:title":["Analysen zur Funktion von Mehmet, einem neuen Interaktionspartner des Transkriptionsfaktors AP-2-alpha, sowie Charakterisierung von Mehmet in Drosophila melanogaster"],"dc:type":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]},"updated_at":"2026-07-30T19:42:39Z"}