{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:52604"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:52604","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Topographische und funktionelle Charakterisierung des putativen Chaperons fls485","abstract":"Proteomics – the investigation of proteins, their composition and interaction in cells, has become an important scientific topic since the decoding of the human genom. This thesis also examines a protein, fls485, whose genetic code was discovered in a RNA-Genechiparray on human enterocytes of the small intestine. The sequence analysis of fls485 revealed two interesting motifs: -CXXCXGXG- , known as a specific motif for chaperons, and -CXXC-, which belongs to the members of the thiol-disulfide-oxidoreductase-family [Reinartz, Ehling et al., 2010]. Based on this observation, several issues are of particular importance: What are the tasks that are fulfilled by fls5485 inside a cell? Are there any tissue-specific preferences of this protein and, if so, can we conclude any more influences of fls485 on the tissue of interest or single specialized cells? Since the literature available about this topic is still incomplete, the characterization of fls485 was accomplished via two sides: The topographic and the functional analysis. The methods applied for the topographic analysis included immunohistochemistry, immunofluorescence on cell lines with known fls485 expression and electron microscopical immunogoldtechnique. Interestingly, this analysis revealed on the one hand an ubiquitous existence of fls485 in human tissues, on the other hand there is an increased expression in cells of ectodermal origin, for example epidermal cells or cells which belong to the diffuse neuroendocrine system. Considering these results, we hypothesized how the expression might change in pathologically altered neuroendocrine cells. Here we found a decreased expression of fls485 in neuroendocrine lesions dependent on their grade of differentiation: The less differentiated a tumor was, the less fls485 expression was detectable. This knowledge possibly supports the assumption other groups described, namely that fls485 acts as a putative tumorsuppressor gene in other tumor entities [Tschentscher et al., 2003]. Additionally, the data obtained by immunofluorescence and electron microscopy revealed a cytoplasmatic localization of fls485 without any nuclear expression. For the functional analysis an appropriate transfection model was established using two different cell lines (Capan-1 and 3T3). First results showed a decreased growth rate in cells with a fls485 overexpression, which supports the immunohistochemical data and indicatesa decelaration of the cell cycle's progress by fls485. To which extend fls485 as a putative chaperon affects the thiol-disulfide-oxidoreductase-system remains subject to further investigation and can be obtained by using the established model.","abstract_html":"Proteomics – the investigation of proteins, their composition and interaction in cells, has become an important scientific topic since the decoding of the human genom. This thesis also examines a protein, fls485, whose genetic code was discovered in a RNA-Genechiparray on human enterocytes of the small intestine. The sequence analysis of fls485 revealed two interesting motifs: -CXXCXGXG- , known as a specific motif for chaperons, and -CXXC-, which belongs to the members of the thiol-disulfide-oxidoreductase-family [Reinartz, Ehling et al., 2010]. Based on this observation, several issues are of particular importance: What are the tasks that are fulfilled by fls5485 inside a cell? Are there any tissue-specific preferences of this protein and, if so, can we conclude any more influences of fls485 on the tissue of interest or single specialized cells? Since the literature available about this topic is still incomplete, the characterization of fls485 was accomplished via two sides: The topographic and the functional analysis. The methods applied for the topographic analysis included immunohistochemistry, immunofluorescence on cell lines with known fls485 expression and electron microscopical immunogoldtechnique. Interestingly, this analysis revealed on the one hand an ubiquitous existence of fls485 in human tissues, on the other hand there is an increased expression in cells of ectodermal origin, for example epidermal cells or cells which belong to the diffuse neuroendocrine system. Considering these results, we hypothesized how the expression might change in pathologically altered neuroendocrine cells. Here we found a decreased expression of fls485 in neuroendocrine lesions dependent on their grade of differentiation: The less differentiated a tumor was, the less fls485 expression was detectable. This knowledge possibly supports the assumption other groups described, namely that fls485 acts as a putative tumorsuppressor gene in other tumor entities [Tschentscher et al., 2003]. Additionally, the data obtained by immunofluorescence and electron microscopy revealed a cytoplasmatic localization of fls485 without any nuclear expression. For the functional analysis an appropriate transfection model was established using two different cell lines (Capan-1 and 3T3). First results showed a decreased growth rate in cells with a fls485 overexpression, which supports the immunohistochemical data and indicatesa decelaration of the cell cycle's progress by fls485. To which extend fls485 as a putative chaperon affects the thiol-disulfide-oxidoreductase-system remains subject to further investigation and can be obtained by using the established model.","abstract_has_math":false,"creators":["Simon, Verena Anna Sophia"],"institution":"Publikationsserver der RWTH Aachen University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Gaßler, Nikolaus"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011","date_published":"2011","updated_at":"2026-07-30T19:41:00Z","subjects":["info:eu-repo/classification/ddc/610","Polypeptidketten bindende Proteine","Neuroendokriner Tumor","Medizin","fls485","Krypten-Zotten-Achse","chaperone","NET","CVA"],"languages":["ger"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-114816%22"],"render_values":[{"text":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-114816%22","href":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-114816%22","code":true}]}]},"links":{"outbound_url":"https://publications.rwth-aachen.de/record/52604","outbound_label":"Repository record","outbound_source":"dc:identifier"},"source_record":{"url":"https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A52604","prefix":"oai_dc"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Gaßler, Nikolaus"]},{"key":"dc:creator","label":"Author","values":["Simon, Verena Anna Sophia"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:coverage","label":"Dc Coverage","values":["DE"]},{"key":"dc:date","label":"Dc Date","values":["2011"]},{"key":"dc:publisher","label":"Institution","values":["Publikationsserver der RWTH Aachen University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-38912"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["info:eu-repo/classification/ddc/610","Polypeptidketten bindende Proteine","Neuroendokriner Tumor","Medizin","fls485","Krypten-Zotten-Achse","chaperone","NET","CVA"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ger"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/record/52604","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-114816%22"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Proteomics – the investigation of proteins, their composition and interaction in cells, has become an important scientific topic since the decoding of the human genom. This thesis also examines a protein, fls485, whose genetic code was discovered in a RNA-Genechiparray on human enterocytes of the small intestine. The sequence analysis of fls485 revealed two interesting motifs: -CXXCXGXG- , known as a specific motif for chaperons, and -CXXC-, which belongs to the members of the thiol-disulfide-oxidoreductase-family [Reinartz, Ehling et al., 2010]. Based on this observation, several issues are of particular importance: What are the tasks that are fulfilled by fls5485 inside a cell? Are there any tissue-specific preferences of this protein and, if so, can we conclude any more influences of fls485 on the tissue of interest or single specialized cells? Since the literature available about this topic is still incomplete, the characterization of fls485 was accomplished via two sides: The topographic and the functional analysis. The methods applied for the topographic analysis included immunohistochemistry, immunofluorescence on cell lines with known fls485 expression and electron microscopical immunogoldtechnique. Interestingly, this analysis revealed on the one hand an ubiquitous existence of fls485 in human tissues, on the other hand there is an increased expression in cells of ectodermal origin, for example epidermal cells or cells which belong to the diffuse neuroendocrine system. Considering these results, we hypothesized how the expression might change in pathologically altered neuroendocrine cells. Here we found a decreased expression of fls485 in neuroendocrine lesions dependent on their grade of differentiation: The less differentiated a tumor was, the less fls485 expression was detectable. This knowledge possibly supports the assumption other groups described, namely that fls485 acts as a putative tumorsuppressor gene in other tumor entities [Tschentscher et al., 2003]. Additionally, the data obtained by immunofluorescence and electron microscopy revealed a cytoplasmatic localization of fls485 without any nuclear expression. For the functional analysis an appropriate transfection model was established using two different cell lines (Capan-1 and 3T3). First results showed a decreased growth rate in cells with a fls485 overexpression, which supports the immunohistochemical data and indicatesa decelaration of the cell cycle's progress by fls485. To which extend fls485 as a putative chaperon affects the thiol-disulfide-oxidoreductase-system remains subject to further investigation and can be obtained by using the established model."]},{"key":"dc:source","label":"Dc Source","values":["Aachen : Publikationsserver der RWTH Aachen University III, 89 S. : Ill., graph. Darst. (2011). = Aachen, Techn. Hochsch., Diss., 2011"]},{"key":"dc:title","label":"Title","values":["Topographische und funktionelle Charakterisierung des putativen Chaperons fls485"]}]}],"canonical_facts":{"dc:contributor":["Gaßler, Nikolaus"],"dc:coverage":["DE"],"dc:creator":["Simon, Verena Anna Sophia"],"dc:date":["2011"],"dc:description":["Proteomics – the investigation of proteins, their composition and interaction in cells, has become an important scientific topic since the decoding of the human genom. This thesis also examines a protein, fls485, whose genetic code was discovered in a RNA-Genechiparray on human enterocytes of the small intestine. The sequence analysis of fls485 revealed two interesting motifs: -CXXCXGXG- , known as a specific motif for chaperons, and -CXXC-, which belongs to the members of the thiol-disulfide-oxidoreductase-family [Reinartz, Ehling et al., 2010]. Based on this observation, several issues are of particular importance: What are the tasks that are fulfilled by fls5485 inside a cell? Are there any tissue-specific preferences of this protein and, if so, can we conclude any more influences of fls485 on the tissue of interest or single specialized cells? Since the literature available about this topic is still incomplete, the characterization of fls485 was accomplished via two sides: The topographic and the functional analysis. The methods applied for the topographic analysis included immunohistochemistry, immunofluorescence on cell lines with known fls485 expression and electron microscopical immunogoldtechnique. Interestingly, this analysis revealed on the one hand an ubiquitous existence of fls485 in human tissues, on the other hand there is an increased expression in cells of ectodermal origin, for example epidermal cells or cells which belong to the diffuse neuroendocrine system. Considering these results, we hypothesized how the expression might change in pathologically altered neuroendocrine cells. Here we found a decreased expression of fls485 in neuroendocrine lesions dependent on their grade of differentiation: The less differentiated a tumor was, the less fls485 expression was detectable. This knowledge possibly supports the assumption other groups described, namely that fls485 acts as a putative tumorsuppressor gene in other tumor entities [Tschentscher et al., 2003]. Additionally, the data obtained by immunofluorescence and electron microscopy revealed a cytoplasmatic localization of fls485 without any nuclear expression. For the functional analysis an appropriate transfection model was established using two different cell lines (Capan-1 and 3T3). First results showed a decreased growth rate in cells with a fls485 overexpression, which supports the immunohistochemical data and indicatesa decelaration of the cell cycle's progress by fls485. 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