{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:51715"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:51715","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Investigations on the role of mitogen-activated protein kinases 11 and 17 in defense response of Arabidopsis thaliana","abstract":"Priming of defense for a stronger and faster response to abiotic and biotic stress is an important part of acquired immunity in plants and animals. Recent findings revealed the involvement of the two mitogen-activated protein kinases MPK3 and MPK6 in the regulation of priming in Arabidopsis thaliana. Since the A. thaliana genome encodes 20 MPKs, I wondered, whether one or more of the other MPKs would be involved in priming. Bioinformatic analysis revealed a strong transcriptional upregulation of MPK11 upon treatment with the priming-inducing chemicals BTH, BABA, and salicylic acid, and upon several biotic and abiotic stress stimuli. In addition, I performed a phenotypic screen of mpk mutants that revealed strong morphological defects in mpk17 T-DNA knockout plants. To elucidate the potential role of MPK11 and MPK17 in plant defense, I treated mpk11 and mpk17 mutants with various plant pathogens and analyzed basal plant defense responses. Furthermore, I performed drought stress experiments to investigate one abiotic stress response in mpk11 and mpk17 mutants. I found that both mutants are not affected in their response against Pseudomonas syringae and the necrotrophic fungus Botrytis cinerea, whereas I could reveal a slight reduction in basal defense against the biotrophic oomycete Hyaloperonospora arabidopsidis. In addition, I showed that both mutants are not affected in the production of reactive oxygen species or in deposition of callose, two typical basal defense responses. Furthermore, by analysis of protein phosphorylation, I excluded a compensational effect of MPK3, MPK4, and MPK6, the major players in plant defense signaling, for the loss of mpk11 and mpk17. In all experiments, I did not see significant alterations in primed defense responses, suggesting that both kinases do, if at all, only play a minor role in plant defense. According to bioinformatic analysis, I propose a role for MPK17 in the regulation of developmental processes. Furthermore, I discuss a possible role of MPK11 as a backup system for other kinases.","abstract_html":"Priming of defense for a stronger and faster response to abiotic and biotic stress is an important part of acquired immunity in plants and animals. Recent findings revealed the involvement of the two mitogen-activated protein kinases MPK3 and MPK6 in the regulation of priming in Arabidopsis thaliana. Since the A. thaliana genome encodes 20 MPKs, I wondered, whether one or more of the other MPKs would be involved in priming. Bioinformatic analysis revealed a strong transcriptional upregulation of MPK11 upon treatment with the priming-inducing chemicals BTH, BABA, and salicylic acid, and upon several biotic and abiotic stress stimuli. In addition, I performed a phenotypic screen of mpk mutants that revealed strong morphological defects in mpk17 T-DNA knockout plants. To elucidate the potential role of MPK11 and MPK17 in plant defense, I treated mpk11 and mpk17 mutants with various plant pathogens and analyzed basal plant defense responses. Furthermore, I performed drought stress experiments to investigate one abiotic stress response in mpk11 and mpk17 mutants. I found that both mutants are not affected in their response against Pseudomonas syringae and the necrotrophic fungus Botrytis cinerea, whereas I could reveal a slight reduction in basal defense against the biotrophic oomycete Hyaloperonospora arabidopsidis. In addition, I showed that both mutants are not affected in the production of reactive oxygen species or in deposition of callose, two typical basal defense responses. Furthermore, by analysis of protein phosphorylation, I excluded a compensational effect of MPK3, MPK4, and MPK6, the major players in plant defense signaling, for the loss of mpk11 and mpk17. In all experiments, I did not see significant alterations in primed defense responses, suggesting that both kinases do, if at all, only play a minor role in plant defense. According to bioinformatic analysis, I propose a role for MPK17 in the regulation of developmental processes. Furthermore, I discuss a possible role of MPK11 as a backup system for other kinases.","abstract_has_math":false,"creators":["Neumann, Martin"],"institution":"Publikationsserver der RWTH Aachen University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Conrath, Uwe"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011","date_published":"2011","updated_at":"2026-07-30T19:40:42Z","subjects":["info:eu-repo/classification/ddc/570","MAP-Kinase","Ackerschmalwand","Abwehrreaktion","Biowissenschaften, Biologie","Proteinkinase","Arabidopsis","plant defense"],"languages":["eng"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113977%22"],"render_values":[{"text":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113977%22","href":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113977%22","code":true}]}]},"links":{"outbound_url":"https://publications.rwth-aachen.de/record/51715","outbound_label":"Repository record","outbound_source":"dc:identifier"},"source_record":{"url":"https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A51715","prefix":"oai_dc"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Conrath, Uwe"]},{"key":"dc:creator","label":"Author","values":["Neumann, Martin"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:coverage","label":"Dc Coverage","values":["DE"]},{"key":"dc:date","label":"Dc Date","values":["2011"]},{"key":"dc:publisher","label":"Institution","values":["Publikationsserver der RWTH Aachen University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-38962"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["info:eu-repo/classification/ddc/570","MAP-Kinase","Ackerschmalwand","Abwehrreaktion","Biowissenschaften, Biologie","Proteinkinase","Arabidopsis","plant defense"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/record/51715","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113977%22"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Priming of defense for a stronger and faster response to abiotic and biotic stress is an important part of acquired immunity in plants and animals. Recent findings revealed the involvement of the two mitogen-activated protein kinases MPK3 and MPK6 in the regulation of priming in Arabidopsis thaliana. Since the A. thaliana genome encodes 20 MPKs, I wondered, whether one or more of the other MPKs would be involved in priming. Bioinformatic analysis revealed a strong transcriptional upregulation of MPK11 upon treatment with the priming-inducing chemicals BTH, BABA, and salicylic acid, and upon several biotic and abiotic stress stimuli. In addition, I performed a phenotypic screen of mpk mutants that revealed strong morphological defects in mpk17 T-DNA knockout plants. To elucidate the potential role of MPK11 and MPK17 in plant defense, I treated mpk11 and mpk17 mutants with various plant pathogens and analyzed basal plant defense responses. Furthermore, I performed drought stress experiments to investigate one abiotic stress response in mpk11 and mpk17 mutants. I found that both mutants are not affected in their response against Pseudomonas syringae and the necrotrophic fungus Botrytis cinerea, whereas I could reveal a slight reduction in basal defense against the biotrophic oomycete Hyaloperonospora arabidopsidis. In addition, I showed that both mutants are not affected in the production of reactive oxygen species or in deposition of callose, two typical basal defense responses. Furthermore, by analysis of protein phosphorylation, I excluded a compensational effect of MPK3, MPK4, and MPK6, the major players in plant defense signaling, for the loss of mpk11 and mpk17. In all experiments, I did not see significant alterations in primed defense responses, suggesting that both kinases do, if at all, only play a minor role in plant defense. According to bioinformatic analysis, I propose a role for MPK17 in the regulation of developmental processes. Furthermore, I discuss a possible role of MPK11 as a backup system for other kinases."]},{"key":"dc:source","label":"Dc Source","values":["Aachen : Publikationsserver der RWTH Aachen University V, 112 Bl. : Ill., graph. Darst. (2011). = Aachen, Techn. Hochsch., Diss., 2011"]},{"key":"dc:title","label":"Title","values":["Investigations on the role of mitogen-activated protein kinases 11 and 17 in defense response of Arabidopsis thaliana"]}]}],"canonical_facts":{"dc:contributor":["Conrath, Uwe"],"dc:coverage":["DE"],"dc:creator":["Neumann, Martin"],"dc:date":["2011"],"dc:description":["Priming of defense for a stronger and faster response to abiotic and biotic stress is an important part of acquired immunity in plants and animals. Recent findings revealed the involvement of the two mitogen-activated protein kinases MPK3 and MPK6 in the regulation of priming in Arabidopsis thaliana. Since the A. thaliana genome encodes 20 MPKs, I wondered, whether one or more of the other MPKs would be involved in priming. Bioinformatic analysis revealed a strong transcriptional upregulation of MPK11 upon treatment with the priming-inducing chemicals BTH, BABA, and salicylic acid, and upon several biotic and abiotic stress stimuli. In addition, I performed a phenotypic screen of mpk mutants that revealed strong morphological defects in mpk17 T-DNA knockout plants. To elucidate the potential role of MPK11 and MPK17 in plant defense, I treated mpk11 and mpk17 mutants with various plant pathogens and analyzed basal plant defense responses. Furthermore, I performed drought stress experiments to investigate one abiotic stress response in mpk11 and mpk17 mutants. I found that both mutants are not affected in their response against Pseudomonas syringae and the necrotrophic fungus Botrytis cinerea, whereas I could reveal a slight reduction in basal defense against the biotrophic oomycete Hyaloperonospora arabidopsidis. In addition, I showed that both mutants are not affected in the production of reactive oxygen species or in deposition of callose, two typical basal defense responses. Furthermore, by analysis of protein phosphorylation, I excluded a compensational effect of MPK3, MPK4, and MPK6, the major players in plant defense signaling, for the loss of mpk11 and mpk17. In all experiments, I did not see significant alterations in primed defense responses, suggesting that both kinases do, if at all, only play a minor role in plant defense. According to bioinformatic analysis, I propose a role for MPK17 in the regulation of developmental processes. Furthermore, I discuss a possible role of MPK11 as a backup system for other kinases."],"dc:identifier":["https://publications.rwth-aachen.de/record/51715","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113977%22"],"dc:language":["eng"],"dc:publisher":["Publikationsserver der RWTH Aachen University"],"dc:relation":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-38962"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:source":["Aachen : Publikationsserver der RWTH Aachen University V, 112 Bl. : Ill., graph. Darst. (2011). = Aachen, Techn. Hochsch., Diss., 2011"],"dc:subject":["info:eu-repo/classification/ddc/570","MAP-Kinase","Ackerschmalwand","Abwehrreaktion","Biowissenschaften, Biologie","Proteinkinase","Arabidopsis","plant defense"],"dc:title":["Investigations on the role of mitogen-activated protein kinases 11 and 17 in defense response of Arabidopsis thaliana"],"dc:type":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]},"updated_at":"2026-07-30T19:40:42Z"}