Immunhistochemische Untersuchung von Integrinen auf Endothelzellen in Abhängigkeit von der extrazellulären Matrix und von verschiedenen stimulierenden Substanzen
type: CreativeWork
description: In this study we examined the expression of integrins on human venous endothelial cells (HUVEC) after adhesion to different matrix proteins by immunofluorescence microscopy. Additionally, we compared the influence of several stimulating factors (Phorbol-12-Myristate-13-Acetate/ PMA, Forskolin, Basic Fibroblast Growth Factor/ BFGF, Vascular Endothelial Growth Factor/ VEGF) on the expression of integrins. In the second part of the study we investigated the changes of cell morphology and integrin distribution after incubation with fibrin and after co-incubation with fibrin and the stimulating factors. Endothelial cells (EC) represent an important functional part of the vascular system. They actively participate in inflammatory reactions, hemostasis and in the formation of new blood vessels (angiogenesis). EC express integrin receptors on their cell membrane that promote their attachment to subendothelial matrix proteins. After contact with adhesive proteins these integrin molecules are clustered in focal adhesions (FA). The focal contacts depend on the composition of the extracellular matrix (ECM). Our data show that EC adhere to fibronectin via the alpha 5 beta 1 integrin and to vitronectin and fibrin via the alpha v beta 3 integrin. EC on vitronectin produce endogenous fibronectin which they secrete as an additional matrix protein and to which they adhere via alpha 5 beta 1 integrins. After activation of proteinkinase C (PKC) by PMA we see an increased alpha v beta 3-integrin-expression on fibronectin as well as on vitronectin. After stimulation with forskolin we find a temporary loss of the typical FA pattern on both matrix proteins. Stimulation with angiogenic growth factors (BFGF, VEGF) for 12 hours does not influence the expression of alpha 5 beta 1- and alpha v beta 3 integrins. After contact with a fibrin clot EC retract and the normal cobblestone monolayer architecture of cultured vascular endothelium is disrupted. This process is mediated by integrins. They i…
abstract: In this study we examined the expression of integrins on human venous endothelial cells (HUVEC) after adhesion to different matrix proteins by immunofluorescence microscopy. Additionally, we compared the influence of several stimulating factors (Phorbol-12-Myristate-13-Acetate/ PMA, Forskolin, Basic Fibroblast Growth Factor/ BFGF, Vascular Endothelial Growth Factor/ VEGF) on the expression of integrins. In the second part of the study we investigated the changes of cell morphology and integrin distribution after incubation with fibrin and after co-incubation with fibrin and the stimulating factors. Endothelial cells (EC) represent an important functional part of the vascular system. They actively participate in inflammatory reactions, hemostasis and in the formation of new blood vessels (angiogenesis). EC express integrin receptors on their cell membrane that promote their attachment to subendothelial matrix proteins. After contact with adhesive proteins these integrin molecules are clustered in focal adhesions (FA). The focal contacts depend on the composition of the extracellular matrix (ECM). Our data show that EC adhere to fibronectin via the alpha 5 beta 1 integrin and to vitronectin and fibrin via the alpha v beta 3 integrin. EC on vitronectin produce endogenous fibronectin which they secrete as an additional matrix protein and to which they adhere via alpha 5 beta 1 integrins. After activation of proteinkinase C (PKC) by PMA we see an increased alpha v beta 3-integrin-expression on fibronectin as well as on vitronectin. After stimulation with forskolin we find a temporary loss of the typical FA pattern on both matrix proteins. Stimulation with angiogenic growth factors (BFGF, VEGF) for 12 hours does not influence the expression of alpha 5 beta 1- and alpha v beta 3 integrins. After contact with a fibrin clot EC retract and the normal cobblestone monolayer architecture of cultured vascular endothelium is disrupted. This process is mediated by integrins. They i…
author: Heymanns, Ulrike
language: ger
keywords: info:eu-repo/classification/ddc/610, Medizin
date published: 2002
date modified: 2026-07-30T19:42:01Z
provider: RWTH Aachen University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Publikationsserver der RWTH Aachen University