{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:57216"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:57216","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Transport von Paraaminohippursäure, Furosemid, Hydrochlorothiazid, Cimetidin, 11-Deoxycorticosteron und Taurocholat an der basolateralen Membran proximaler Nierentubuli","abstract":"The renal basolateral transport system is an efficient system for secretion of a wide range of different drugs. One important part of this system is the organic anion transport system (OATS1), which is known for its wide range of substrate-specifity. Previous studies could demonstrate the interaction of a variety of substrates with OATS1, but could not demonstrate the translocation of those substrates into the tubular cell. Therefore, in the present study, the basolateral transport rate of different substrates (p-aminohippurate (PAH), furosemide, hydrochlorothiazide (HCT), cimetidine, 11-deoxycorticosteron and taurocholate) was determined on non-perfused proximal S2-Segments, microdissected from rabbit kidneys without use of enzymatic agents. To measure the transport rate, we took advantage of the finding, that OATS1 acts as a counter-transporter, which exchanges PAH (the classical substrate for this transport system) and other drugs with dicarboxylates (glutarate or a-ketoglutarate). Hence, the stimulatory effect of a drug on the 14C-glutarate efflux-rate of 14C-glutarate preloaded S2-segments is a measure of its translocation across the basolateral cell membrane. The efflux rate of 14C-glutarate was determined in presence of the investigated drugs by comparing the accumulation ratio in the tubular cells in presence of the drug and with control conditions. In presence of PAH (1000 µmol) and furosemide (400 µmol), a significantly (p=0,032 and p=0.0838) efflux-rate of 14C-glutarate could be demonstrated. These findings indicate, that PAH and furosemide are transported by the renal basolateral transport system for organic anions, just as Taurocholate (1000 µmol), which slightly stimulated the efflux of 14C-glutarate. Hydrochlorothiazide (1400 µmol), Cimetidine (98 µmol) and 11-Deoxycorticosteron (70 µmol) did not stimulate the 14C-glutarate efflux. It is concluded, that HCT, cimetidine and 11-deoxycorticosteron are not translocated by the renal basolateral transport system for organic anions, but from another transport system of the kidney.","abstract_html":"The renal basolateral transport system is an efficient system for secretion of a wide range of different drugs. One important part of this system is the organic anion transport system (OATS1), which is known for its wide range of substrate-specifity. Previous studies could demonstrate the interaction of a variety of substrates with OATS1, but could not demonstrate the translocation of those substrates into the tubular cell. Therefore, in the present study, the basolateral transport rate of different substrates (p-aminohippurate (PAH), furosemide, hydrochlorothiazide (HCT), cimetidine, 11-deoxycorticosteron and taurocholate) was determined on non-perfused proximal S2-Segments, microdissected from rabbit kidneys without use of enzymatic agents. To measure the transport rate, we took advantage of the finding, that OATS1 acts as a counter-transporter, which exchanges PAH (the classical substrate for this transport system) and other drugs with dicarboxylates (glutarate or a-ketoglutarate). Hence, the stimulatory effect of a drug on the 14C-glutarate efflux-rate of 14C-glutarate preloaded S2-segments is a measure of its translocation across the basolateral cell membrane. The efflux rate of 14C-glutarate was determined in presence of the investigated drugs by comparing the accumulation ratio in the tubular cells in presence of the drug and with control conditions. In presence of PAH (1000 µmol) and furosemide (400 µmol), a significantly (p=0,032 and p=0.0838) efflux-rate of 14C-glutarate could be demonstrated. These findings indicate, that PAH and furosemide are transported by the renal basolateral transport system for organic anions, just as Taurocholate (1000 µmol), which slightly stimulated the efflux of 14C-glutarate. Hydrochlorothiazide (1400 µmol), Cimetidine (98 µmol) and 11-Deoxycorticosteron (70 µmol) did not stimulate the 14C-glutarate efflux. It is concluded, that HCT, cimetidine and 11-deoxycorticosteron are not translocated by the renal basolateral transport system for organic anions, but from another transport system of the kidney.","abstract_has_math":false,"creators":["Haßler, Martin Alexander"],"institution":"Publikationsserver der RWTH Aachen University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Greven, Joachim","Heintz, Joachim"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2002,"date_issued":"2002","date_published":"2002","updated_at":"2026-07-30T19:42:09Z","subjects":["info:eu-repo/classification/ddc/610","Medizin"],"languages":["ger"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-119274%22"],"render_values":[{"text":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-119274%22","href":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-119274%22","code":true}]}]},"links":{"outbound_url":"https://publications.rwth-aachen.de/record/57216","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Greven, Joachim","Heintz, Joachim"]},{"key":"dc:creator","label":"Author","values":["Haßler, Martin Alexander"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:coverage","label":"Dc Coverage","values":["DE"]},{"key":"dc:date","label":"Dc Date","values":["2002"]},{"key":"dc:publisher","label":"Institution","values":["Publikationsserver der RWTH Aachen University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-4690"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["info:eu-repo/classification/ddc/610","Medizin"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ger"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/record/57216","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-119274%22"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The renal basolateral transport system is an efficient system for secretion of a wide range of different drugs. One important part of this system is the organic anion transport system (OATS1), which is known for its wide range of substrate-specifity. Previous studies could demonstrate the interaction of a variety of substrates with OATS1, but could not demonstrate the translocation of those substrates into the tubular cell. Therefore, in the present study, the basolateral transport rate of different substrates (p-aminohippurate (PAH), furosemide, hydrochlorothiazide (HCT), cimetidine, 11-deoxycorticosteron and taurocholate) was determined on non-perfused proximal S2-Segments, microdissected from rabbit kidneys without use of enzymatic agents. To measure the transport rate, we took advantage of the finding, that OATS1 acts as a counter-transporter, which exchanges PAH (the classical substrate for this transport system) and other drugs with dicarboxylates (glutarate or a-ketoglutarate). Hence, the stimulatory effect of a drug on the 14C-glutarate efflux-rate of 14C-glutarate preloaded S2-segments is a measure of its translocation across the basolateral cell membrane. The efflux rate of 14C-glutarate was determined in presence of the investigated drugs by comparing the accumulation ratio in the tubular cells in presence of the drug and with control conditions. In presence of PAH (1000 µmol) and furosemide (400 µmol), a significantly (p=0,032 and p=0.0838) efflux-rate of 14C-glutarate could be demonstrated. These findings indicate, that PAH and furosemide are transported by the renal basolateral transport system for organic anions, just as Taurocholate (1000 µmol), which slightly stimulated the efflux of 14C-glutarate. Hydrochlorothiazide (1400 µmol), Cimetidine (98 µmol) and 11-Deoxycorticosteron (70 µmol) did not stimulate the 14C-glutarate efflux. It is concluded, that HCT, cimetidine and 11-deoxycorticosteron are not translocated by the renal basolateral transport system for organic anions, but from another transport system of the kidney."]},{"key":"dc:source","label":"Dc Source","values":["Aachen : Publikationsserver der RWTH Aachen University 80 S. : Ill., graph. Darst. (2002). = Aachen, Techn. Hochsch., Diss., 2002"]},{"key":"dc:title","label":"Title","values":["Transport von Paraaminohippursäure, Furosemid, Hydrochlorothiazid, Cimetidin, 11-Deoxycorticosteron und Taurocholat an der basolateralen Membran proximaler Nierentubuli"]}]}],"canonical_facts":{"dc:contributor":["Greven, Joachim","Heintz, Joachim"],"dc:coverage":["DE"],"dc:creator":["Haßler, Martin Alexander"],"dc:date":["2002"],"dc:description":["The renal basolateral transport system is an efficient system for secretion of a wide range of different drugs. One important part of this system is the organic anion transport system (OATS1), which is known for its wide range of substrate-specifity. Previous studies could demonstrate the interaction of a variety of substrates with OATS1, but could not demonstrate the translocation of those substrates into the tubular cell. Therefore, in the present study, the basolateral transport rate of different substrates (p-aminohippurate (PAH), furosemide, hydrochlorothiazide (HCT), cimetidine, 11-deoxycorticosteron and taurocholate) was determined on non-perfused proximal S2-Segments, microdissected from rabbit kidneys without use of enzymatic agents. To measure the transport rate, we took advantage of the finding, that OATS1 acts as a counter-transporter, which exchanges PAH (the classical substrate for this transport system) and other drugs with dicarboxylates (glutarate or a-ketoglutarate). Hence, the stimulatory effect of a drug on the 14C-glutarate efflux-rate of 14C-glutarate preloaded S2-segments is a measure of its translocation across the basolateral cell membrane. The efflux rate of 14C-glutarate was determined in presence of the investigated drugs by comparing the accumulation ratio in the tubular cells in presence of the drug and with control conditions. In presence of PAH (1000 µmol) and furosemide (400 µmol), a significantly (p=0,032 and p=0.0838) efflux-rate of 14C-glutarate could be demonstrated. These findings indicate, that PAH and furosemide are transported by the renal basolateral transport system for organic anions, just as Taurocholate (1000 µmol), which slightly stimulated the efflux of 14C-glutarate. Hydrochlorothiazide (1400 µmol), Cimetidine (98 µmol) and 11-Deoxycorticosteron (70 µmol) did not stimulate the 14C-glutarate efflux. It is concluded, that HCT, cimetidine and 11-deoxycorticosteron are not translocated by the renal basolateral transport system for organic anions, but from another transport system of the kidney."],"dc:identifier":["https://publications.rwth-aachen.de/record/57216","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-119274%22"],"dc:language":["ger"],"dc:publisher":["Publikationsserver der RWTH Aachen University"],"dc:relation":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-4690"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:source":["Aachen : Publikationsserver der RWTH Aachen University 80 S. : Ill., graph. Darst. (2002). = Aachen, Techn. Hochsch., Diss., 2002"],"dc:subject":["info:eu-repo/classification/ddc/610","Medizin"],"dc:title":["Transport von Paraaminohippursäure, Furosemid, Hydrochlorothiazid, Cimetidin, 11-Deoxycorticosteron und Taurocholat an der basolateralen Membran proximaler Nierentubuli"],"dc:type":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]},"updated_at":"2026-07-30T19:42:09Z"}