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These technologies were successfully combined into strategies for the efficient isolation of three His6-tagged recombinant proteins (FSH-specific scFv4813, CEA N-A3 hybrid protein and CEA-specific scFvT84.66) from crude yeast fermentation broth or plant cell extract, with special emphasis on scalability and cost-effectiveness. Expanded bed IMAC was successfully introduced as a novel method for capture of recombinant proteins from plant cell extract. On-line monitoring of recombinant protein purification from P.pastoris fermentation broth by real-time BIA significantly reduced overall process times. The purified proteins were analysed with respect to purity, integrity and reactivity. The aims of this thesis were achieved by the expression and downstream processing of recombinant therapeutic proteins from P.pastoris fermentation broth and N.tabacum BY-2 suspension cells. 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