Zusammenhang zwischen methanogenen Archaea und sulfatreduzierenden Mikroorganismen bei Parodontitis

One of the three domains of life consists of the members of the Archaea group. Despite their numbers and their overall presence on the planet and their close association with humans, animals and plants, no pathogenic archaea have as yet been described. Some archaea are found close to bacteria that exist in anaerobic conditions e.g. in the human bowel or in periodontal pockets. But until today, their physiological role was not cleared. A periodontal disease is an anaerobic infection caused by polymicrobes, characterised by the definite establishment of a highly complex microbial subgingival ecosystem (plaque). This biofilm, together with the host’s specific (risk factors that are acquired or based on lifestyle) immunological and inflammatory reaction can lead to the deterioration of the periodontium and lately to the loss of the tooth itself. Initial examinations proved that methan-producing (methanogenic) archaea and sulfate-reducing micro-organisms are present in periodontal pockets. Up to now these both groups of organisms have only been detected in natural habitats. This was a reason to carry out a comprehensive study to determine which kind of these micro-organisms and which quantity can be detected in the destructive processes of the periodontium, whether they interact with other micro-organisms and if there is an indication of the aetiological role these organisms play in periodontal diseases. Samples were taken from 102 patients in different stages of periodontal disease and analyzed with the help of real time quantitative PCR (polymerase chain reaction). They were compared to samples taken from a control group of 65 healthy subjects. Functional genes specifically for methanogenic archaea and for dissimilatory sulfate-reducers allowing the detection of physiological distinct microorganisms have been used. Detectable levels of methanogens and sulfate-reducers were only found at diseased sites, with proportions of them being significantly elevated in the more severe cases. Although this finding demonstrates association with, rather than causation of, disease, the increased importance of both groups for the progression of periodontitis is evident and principally designates the functional as potential biomarkers for progressive periodontal disease with high positive predictive values. To investigate the cause of periodontitis furthermore, it should be necessary to regard the total structure of the micro-organisms more intensively, because none of the pathogenes seem to be responsible all by itself. Completing studies should give more importance to the realization of those basic principles, which accelerate the infectuous process in spite of the specific structure of host or site. This might even increase the understanding of periodontitis significantly.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2009

Author dc:creator

• Holtgräwe, Simone Mareen

Contributors dc:contributor

• Conrads, Georg

• info:eu-repo/classification/ddc/610
• Methanbakterien
• Sulfatreduzierer
• Biofilm
• Real time quantitative PCR
• Medizin
• sulfatreduzierende Bakterien
• methanogene Archaea
• subgingivaler Biofilm
• quantitative Real-time Polymerase-Kettenreaktion
• sulfate-reducing bacteria
• subgingival plaque
• real-time quantitative polymerase chain reaction

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113763%22

OAI record oai:identifier

https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A51474