{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:51410"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:51410","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Dendritische Zellen bei der Intra- und Extrauteringravidität : mögliche Bedeutung für die feto-maternale Toleranz","abstract":"Dendritic cells (DC) are potent antigen-presenting cells. They are able to induce immunity on the one hand, on the other hand DC play a central role in the induction of peripheral tolerance. The presence of different maturation states of DC has been described at the feto-maternal implantation site of intrauterine pregnancies (IUP). It is assumed that the interaction of immature DC with natural killer (NK)-cells plays an important role for the induction and maintenance of feto-maternal tolerance. Mature DC are characterized to exhibit protective features but they might also be responsible for the development of intrauterine abortions by inducing a T-cell mediated immune response. Tubal pregnancies (TP) provide an important comparison: while the presence of mature and immature DC in IUP has been stated by several studies, there is little known about the presence of these cells in the extrauterine implantation site of TP. The TP is characterized by a different invasive behaviour of trophoblast cells and a different pattern of immune cells (e.g. absence of NK-cells). In this study for the first time the pattern of mature and immature DC at the implantation site of TP was analysed and compared to the pattern in the IUP. A differentiation was made between viable tubal pregnancies (VTP) and tubal abortions (TA). We examined seven specimens of decidual tissue obtained from women with intact IUP undergoing abortion in the 5.-9. week of gestation, ten specimens of VTP (identified by colour Doppler sonography) and five specimens of TA. VTP and TA were of the same gestational age as the IUP. Immunohistochemical staining protocols were established on paraffin sections. The following surface markers were examined on serial sections: 1. Cytokeratin 7 (identification of implantation site), 2. CD83 (mature DC), 3. DEC205 (immature, activated DC), 4. DC-SIGN (immature, macrophage-like transient state of DC), 5. CD14 (identifies monocytes/macrophages). For CD14 single labelling was performed as well as for some specimens double labelling with DC-SIGN. IUP and VTP show nearly the same pattern of mature and immature DC. Regarding the different maturation states examined DC-SIGN+ DC are found in highest numbers. Thus, DC-SIGN+ DC may be of importance for the establishment of feto-maternal tolerance in both entities. A high percentage of DC-SIGN+ cells are stained for the monocyte/macrophage marker CD14 as well. While immature, activated DEC205+ DC show intermediate cell numbers, very few mature CD83+ are found in the tissue. The fact that the different maturation states of DC are found equally in intrauterine and extrauterine site of trophoblast implantation suggests that DC play an important role for the immunological processes in TP as they do in IUP. As tissue of TP lacks NK-cells, DC-SIGN+ DC may interact with other immunological cells to establish feto-maternal tolerance than in IUP. The same pattern of DC is observed in TA and VTP. Thus it is unlikely that DC specifically influence the development of a VTP into a TA. As IUP and TP exhibit the same pattern of DC these cells are probably not responsible for the increased invasiveness of trophoblast cells in VTP. The specific role of DC and their interaction with other immunological cells in TP has to be analysed by further studies. This may clarify to what extent the DC plays a rather protective role in TP as it is assumed for IUP. Furthermore this may reflect how DC are part of the pathophysiological processes of VTP and TA.","abstract_html":"Dendritic cells (DC) are potent antigen-presenting cells. They are able to induce immunity on the one hand, on the other hand DC play a central role in the induction of peripheral tolerance. The presence of different maturation states of DC has been described at the feto-maternal implantation site of intrauterine pregnancies (IUP). It is assumed that the interaction of immature DC with natural killer (NK)-cells plays an important role for the induction and maintenance of feto-maternal tolerance. Mature DC are characterized to exhibit protective features but they might also be responsible for the development of intrauterine abortions by inducing a T-cell mediated immune response. Tubal pregnancies (TP) provide an important comparison: while the presence of mature and immature DC in IUP has been stated by several studies, there is little known about the presence of these cells in the extrauterine implantation site of TP. The TP is characterized by a different invasive behaviour of trophoblast cells and a different pattern of immune cells (e.g. absence of NK-cells). In this study for the first time the pattern of mature and immature DC at the implantation site of TP was analysed and compared to the pattern in the IUP. A differentiation was made between viable tubal pregnancies (VTP) and tubal abortions (TA). We examined seven specimens of decidual tissue obtained from women with intact IUP undergoing abortion in the 5.-9. week of gestation, ten specimens of VTP (identified by colour Doppler sonography) and five specimens of TA. VTP and TA were of the same gestational age as the IUP. Immunohistochemical staining protocols were established on paraffin sections. The following surface markers were examined on serial sections: 1. Cytokeratin 7 (identification of implantation site), 2. CD83 (mature DC), 3. DEC205 (immature, activated DC), 4. DC-SIGN (immature, macrophage-like transient state of DC), 5. CD14 (identifies monocytes/macrophages). For CD14 single labelling was performed as well as for some specimens double labelling with DC-SIGN. IUP and VTP show nearly the same pattern of mature and immature DC. Regarding the different maturation states examined DC-SIGN+ DC are found in highest numbers. Thus, DC-SIGN+ DC may be of importance for the establishment of feto-maternal tolerance in both entities. A high percentage of DC-SIGN+ cells are stained for the monocyte/macrophage marker CD14 as well. While immature, activated DEC205+ DC show intermediate cell numbers, very few mature CD83+ are found in the tissue. The fact that the different maturation states of DC are found equally in intrauterine and extrauterine site of trophoblast implantation suggests that DC play an important role for the immunological processes in TP as they do in IUP. As tissue of TP lacks NK-cells, DC-SIGN+ DC may interact with other immunological cells to establish feto-maternal tolerance than in IUP. The same pattern of DC is observed in TA and VTP. Thus it is unlikely that DC specifically influence the development of a VTP into a TA. As IUP and TP exhibit the same pattern of DC these cells are probably not responsible for the increased invasiveness of trophoblast cells in VTP. The specific role of DC and their interaction with other immunological cells in TP has to be analysed by further studies. This may clarify to what extent the DC plays a rather protective role in TP as it is assumed for IUP. Furthermore this may reflect how DC are part of the pathophysiological processes of VTP and TA.","abstract_has_math":false,"creators":["Schmitz, Svenja"],"institution":"Publikationsserver der RWTH Aachen University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Kemp, Birgit"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2009,"date_issued":"2009","date_published":"2009","updated_at":"2026-07-30T19:40:33Z","subjects":["info:eu-repo/classification/ddc/610","Extrauterinschwangerschaft","Dendritische Zelle","Immuncytochemie","Antigen CD83","Antigen CD209","Medizin","Antigen DEC205","Immunhistochemie","feto-maternale Toleranz","Ectopic pregnancy","dendritic cell","CD83","DEC205","DC-SIGN"],"languages":["ger"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113702%22"],"render_values":[{"text":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113702%22","href":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113702%22","code":true}]}]},"links":{"outbound_url":"https://publications.rwth-aachen.de/record/51410","outbound_label":"Repository record","outbound_source":"dc:identifier"},"source_record":{"url":"https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A51410","prefix":"oai_dc"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kemp, Birgit"]},{"key":"dc:creator","label":"Author","values":["Schmitz, Svenja"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:coverage","label":"Dc Coverage","values":["DE"]},{"key":"dc:date","label":"Dc Date","values":["2009"]},{"key":"dc:publisher","label":"Institution","values":["Publikationsserver der RWTH Aachen University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-29277"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["info:eu-repo/classification/ddc/610","Extrauterinschwangerschaft","Dendritische Zelle","Immuncytochemie","Antigen CD83","Antigen CD209","Medizin","Antigen DEC205","Immunhistochemie","feto-maternale Toleranz","Ectopic pregnancy","dendritic cell","CD83","DEC205","DC-SIGN"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ger"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/record/51410","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113702%22"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Dendritic cells (DC) are potent antigen-presenting cells. They are able to induce immunity on the one hand, on the other hand DC play a central role in the induction of peripheral tolerance. The presence of different maturation states of DC has been described at the feto-maternal implantation site of intrauterine pregnancies (IUP). It is assumed that the interaction of immature DC with natural killer (NK)-cells plays an important role for the induction and maintenance of feto-maternal tolerance. Mature DC are characterized to exhibit protective features but they might also be responsible for the development of intrauterine abortions by inducing a T-cell mediated immune response. Tubal pregnancies (TP) provide an important comparison: while the presence of mature and immature DC in IUP has been stated by several studies, there is little known about the presence of these cells in the extrauterine implantation site of TP. The TP is characterized by a different invasive behaviour of trophoblast cells and a different pattern of immune cells (e.g. absence of NK-cells). In this study for the first time the pattern of mature and immature DC at the implantation site of TP was analysed and compared to the pattern in the IUP. A differentiation was made between viable tubal pregnancies (VTP) and tubal abortions (TA). We examined seven specimens of decidual tissue obtained from women with intact IUP undergoing abortion in the 5.-9. week of gestation, ten specimens of VTP (identified by colour Doppler sonography) and five specimens of TA. VTP and TA were of the same gestational age as the IUP. Immunohistochemical staining protocols were established on paraffin sections. The following surface markers were examined on serial sections: 1. Cytokeratin 7 (identification of implantation site), 2. CD83 (mature DC), 3. DEC205 (immature, activated DC), 4. DC-SIGN (immature, macrophage-like transient state of DC), 5. CD14 (identifies monocytes/macrophages). For CD14 single labelling was performed as well as for some specimens double labelling with DC-SIGN. IUP and VTP show nearly the same pattern of mature and immature DC. Regarding the different maturation states examined DC-SIGN+ DC are found in highest numbers. Thus, DC-SIGN+ DC may be of importance for the establishment of feto-maternal tolerance in both entities. A high percentage of DC-SIGN+ cells are stained for the monocyte/macrophage marker CD14 as well. While immature, activated DEC205+ DC show intermediate cell numbers, very few mature CD83+ are found in the tissue. The fact that the different maturation states of DC are found equally in intrauterine and extrauterine site of trophoblast implantation suggests that DC play an important role for the immunological processes in TP as they do in IUP. As tissue of TP lacks NK-cells, DC-SIGN+ DC may interact with other immunological cells to establish feto-maternal tolerance than in IUP. The same pattern of DC is observed in TA and VTP. Thus it is unlikely that DC specifically influence the development of a VTP into a TA. As IUP and TP exhibit the same pattern of DC these cells are probably not responsible for the increased invasiveness of trophoblast cells in VTP. The specific role of DC and their interaction with other immunological cells in TP has to be analysed by further studies. This may clarify to what extent the DC plays a rather protective role in TP as it is assumed for IUP. Furthermore this may reflect how DC are part of the pathophysiological processes of VTP and TA."]},{"key":"dc:source","label":"Dc Source","values":["Aachen : Publikationsserver der RWTH Aachen University 77 S. : Ill., graph. Darst. (2009). = Aachen, Techn. Hochsch., Diss., 2009"]},{"key":"dc:title","label":"Title","values":["Dendritische Zellen bei der Intra- und Extrauteringravidität : mögliche Bedeutung für die feto-maternale Toleranz"]}]}],"canonical_facts":{"dc:contributor":["Kemp, Birgit"],"dc:coverage":["DE"],"dc:creator":["Schmitz, Svenja"],"dc:date":["2009"],"dc:description":["Dendritic cells (DC) are potent antigen-presenting cells. They are able to induce immunity on the one hand, on the other hand DC play a central role in the induction of peripheral tolerance. The presence of different maturation states of DC has been described at the feto-maternal implantation site of intrauterine pregnancies (IUP). It is assumed that the interaction of immature DC with natural killer (NK)-cells plays an important role for the induction and maintenance of feto-maternal tolerance. Mature DC are characterized to exhibit protective features but they might also be responsible for the development of intrauterine abortions by inducing a T-cell mediated immune response. Tubal pregnancies (TP) provide an important comparison: while the presence of mature and immature DC in IUP has been stated by several studies, there is little known about the presence of these cells in the extrauterine implantation site of TP. The TP is characterized by a different invasive behaviour of trophoblast cells and a different pattern of immune cells (e.g. absence of NK-cells). In this study for the first time the pattern of mature and immature DC at the implantation site of TP was analysed and compared to the pattern in the IUP. A differentiation was made between viable tubal pregnancies (VTP) and tubal abortions (TA). We examined seven specimens of decidual tissue obtained from women with intact IUP undergoing abortion in the 5.-9. week of gestation, ten specimens of VTP (identified by colour Doppler sonography) and five specimens of TA. VTP and TA were of the same gestational age as the IUP. Immunohistochemical staining protocols were established on paraffin sections. The following surface markers were examined on serial sections: 1. Cytokeratin 7 (identification of implantation site), 2. CD83 (mature DC), 3. DEC205 (immature, activated DC), 4. DC-SIGN (immature, macrophage-like transient state of DC), 5. CD14 (identifies monocytes/macrophages). For CD14 single labelling was performed as well as for some specimens double labelling with DC-SIGN. IUP and VTP show nearly the same pattern of mature and immature DC. Regarding the different maturation states examined DC-SIGN+ DC are found in highest numbers. Thus, DC-SIGN+ DC may be of importance for the establishment of feto-maternal tolerance in both entities. A high percentage of DC-SIGN+ cells are stained for the monocyte/macrophage marker CD14 as well. While immature, activated DEC205+ DC show intermediate cell numbers, very few mature CD83+ are found in the tissue. The fact that the different maturation states of DC are found equally in intrauterine and extrauterine site of trophoblast implantation suggests that DC play an important role for the immunological processes in TP as they do in IUP. As tissue of TP lacks NK-cells, DC-SIGN+ DC may interact with other immunological cells to establish feto-maternal tolerance than in IUP. The same pattern of DC is observed in TA and VTP. Thus it is unlikely that DC specifically influence the development of a VTP into a TA. As IUP and TP exhibit the same pattern of DC these cells are probably not responsible for the increased invasiveness of trophoblast cells in VTP. The specific role of DC and their interaction with other immunological cells in TP has to be analysed by further studies. This may clarify to what extent the DC plays a rather protective role in TP as it is assumed for IUP. Furthermore this may reflect how DC are part of the pathophysiological processes of VTP and TA."],"dc:identifier":["https://publications.rwth-aachen.de/record/51410","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113702%22"],"dc:language":["ger"],"dc:publisher":["Publikationsserver der RWTH Aachen University"],"dc:relation":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-29277"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:source":["Aachen : Publikationsserver der RWTH Aachen University 77 S. : Ill., graph. Darst. (2009). = Aachen, Techn. Hochsch., Diss., 2009"],"dc:subject":["info:eu-repo/classification/ddc/610","Extrauterinschwangerschaft","Dendritische Zelle","Immuncytochemie","Antigen CD83","Antigen CD209","Medizin","Antigen DEC205","Immunhistochemie","feto-maternale Toleranz","Ectopic pregnancy","dendritic cell","CD83","DEC205","DC-SIGN"],"dc:title":["Dendritische Zellen bei der Intra- und Extrauteringravidität : mögliche Bedeutung für die feto-maternale Toleranz"],"dc:type":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]},"updated_at":"2026-07-30T19:40:33Z"}