{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:57215"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:57215","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Untersuchung zu Veränderungen der Lymphozytensubpopulationen im Krankheitsverlauf der Mukoviszidose","abstract":"Lung disease in cystic fibrosis (CF) is characterized by intrabronchial and interstitial inflammation. Compared with the intrabronchial compartment, knowledge on the lymphocyte system and on interstitial pneumonia in CF is limited. As changes of peripheral blood lymphocyte subsets (LS) may accompany severe systemic lymphocyte immune responses, we compared peripheral LS of 44 patients with CF, 23 non-CF Patients with recurrent pulmonary infections and 83 healthy controls. We performed lymphocyte phenotyping with CD3-, CD19-, CD16-, CD56-, CD4-, CD8-, CD11b-, CD57-, HLA-DR-, CD25-, CD45RA-, CD45RO- antigens. Additional immunohistochemistry was performed on lung tissue of four CF patients aged 0.5, 12, 17 and 20 years. We analyzed age depending and age independent differences between the three mentioned groups. Additionally we investigated the age depending changes within the patients with CF and the healthy controls. Comparison of patients and controls showed a marked lowering of CD4(+)CD45RO(+)-memory-cells, of T cells expressing IL-2R, of CD8(+) cells and of CD 16(+)56(+)-cells. Compared to healthy controls patients with recurrent broonchopulmonary infections showed similar changes. Immunohistochemistry showed activation of bronchus-associated lymphoid tissue with interstitial accumulation of CD4(+)CD45RO(+)-T-cells in the three older patients. This investigation is the first to demonstrate marked changes of peripheral lymphocyte subsets in patients with CF. As shown by the group of non-CF patients with pulmonary infections the registered changes of the lymphocyte subsets are not specific for CF. Furthermore the mentioned pulmonary homing of CD4(+)CD45RO(+)-cells seems to be physiologically. We presume that the interstitial accumulation of lymphocytes in lungs of patients with CF, which leads to a peripheral decrease of these cells, is increased. It is possible, that the pulmonary homing of CD4(+)CD45(+)R0-cells is a part of the pathophysiology of the lung disease in patients with CF. An immune response dominated by CD4(+)-cells may contribute to the persistence of the pulmonary inflammation. Possibly, immunophenotyping of peripheral blood LS will become a useful indirect tool for assessment of interstitial pulmonary inflammation in CF.","abstract_html":"Lung disease in cystic fibrosis (CF) is characterized by intrabronchial and interstitial inflammation. Compared with the intrabronchial compartment, knowledge on the lymphocyte system and on interstitial pneumonia in CF is limited. As changes of peripheral blood lymphocyte subsets (LS) may accompany severe systemic lymphocyte immune responses, we compared peripheral LS of 44 patients with CF, 23 non-CF Patients with recurrent pulmonary infections and 83 healthy controls. We performed lymphocyte phenotyping with CD3-, CD19-, CD16-, CD56-, CD4-, CD8-, CD11b-, CD57-, HLA-DR-, CD25-, CD45RA-, CD45RO- antigens. Additional immunohistochemistry was performed on lung tissue of four CF patients aged 0.5, 12, 17 and 20 years. We analyzed age depending and age independent differences between the three mentioned groups. Additionally we investigated the age depending changes within the patients with CF and the healthy controls. Comparison of patients and controls showed a marked lowering of CD4(+)CD45RO(+)-memory-cells, of T cells expressing IL-2R, of CD8(+) cells and of CD 16(+)56(+)-cells. Compared to healthy controls patients with recurrent broonchopulmonary infections showed similar changes. Immunohistochemistry showed activation of bronchus-associated lymphoid tissue with interstitial accumulation of CD4(+)CD45RO(+)-T-cells in the three older patients. This investigation is the first to demonstrate marked changes of peripheral lymphocyte subsets in patients with CF. As shown by the group of non-CF patients with pulmonary infections the registered changes of the lymphocyte subsets are not specific for CF. Furthermore the mentioned pulmonary homing of CD4(+)CD45RO(+)-cells seems to be physiologically. We presume that the interstitial accumulation of lymphocytes in lungs of patients with CF, which leads to a peripheral decrease of these cells, is increased. It is possible, that the pulmonary homing of CD4(+)CD45(+)R0-cells is a part of the pathophysiology of the lung disease in patients with CF. An immune response dominated by CD4(+)-cells may contribute to the persistence of the pulmonary inflammation. Possibly, immunophenotyping of peripheral blood LS will become a useful indirect tool for assessment of interstitial pulmonary inflammation in CF.","abstract_has_math":false,"creators":["Opladen, Thomas"],"institution":"Publikationsserver der RWTH Aachen University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Heimann, Gerhard"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2002,"date_issued":"2002","date_published":"2002","updated_at":"2026-07-30T19:42:09Z","subjects":["info:eu-repo/classification/ddc/610","Medizin","Lymphozytensubpopulationen","Mukoviszidose"],"languages":["ger"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-119273%22"],"render_values":[{"text":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-119273%22","href":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-119273%22","code":true}]}]},"links":{"outbound_url":"https://publications.rwth-aachen.de/record/57215","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Heimann, Gerhard"]},{"key":"dc:creator","label":"Author","values":["Opladen, Thomas"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:coverage","label":"Dc Coverage","values":["DE"]},{"key":"dc:date","label":"Dc Date","values":["2002"]},{"key":"dc:publisher","label":"Institution","values":["Publikationsserver der RWTH Aachen University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-4782"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["info:eu-repo/classification/ddc/610","Medizin","Lymphozytensubpopulationen","Mukoviszidose"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ger"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/record/57215","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-119273%22"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Lung disease in cystic fibrosis (CF) is characterized by intrabronchial and interstitial inflammation. Compared with the intrabronchial compartment, knowledge on the lymphocyte system and on interstitial pneumonia in CF is limited. As changes of peripheral blood lymphocyte subsets (LS) may accompany severe systemic lymphocyte immune responses, we compared peripheral LS of 44 patients with CF, 23 non-CF Patients with recurrent pulmonary infections and 83 healthy controls. We performed lymphocyte phenotyping with CD3-, CD19-, CD16-, CD56-, CD4-, CD8-, CD11b-, CD57-, HLA-DR-, CD25-, CD45RA-, CD45RO- antigens. Additional immunohistochemistry was performed on lung tissue of four CF patients aged 0.5, 12, 17 and 20 years. We analyzed age depending and age independent differences between the three mentioned groups. Additionally we investigated the age depending changes within the patients with CF and the healthy controls. Comparison of patients and controls showed a marked lowering of CD4(+)CD45RO(+)-memory-cells, of T cells expressing IL-2R, of CD8(+) cells and of CD 16(+)56(+)-cells. Compared to healthy controls patients with recurrent broonchopulmonary infections showed similar changes. Immunohistochemistry showed activation of bronchus-associated lymphoid tissue with interstitial accumulation of CD4(+)CD45RO(+)-T-cells in the three older patients. This investigation is the first to demonstrate marked changes of peripheral lymphocyte subsets in patients with CF. As shown by the group of non-CF patients with pulmonary infections the registered changes of the lymphocyte subsets are not specific for CF. Furthermore the mentioned pulmonary homing of CD4(+)CD45RO(+)-cells seems to be physiologically. We presume that the interstitial accumulation of lymphocytes in lungs of patients with CF, which leads to a peripheral decrease of these cells, is increased. It is possible, that the pulmonary homing of CD4(+)CD45(+)R0-cells is a part of the pathophysiology of the lung disease in patients with CF. An immune response dominated by CD4(+)-cells may contribute to the persistence of the pulmonary inflammation. Possibly, immunophenotyping of peripheral blood LS will become a useful indirect tool for assessment of interstitial pulmonary inflammation in CF."]},{"key":"dc:source","label":"Dc Source","values":["Aachen : Publikationsserver der RWTH Aachen University 97 S. : Ill., graph. Darst. (2002). = Aachen, Techn. Hochsch., Diss., 2002"]},{"key":"dc:title","label":"Title","values":["Untersuchung zu Veränderungen der Lymphozytensubpopulationen im Krankheitsverlauf der Mukoviszidose"]}]}],"canonical_facts":{"dc:contributor":["Heimann, Gerhard"],"dc:coverage":["DE"],"dc:creator":["Opladen, Thomas"],"dc:date":["2002"],"dc:description":["Lung disease in cystic fibrosis (CF) is characterized by intrabronchial and interstitial inflammation. Compared with the intrabronchial compartment, knowledge on the lymphocyte system and on interstitial pneumonia in CF is limited. As changes of peripheral blood lymphocyte subsets (LS) may accompany severe systemic lymphocyte immune responses, we compared peripheral LS of 44 patients with CF, 23 non-CF Patients with recurrent pulmonary infections and 83 healthy controls. We performed lymphocyte phenotyping with CD3-, CD19-, CD16-, CD56-, CD4-, CD8-, CD11b-, CD57-, HLA-DR-, CD25-, CD45RA-, CD45RO- antigens. Additional immunohistochemistry was performed on lung tissue of four CF patients aged 0.5, 12, 17 and 20 years. We analyzed age depending and age independent differences between the three mentioned groups. Additionally we investigated the age depending changes within the patients with CF and the healthy controls. Comparison of patients and controls showed a marked lowering of CD4(+)CD45RO(+)-memory-cells, of T cells expressing IL-2R, of CD8(+) cells and of CD 16(+)56(+)-cells. Compared to healthy controls patients with recurrent broonchopulmonary infections showed similar changes. Immunohistochemistry showed activation of bronchus-associated lymphoid tissue with interstitial accumulation of CD4(+)CD45RO(+)-T-cells in the three older patients. This investigation is the first to demonstrate marked changes of peripheral lymphocyte subsets in patients with CF. As shown by the group of non-CF patients with pulmonary infections the registered changes of the lymphocyte subsets are not specific for CF. Furthermore the mentioned pulmonary homing of CD4(+)CD45RO(+)-cells seems to be physiologically. We presume that the interstitial accumulation of lymphocytes in lungs of patients with CF, which leads to a peripheral decrease of these cells, is increased. It is possible, that the pulmonary homing of CD4(+)CD45(+)R0-cells is a part of the pathophysiology of the lung disease in patients with CF. An immune response dominated by CD4(+)-cells may contribute to the persistence of the pulmonary inflammation. Possibly, immunophenotyping of peripheral blood LS will become a useful indirect tool for assessment of interstitial pulmonary inflammation in CF."],"dc:identifier":["https://publications.rwth-aachen.de/record/57215","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-119273%22"],"dc:language":["ger"],"dc:publisher":["Publikationsserver der RWTH Aachen University"],"dc:relation":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-4782"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:source":["Aachen : Publikationsserver der RWTH Aachen University 97 S. : Ill., graph. Darst. (2002). = Aachen, Techn. Hochsch., Diss., 2002"],"dc:subject":["info:eu-repo/classification/ddc/610","Medizin","Lymphozytensubpopulationen","Mukoviszidose"],"dc:title":["Untersuchung zu Veränderungen der Lymphozytensubpopulationen im Krankheitsverlauf der Mukoviszidose"],"dc:type":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]},"updated_at":"2026-07-30T19:42:09Z"}