{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:51355"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:51355","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Autoradiographische Untersuchungen zum Einfluss einer achtwöchigen 50-Hz-Magnetfeld-Exposition auf DNA-Reparatur und mitochondriale DNA-Synthese verschiedener Zellarten im Gehirn adulter Mäuse","abstract":"The aim of this study was to analyse the effects of a continuous exposure of 9 months old mice to a 50 Hz magnetic field (MF) with flux densities of 1 mT, 0,1 mT, or 0 mT (controls) on specific brain cells (epithelial cells of the choroids plexus of the II. and IV. Ventricle, granule cells of the hippocampal dentate gyrus and cortical endothelial cells. This was carried out by analysing autoradiographs after injection of ³H-thymidine (TdR) concerning nuclear (n) unscheduled DNA synthesis (UDS) and mitochondrial (mt) DNA synthesis in order to obtain information on MF induced nDNA damage and its repair as well as the cell metabolism. 80 male mice (NMRI strain) were randomly divided into four groups, In group I and II, the MF exposure was 1 mT (I) or 0.1 mT (II). Group III was the control group, i.e. sham-exposed with 0 mT. After the end of the MF-exposure or sham-exposure, the mice were injected with ³H-TdR, either after 5 minutes (Group A), or 24 hours (Group B) or 7 days (Group C). After paraffin embedding 3 µm thick paramedian, sagittal sections were cut, and subsequently autoradiographs prepared (exposure time: 250 d at 4°C). 100 consecutive cells per cell type and animal were analysed concerning nuclear and cytoplasmic grain numbers as well as the corresponding profile areas. For background correction cells of six mice without MF-exposure and without ³H-TdR injection were examined additionally. The statistical analyses included univariance analyses and subsequent post-hoc t-tests with so-called Bonferroni-corrections. Furthermore, the new data and those of a preceding pilot study (Schmitz et al., 2004) were combined in order to carry out regression analyses. Regarding the effects of the different MF doses on UDS, only the epithelial cells of the choroids plexus (of both ventricles) showed significant results. Surprisingly, UDS was significantly (p<0.05) smaller after exposure to 1 mT than 0 mT. This was probably caused by effects unrelated to the MF-exposure. In contrast, the results of linear and quadratic regression analyses led suppose that only MF-exposure with more than 1 mT leads to DNA damage in the epithelial cells of the choroids plexus. However, no harmful effects of MF-exposure could be detected on the hippocampal granule cells. The cytoplasmic labelling of the epithelial cells of the choroids plexus also showed a significant decline between 0 mT and 1 mT MF-exposure. Since the data obtained showed a good correlation with the quadratic regression model, it can be assumed that – as in the case of UDS – MF-exposure with a dose higher than 1 mT leads to an increased cellular metabolism.","abstract_html":"The aim of this study was to analyse the effects of a continuous exposure of 9 months old mice to a 50 Hz magnetic field (MF) with flux densities of 1 mT, 0,1 mT, or 0 mT (controls) on specific brain cells (epithelial cells of the choroids plexus of the II. and IV. Ventricle, granule cells of the hippocampal dentate gyrus and cortical endothelial cells. This was carried out by analysing autoradiographs after injection of ³H-thymidine (TdR) concerning nuclear (n) unscheduled DNA synthesis (UDS) and mitochondrial (mt) DNA synthesis in order to obtain information on MF induced nDNA damage and its repair as well as the cell metabolism. 80 male mice (NMRI strain) were randomly divided into four groups, In group I and II, the MF exposure was 1 mT (I) or 0.1 mT (II). Group III was the control group, i.e. sham-exposed with 0 mT. After the end of the MF-exposure or sham-exposure, the mice were injected with ³H-TdR, either after 5 minutes (Group A), or 24 hours (Group B) or 7 days (Group C). After paraffin embedding 3 µm thick paramedian, sagittal sections were cut, and subsequently autoradiographs prepared (exposure time: 250 d at 4°C). 100 consecutive cells per cell type and animal were analysed concerning nuclear and cytoplasmic grain numbers as well as the corresponding profile areas. For background correction cells of six mice without MF-exposure and without ³H-TdR injection were examined additionally. The statistical analyses included univariance analyses and subsequent post-hoc t-tests with so-called Bonferroni-corrections. Furthermore, the new data and those of a preceding pilot study (Schmitz et al., 2004) were combined in order to carry out regression analyses. Regarding the effects of the different MF doses on UDS, only the epithelial cells of the choroids plexus (of both ventricles) showed significant results. Surprisingly, UDS was significantly (p&lt;0.05) smaller after exposure to 1 mT than 0 mT. This was probably caused by effects unrelated to the MF-exposure. In contrast, the results of linear and quadratic regression analyses led suppose that only MF-exposure with more than 1 mT leads to DNA damage in the epithelial cells of the choroids plexus. However, no harmful effects of MF-exposure could be detected on the hippocampal granule cells. The cytoplasmic labelling of the epithelial cells of the choroids plexus also showed a significant decline between 0 mT and 1 mT MF-exposure. Since the data obtained showed a good correlation with the quadratic regression model, it can be assumed that – as in the case of UDS – MF-exposure with a dose higher than 1 mT leads to an increased cellular metabolism.","abstract_has_math":false,"creators":["Füller, Katja"],"institution":"Publikationsserver der RWTH Aachen University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Korr, Hubert"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2009,"date_issued":"2009","date_published":"2009","updated_at":"2026-07-30T19:40:33Z","subjects":["info:eu-repo/classification/ddc/610","Magnetfeldeffekt","DNS-Reparatur","DNS-Strangbruch","Autoradiographie","Mitochondriale DNS","Plexus chorioideus","Primärstoffwechsel","Magnetfeld","Tritium","Thymidin","Medizin","UDS","mitochdriale DNA-Neusynthese","niederfrequentes Magnetfeld","autoradiographic studie","magnetic field exposure","DNA repair","mitochondrial DNA synthesis","DNA damage"],"languages":["ger"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113656%22"],"render_values":[{"text":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113656%22","href":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113656%22","code":true}]}]},"links":{"outbound_url":"https://publications.rwth-aachen.de/record/51355","outbound_label":"Repository record","outbound_source":"dc:identifier"},"source_record":{"url":"https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A51355","prefix":"oai_dc"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Korr, Hubert"]},{"key":"dc:creator","label":"Author","values":["Füller, Katja"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:coverage","label":"Dc Coverage","values":["DE"]},{"key":"dc:date","label":"Dc Date","values":["2009"]},{"key":"dc:publisher","label":"Institution","values":["Publikationsserver der RWTH Aachen University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-29740"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["info:eu-repo/classification/ddc/610","Magnetfeldeffekt","DNS-Reparatur","DNS-Strangbruch","Autoradiographie","Mitochondriale DNS","Plexus chorioideus","Primärstoffwechsel","Magnetfeld","Tritium","Thymidin","Medizin","UDS","mitochdriale DNA-Neusynthese","niederfrequentes Magnetfeld","autoradiographic studie","magnetic field exposure","DNA repair","mitochondrial DNA synthesis","DNA damage"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ger"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/record/51355","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113656%22"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The aim of this study was to analyse the effects of a continuous exposure of 9 months old mice to a 50 Hz magnetic field (MF) with flux densities of 1 mT, 0,1 mT, or 0 mT (controls) on specific brain cells (epithelial cells of the choroids plexus of the II. and IV. Ventricle, granule cells of the hippocampal dentate gyrus and cortical endothelial cells. This was carried out by analysing autoradiographs after injection of ³H-thymidine (TdR) concerning nuclear (n) unscheduled DNA synthesis (UDS) and mitochondrial (mt) DNA synthesis in order to obtain information on MF induced nDNA damage and its repair as well as the cell metabolism. 80 male mice (NMRI strain) were randomly divided into four groups, In group I and II, the MF exposure was 1 mT (I) or 0.1 mT (II). Group III was the control group, i.e. sham-exposed with 0 mT. After the end of the MF-exposure or sham-exposure, the mice were injected with ³H-TdR, either after 5 minutes (Group A), or 24 hours (Group B) or 7 days (Group C). After paraffin embedding 3 µm thick paramedian, sagittal sections were cut, and subsequently autoradiographs prepared (exposure time: 250 d at 4°C). 100 consecutive cells per cell type and animal were analysed concerning nuclear and cytoplasmic grain numbers as well as the corresponding profile areas. For background correction cells of six mice without MF-exposure and without ³H-TdR injection were examined additionally. The statistical analyses included univariance analyses and subsequent post-hoc t-tests with so-called Bonferroni-corrections. Furthermore, the new data and those of a preceding pilot study (Schmitz et al., 2004) were combined in order to carry out regression analyses. Regarding the effects of the different MF doses on UDS, only the epithelial cells of the choroids plexus (of both ventricles) showed significant results. Surprisingly, UDS was significantly (p<0.05) smaller after exposure to 1 mT than 0 mT. This was probably caused by effects unrelated to the MF-exposure. In contrast, the results of linear and quadratic regression analyses led suppose that only MF-exposure with more than 1 mT leads to DNA damage in the epithelial cells of the choroids plexus. However, no harmful effects of MF-exposure could be detected on the hippocampal granule cells. The cytoplasmic labelling of the epithelial cells of the choroids plexus also showed a significant decline between 0 mT and 1 mT MF-exposure. Since the data obtained showed a good correlation with the quadratic regression model, it can be assumed that – as in the case of UDS – MF-exposure with a dose higher than 1 mT leads to an increased cellular metabolism."]},{"key":"dc:source","label":"Dc Source","values":["Aachen : Publikationsserver der RWTH Aachen University 107 S. : Ill., graph. Darst. (2009). = Aachen, Techn. Hochsch., Diss., 2009"]},{"key":"dc:title","label":"Title","values":["Autoradiographische Untersuchungen zum Einfluss einer achtwöchigen 50-Hz-Magnetfeld-Exposition auf DNA-Reparatur und mitochondriale DNA-Synthese verschiedener Zellarten im Gehirn adulter Mäuse"]}]}],"canonical_facts":{"dc:contributor":["Korr, Hubert"],"dc:coverage":["DE"],"dc:creator":["Füller, Katja"],"dc:date":["2009"],"dc:description":["The aim of this study was to analyse the effects of a continuous exposure of 9 months old mice to a 50 Hz magnetic field (MF) with flux densities of 1 mT, 0,1 mT, or 0 mT (controls) on specific brain cells (epithelial cells of the choroids plexus of the II. and IV. Ventricle, granule cells of the hippocampal dentate gyrus and cortical endothelial cells. This was carried out by analysing autoradiographs after injection of ³H-thymidine (TdR) concerning nuclear (n) unscheduled DNA synthesis (UDS) and mitochondrial (mt) DNA synthesis in order to obtain information on MF induced nDNA damage and its repair as well as the cell metabolism. 80 male mice (NMRI strain) were randomly divided into four groups, In group I and II, the MF exposure was 1 mT (I) or 0.1 mT (II). Group III was the control group, i.e. sham-exposed with 0 mT. After the end of the MF-exposure or sham-exposure, the mice were injected with ³H-TdR, either after 5 minutes (Group A), or 24 hours (Group B) or 7 days (Group C). After paraffin embedding 3 µm thick paramedian, sagittal sections were cut, and subsequently autoradiographs prepared (exposure time: 250 d at 4°C). 100 consecutive cells per cell type and animal were analysed concerning nuclear and cytoplasmic grain numbers as well as the corresponding profile areas. For background correction cells of six mice without MF-exposure and without ³H-TdR injection were examined additionally. The statistical analyses included univariance analyses and subsequent post-hoc t-tests with so-called Bonferroni-corrections. Furthermore, the new data and those of a preceding pilot study (Schmitz et al., 2004) were combined in order to carry out regression analyses. Regarding the effects of the different MF doses on UDS, only the epithelial cells of the choroids plexus (of both ventricles) showed significant results. Surprisingly, UDS was significantly (p<0.05) smaller after exposure to 1 mT than 0 mT. This was probably caused by effects unrelated to the MF-exposure. In contrast, the results of linear and quadratic regression analyses led suppose that only MF-exposure with more than 1 mT leads to DNA damage in the epithelial cells of the choroids plexus. However, no harmful effects of MF-exposure could be detected on the hippocampal granule cells. The cytoplasmic labelling of the epithelial cells of the choroids plexus also showed a significant decline between 0 mT and 1 mT MF-exposure. Since the data obtained showed a good correlation with the quadratic regression model, it can be assumed that – as in the case of UDS – MF-exposure with a dose higher than 1 mT leads to an increased cellular metabolism."],"dc:identifier":["https://publications.rwth-aachen.de/record/51355","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113656%22"],"dc:language":["ger"],"dc:publisher":["Publikationsserver der RWTH Aachen University"],"dc:relation":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-29740"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:source":["Aachen : Publikationsserver der RWTH Aachen University 107 S. : Ill., graph. Darst. (2009). = Aachen, Techn. 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