Publikationsserver der RWTH Aachen University
Autoradiographische Modelluntersuchungen an der Maus zum Einfluss einer achtwöchigen 50-Hz-Magnetfeld-Exposition auf DNA-Reparatur und Zellproliferation in der Milz
Abstract
dc:descriptionThe aim of this thesis was to study autoradiographically after injection of ³H-thymidine (TdR) the influence of an 8-week magnetic field (MF) exposure of 50 Hz with 0.1 mT or 1 mT flux density on cell proliferation in the spleen germinal center and nuclear (n) DNA-repair capacity in the mantle zone of follicular spleen cells of adult mice. Altogether 72 male mice were exposed to a 50 Hz magnetic field with flux densities of 0 mT (control), 0.1 mT or 1 mT for a period of eight weeks. In addition, three completely untreated animals were used for determination of autoradiographic background labeling. The mice were injected with ³H-TdR 5 minutes (sub-group A), 24 hours (sub-group B) or seven days (sub-group C) after the end of MF exposure. All mice were sacrificed 115 min after ³H-TdR injection with an intracardial perfusion fixation. 3 µm thick paraffin sections were deparaffinized, Feulgen stained, and autoradiographs were prepared using Ilford K2-emulsion and the dipping method. The exposition time was 251 days. In order to study cell proliferation in the spleen germinal center, the total number of follicles per section as well as the number of follicles containing so-called clusters, i.e. accumulations of S phase cells, and the number of S-phase cells per 10 follicles were determined. The (relative) extend of nDNA damage of spleen mantle zone cells was calculated by measuring nuclear silver grain numbers. Using cumulative frequency distributions of the silver grains, artifacts (arisen during the production of the autoradiographs) could be recognized; these autoradiographs were excluded from further calculations. The collected data were analyzed using univariance analysis (generalized linear model) and afterwards subjected to post hoc t-tests considering Bonferroni’s correction. For p <0.05 no statistically significant results were found. This indicates that a magnetic field exposure as used had no influence on the cell proliferation in the germinal zone of the spleen and did not lead to (measurable) nDNA damage.
Degree
thesis:*- Grantor dc:publisher
- Publikationsserver der RWTH Aachen University
- Year dc:date
- 2009
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Hüsing, Corinna Elisabeth Gesina
- Contributors dc:contributor
-
- Korr, Hubert
Subjects
dc:subject × 11Rights
dc:rights- Statement dc:rights
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- info:eu-repo/semantics/openAccess
- Language dc:language
- ger