Invasionshemmung von Magentumorzellen nach Behandlung mit Phospholipiden in vitro

Tumors of the peritoneal cavity, such as castric cancer can cause a peritoneal carcinomatosis. Patients have a median survival of six month from time of diagnosis. The peritoneal carcinomatosis leaves no curative therapeutic option. Therefore the prevention of the PC should be the main goal. There are several diffenrent initiatives to prevent tumor cells from metastate to the peritoneum. The curativeness of the surgeon has to be the major part to keep large amounts of tumor cells from dropping into the peritoneal cavity during surgery. But evan in operations performed in so called “no touch technic” studies showed that there were living tumor cells in 90% of the probes. Several substances such as chemotherapeutics, immunologics and hyperthermia has been tested to reduce the tumor cell adheasion and migration. Many of the tested substances showed severe side effects and toxicity even in animal studies. Phospholipids showed a low toxicity and very good acceptance in clinical trials before. Goal of this work was to show the effects of phospholipids to the migration of gastic cancer cells in vitro. We tested the migration of the tumor cells at a boyden chamber assay. Therefore we pretreted the tumor cells in three different test arrangements with two different PL concentrations. The different test arrangement should imitate the peritoneal cavity with its extracellular matrix. After 24 hours we stoped the trial and counted the cells that migrated through a filter membrane with micropores. For all three test arrangement we received a statitical significant reduction of tumor cell migration. Furthermore we could investigate a trend of migration inhibition for higher doses but without statistical significance. In this study we were able to proof the reduction of migration by phospholipids in vitro. Further clinical studies shoud be performed to show the savety and evectiveness of phospholipids in vivo.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2010

Author dc:creator

• Heck, Andreas Alexander Konstantin

Contributors dc:contributor

• Jansen, Marc

• info:eu-repo/classification/ddc/610
• Phospholipide
• Magentumor
• Zellkultur
• In vitro
• In-vitro-Kultur
• Zellmigration
• Medizin
• Boydenkammer
• Migrationsreduktion
• reduction
• migration
• phospholipids
• tumor cell
• gastric cancer

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-114058%22

OAI record oai:identifier

https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A51806