Molekulare Markierungsstrategien für die optische und magnetresonanztomographische zelluläre Visualisierung

Cellular labelling for molecular and multimodal visualisation was designed and validated. Of interest was the connection of optical detection and magnetic resonance imaging to combine quantitative with three dimensional information. Detection and quantification of cell amounts below 1000 cells were focused. Enhanced fluorescence proteins were overexpressed for optical imaging and for bioluminescence imaging firefly luciferase was used. A fluorescence protein with a longer emission wavelength was used to enable further efficient in vivo studies. Cells were labeled with superparamagnetic iron oxide particles for the MRI detection and uptake and distribution of the particles was investigated. For enhanced labeling of cells a liposomal based strategy was implemented and modified. Overexpression of human heavy chain ferritin in combination with firefly luciferase in glioma cells led to a cell specific internal MR contrast in combination with bioluminescence detection. For efficient transduction a lentiviral system was used. Exchange of promoters is promising for cell or tissue specific expression. Another strategy was the overexpression of artifical surface receptors which were truncated at cytoplasmic site to avoid further signal protein interaction. Iron oxide particles, conjugated to antibodies enabled MRI localisation after antibody receptor binding. This extracellular labeling was determined for tumour cells and neural cells. Neural cells were differentiated from embryonic stem cells with modified and optimized protocols.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2009

Author dc:creator

• Kruttwig, Klaus

Contributors dc:contributor

• Zenke, Martin

• info:eu-repo/classification/ddc/570
• Molekulare Bildgebung
• NMR-Bildgebung
• Gliom
• Kontrastmittel
• Nervenzelle
• Embryonale Stammzelle
• Antikörper
• Biowissenschaften, Biologie
• molecular imaging
• NMR-imaging
• glioma
• contrast agent
• embryonic stem cells

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113725%22

OAI record oai:identifier

https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A51434