Identifizierung differentiell exprimierter Gene nach zentraler und peripherer Nervenläsion mittels differential display PCR(ddPCR)
Background: It is well known that neurons of the peripheral nervous system do have the capacity to regenerate a severed axon leading to functional recovery, whereas neurons of the central nervous system do not regenerate successfully after injury. The underlying molecular programs initiated by axotomized peripheral and central nervous system neurons are not yet fully understood. Results: To gain insight into the molecular mechanisms underlying the process of regeneration in the nervous system, differential display polymerase chain reaction has been used to identify differntially expressed genes following axotomy of peripheral and central nerve fibers. For this purpose, axotomy induced changes of regenerating facial nucleus neurons and non-regenerating red nucleus and Clarke ‘s nucleus neurons have been analyzed in an intra-animal side-to-side comparison. One hundred and nine gene fragments have been isolated, of which 37 correspond to known genes encoding for a number of different functional classes of proteins such as transcription factors, signaling molecules, homeobox-genes, receptors and proteins involved in metabolism. Seventy-two gene-fragments did not show similarities to known genes. In this study differential display PCR was used as a screening method to analyze differntially expressed genes. Furthermore the method itself was established and critically assessed in terms of sensitivity and specificity.
info:eu-repo/classification/ddc/610, Medizin, ddPCR, Ncl.Ruber, differential display PCR
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