{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:50671"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:50671","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Untersuchungen zum Glutathiontransport und Glutathionstoffwechsel der Spalthefe Schizosaccharomyces pombe","abstract":"Though big areas of the glutathione metabolism (mainly the enzymes of glutathione synthesis) of the fission yeast S. pombe have already been studied in detail, the way S. pombe takes up the tripeptide from the medium was not known up to now. In budding yeast S. cerevisiae the gene product of the ORF YJL212c Hgt1p was identified as a transporter with high affinity for glutathione (Bourbouloux et al., 2000). This transporter is localized within the outer membrane of cells and belongs to the OPT transporter family. OPT transporters differ from the two other oligopeptide transporter classes ABC and PTR. All members of the OPT family contain 12 to 14 transmembral regions, as well as the conserved motif SPYxEVRxxVxxxDDP within their primary sequence. An alignment of the aminoacid sequence of Hgt1p with proteins of the fission yeast revealed two proteins with an overall high homology to Hgt1p. These were ISP4 (SPBC29B5.02c) and SpOPT1 (SPAc29B12.10c). ISP4 had already been characterised by Lubkowitz et al. (1998), who found it to be a tetrapeptide transporter. In contrast SpOPT1 had not been analyzed yet and was thus chosen to be the object of the here presented study. Disruption of the ORF SPAC29B12.10c led to the inability of cells to take up glutathione from the medium and to utilize it as a source of sulfur. This was varified through growth experiments using media that contained glutathione as a sole source of sulfur. Furthermore did the disrupted cells contain almost no detectable glutathione when compared to cells of the wildtype and it was not possible to isolate a strain didrupted in Spopt1 as well as gsh2 after crossing strains containing one of either disruption. All this led to the thought that SpOPT1 has to be a transporter, mediating glutathione uptake. In consistency localization studies showed that SpOPT1 is localized in the outer membrane of the fission yeast. The phenotype of the Spopt1 disrupted strain could be healed by the expression of either Spopt1 and HGT1, whereas the phenotype of the HGT1 disrupted strain was not complemented by Spopt1. Overexpression of Spopt1 in the fission yeast did not lead to an alteration of phenotype, whereas the overexpression of HGT1 in S. pombe resulted in a sensitivity for high concentrations of glutathione. In conclusion the here presented studies identified the protein SpOPT1, expressed by the ORF SPAC29B12.10c, as another member of the OPT transporter family. Furthermore SpOPT1 seems to be the main transporter for glutathione in the fission yeast. In addition some interesting differences between fission and budding yeast were found. Altogether the presented study contributes to the further analysis of the glutathione metabolism of S. pombe.","abstract_html":"Though big areas of the glutathione metabolism (mainly the enzymes of glutathione synthesis) of the fission yeast S. pombe have already been studied in detail, the way S. pombe takes up the tripeptide from the medium was not known up to now. In budding yeast S. cerevisiae the gene product of the ORF YJL212c Hgt1p was identified as a transporter with high affinity for glutathione (Bourbouloux et al., 2000). This transporter is localized within the outer membrane of cells and belongs to the OPT transporter family. OPT transporters differ from the two other oligopeptide transporter classes ABC and PTR. All members of the OPT family contain 12 to 14 transmembral regions, as well as the conserved motif SPYxEVRxxVxxxDDP within their primary sequence. An alignment of the aminoacid sequence of Hgt1p with proteins of the fission yeast revealed two proteins with an overall high homology to Hgt1p. These were ISP4 (SPBC29B5.02c) and SpOPT1 (SPAc29B12.10c). ISP4 had already been characterised by Lubkowitz et al. (1998), who found it to be a tetrapeptide transporter. In contrast SpOPT1 had not been analyzed yet and was thus chosen to be the object of the here presented study. Disruption of the ORF SPAC29B12.10c led to the inability of cells to take up glutathione from the medium and to utilize it as a source of sulfur. This was varified through growth experiments using media that contained glutathione as a sole source of sulfur. Furthermore did the disrupted cells contain almost no detectable glutathione when compared to cells of the wildtype and it was not possible to isolate a strain didrupted in Spopt1 as well as gsh2 after crossing strains containing one of either disruption. All this led to the thought that SpOPT1 has to be a transporter, mediating glutathione uptake. In consistency localization studies showed that SpOPT1 is localized in the outer membrane of the fission yeast. The phenotype of the Spopt1 disrupted strain could be healed by the expression of either Spopt1 and HGT1, whereas the phenotype of the HGT1 disrupted strain was not complemented by Spopt1. Overexpression of Spopt1 in the fission yeast did not lead to an alteration of phenotype, whereas the overexpression of HGT1 in S. pombe resulted in a sensitivity for high concentrations of glutathione. In conclusion the here presented studies identified the protein SpOPT1, expressed by the ORF SPAC29B12.10c, as another member of the OPT transporter family. Furthermore SpOPT1 seems to be the main transporter for glutathione in the fission yeast. In addition some interesting differences between fission and budding yeast were found. Altogether the presented study contributes to the further analysis of the glutathione metabolism of S. pombe.","abstract_has_math":false,"creators":["Dworeck, Tamara"],"institution":"Publikationsserver der RWTH Aachen University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Wolf, Klaus"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2009,"date_issued":"2009","date_published":"2009","updated_at":"2026-07-30T19:40:25Z","subjects":["info:eu-repo/classification/ddc/570","Schizosaccharomyces pombe","Glutathion","Glutathionstoffwechsel","Hefeartige Pilze","Biowissenschaften, Biologie","Glutathiontransport","OPT-Transporter","glutathione","yeasts","transport","OPT transporter"],"languages":["ger"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113206%22"],"render_values":[{"text":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113206%22","href":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113206%22","code":true}]}]},"links":{"outbound_url":"https://publications.rwth-aachen.de/record/50671","outbound_label":"Repository record","outbound_source":"dc:identifier"},"source_record":{"url":"https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A50671","prefix":"oai_dc"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Wolf, Klaus"]},{"key":"dc:creator","label":"Author","values":["Dworeck, Tamara"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:coverage","label":"Dc Coverage","values":["DE"]},{"key":"dc:date","label":"Dc Date","values":["2009"]},{"key":"dc:publisher","label":"Institution","values":["Publikationsserver der RWTH Aachen University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-27431"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["info:eu-repo/classification/ddc/570","Schizosaccharomyces pombe","Glutathion","Glutathionstoffwechsel","Hefeartige Pilze","Biowissenschaften, Biologie","Glutathiontransport","OPT-Transporter","glutathione","yeasts","transport","OPT transporter"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ger"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/record/50671","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113206%22"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Though big areas of the glutathione metabolism (mainly the enzymes of glutathione synthesis) of the fission yeast S. pombe have already been studied in detail, the way S. pombe takes up the tripeptide from the medium was not known up to now. In budding yeast S. cerevisiae the gene product of the ORF YJL212c Hgt1p was identified as a transporter with high affinity for glutathione (Bourbouloux et al., 2000). This transporter is localized within the outer membrane of cells and belongs to the OPT transporter family. OPT transporters differ from the two other oligopeptide transporter classes ABC and PTR. All members of the OPT family contain 12 to 14 transmembral regions, as well as the conserved motif SPYxEVRxxVxxxDDP within their primary sequence. An alignment of the aminoacid sequence of Hgt1p with proteins of the fission yeast revealed two proteins with an overall high homology to Hgt1p. These were ISP4 (SPBC29B5.02c) and SpOPT1 (SPAc29B12.10c). ISP4 had already been characterised by Lubkowitz et al. (1998), who found it to be a tetrapeptide transporter. In contrast SpOPT1 had not been analyzed yet and was thus chosen to be the object of the here presented study. Disruption of the ORF SPAC29B12.10c led to the inability of cells to take up glutathione from the medium and to utilize it as a source of sulfur. This was varified through growth experiments using media that contained glutathione as a sole source of sulfur. Furthermore did the disrupted cells contain almost no detectable glutathione when compared to cells of the wildtype and it was not possible to isolate a strain didrupted in Spopt1 as well as gsh2 after crossing strains containing one of either disruption. All this led to the thought that SpOPT1 has to be a transporter, mediating glutathione uptake. In consistency localization studies showed that SpOPT1 is localized in the outer membrane of the fission yeast. The phenotype of the Spopt1 disrupted strain could be healed by the expression of either Spopt1 and HGT1, whereas the phenotype of the HGT1 disrupted strain was not complemented by Spopt1. Overexpression of Spopt1 in the fission yeast did not lead to an alteration of phenotype, whereas the overexpression of HGT1 in S. pombe resulted in a sensitivity for high concentrations of glutathione. In conclusion the here presented studies identified the protein SpOPT1, expressed by the ORF SPAC29B12.10c, as another member of the OPT transporter family. Furthermore SpOPT1 seems to be the main transporter for glutathione in the fission yeast. In addition some interesting differences between fission and budding yeast were found. Altogether the presented study contributes to the further analysis of the glutathione metabolism of S. pombe."]},{"key":"dc:source","label":"Dc Source","values":["Aachen : Publikationsserver der RWTH Aachen University IX, 132, [11] S.: Ill., graph. Darst. (2009). = Aachen, Techn. Hochsch., Diss., 2009"]},{"key":"dc:title","label":"Title","values":["Untersuchungen zum Glutathiontransport und Glutathionstoffwechsel der Spalthefe Schizosaccharomyces pombe"]}]}],"canonical_facts":{"dc:contributor":["Wolf, Klaus"],"dc:coverage":["DE"],"dc:creator":["Dworeck, Tamara"],"dc:date":["2009"],"dc:description":["Though big areas of the glutathione metabolism (mainly the enzymes of glutathione synthesis) of the fission yeast S. pombe have already been studied in detail, the way S. pombe takes up the tripeptide from the medium was not known up to now. In budding yeast S. cerevisiae the gene product of the ORF YJL212c Hgt1p was identified as a transporter with high affinity for glutathione (Bourbouloux et al., 2000). This transporter is localized within the outer membrane of cells and belongs to the OPT transporter family. OPT transporters differ from the two other oligopeptide transporter classes ABC and PTR. All members of the OPT family contain 12 to 14 transmembral regions, as well as the conserved motif SPYxEVRxxVxxxDDP within their primary sequence. An alignment of the aminoacid sequence of Hgt1p with proteins of the fission yeast revealed two proteins with an overall high homology to Hgt1p. These were ISP4 (SPBC29B5.02c) and SpOPT1 (SPAc29B12.10c). ISP4 had already been characterised by Lubkowitz et al. (1998), who found it to be a tetrapeptide transporter. In contrast SpOPT1 had not been analyzed yet and was thus chosen to be the object of the here presented study. Disruption of the ORF SPAC29B12.10c led to the inability of cells to take up glutathione from the medium and to utilize it as a source of sulfur. This was varified through growth experiments using media that contained glutathione as a sole source of sulfur. Furthermore did the disrupted cells contain almost no detectable glutathione when compared to cells of the wildtype and it was not possible to isolate a strain didrupted in Spopt1 as well as gsh2 after crossing strains containing one of either disruption. All this led to the thought that SpOPT1 has to be a transporter, mediating glutathione uptake. In consistency localization studies showed that SpOPT1 is localized in the outer membrane of the fission yeast. The phenotype of the Spopt1 disrupted strain could be healed by the expression of either Spopt1 and HGT1, whereas the phenotype of the HGT1 disrupted strain was not complemented by Spopt1. Overexpression of Spopt1 in the fission yeast did not lead to an alteration of phenotype, whereas the overexpression of HGT1 in S. pombe resulted in a sensitivity for high concentrations of glutathione. In conclusion the here presented studies identified the protein SpOPT1, expressed by the ORF SPAC29B12.10c, as another member of the OPT transporter family. Furthermore SpOPT1 seems to be the main transporter for glutathione in the fission yeast. In addition some interesting differences between fission and budding yeast were found. Altogether the presented study contributes to the further analysis of the glutathione metabolism of S. pombe."],"dc:identifier":["https://publications.rwth-aachen.de/record/50671","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113206%22"],"dc:language":["ger"],"dc:publisher":["Publikationsserver der RWTH Aachen University"],"dc:relation":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-27431"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:source":["Aachen : Publikationsserver der RWTH Aachen University IX, 132, [11] S.: Ill., graph. Darst. (2009). = Aachen, Techn. Hochsch., Diss., 2009"],"dc:subject":["info:eu-repo/classification/ddc/570","Schizosaccharomyces pombe","Glutathion","Glutathionstoffwechsel","Hefeartige Pilze","Biowissenschaften, Biologie","Glutathiontransport","OPT-Transporter","glutathione","yeasts","transport","OPT transporter"],"dc:title":["Untersuchungen zum Glutathiontransport und Glutathionstoffwechsel der Spalthefe Schizosaccharomyces pombe"],"dc:type":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]},"updated_at":"2026-07-30T19:40:25Z"}