{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:50262"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:50262","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Fasten-induzierte Regulation hepatischer basolateraler Gallensäuretransporter in Ratten vermittelt über PGC-1[alpha]/HNF-4[alpha]","abstract":"Fasting induces numerous adaptive changes in metabolism by several central signaling pathways, the most important represented by the HNF4-alpha PGC-1-alpha-pathway. Because HNF4-alpha has been identified as central regulator of basolateral bile acid transporters and a previous study reports increased basolateral bile acid uptake into the liver during fasting, we hypothesized that HNF4-alpha is involved in fasting-induced bile acid uptake via upregulation of basolateral bile acid transporters. In rats, mRNA of Ntcp, Oatp1, and Oatp2 were significantly increased after 48 h of fasting. Protein expression as determined by Western blot showed significant increases for all three transporters 72 h after the onset of fasting. Whereas binding activity of HNF1-alpha in electrophoretic mobility shift assays remained unchanged, HNF4-alpha binding activity to the Ntcp promoter was increased significantly. In line with this result, we found significantly increased mRNA expression of HNF4-alpha and PGC-1-alpha. We conclude that upregulation of the basolateral bile acid transporters Ntcp, Oatp1, and Oatp2 in fasted rats is mediated via the HNF4-alpha/PGC-1-alpha pathway and that this signalling pathway is not just involved in the regulation of the gluconeogenesis but can be seen as a global adaptive process in the fasting situation.","abstract_html":"Fasting induces numerous adaptive changes in metabolism by several central signaling pathways, the most important represented by the HNF4-alpha PGC-1-alpha-pathway. Because HNF4-alpha has been identified as central regulator of basolateral bile acid transporters and a previous study reports increased basolateral bile acid uptake into the liver during fasting, we hypothesized that HNF4-alpha is involved in fasting-induced bile acid uptake via upregulation of basolateral bile acid transporters. In rats, mRNA of Ntcp, Oatp1, and Oatp2 were significantly increased after 48 h of fasting. Protein expression as determined by Western blot showed significant increases for all three transporters 72 h after the onset of fasting. Whereas binding activity of HNF1-alpha in electrophoretic mobility shift assays remained unchanged, HNF4-alpha binding activity to the Ntcp promoter was increased significantly. In line with this result, we found significantly increased mRNA expression of HNF4-alpha and PGC-1-alpha. We conclude that upregulation of the basolateral bile acid transporters Ntcp, Oatp1, and Oatp2 in fasted rats is mediated via the HNF4-alpha/PGC-1-alpha pathway and that this signalling pathway is not just involved in the regulation of the gluconeogenesis but can be seen as a global adaptive process in the fasting situation.","abstract_has_math":false,"creators":["Porn, Anne Christine"],"institution":"Publikationsserver der RWTH Aachen University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Trautwein, Christian"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2008,"date_issued":"2008","date_published":"2008","updated_at":"2026-07-30T19:40:16Z","subjects":["info:eu-repo/classification/ddc/610","Fasten","Gallensäuren","Enterohepatischer Kreislauf","Medizin","organische Anionentransporter","Ratten","Transkriptionsfaktoren","Fasting","bile acids","transporter","rats","regulation"],"languages":["ger"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-112813%22"],"render_values":[{"text":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-112813%22","href":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-112813%22","code":true}]}]},"links":{"outbound_url":"https://publications.rwth-aachen.de/record/50262","outbound_label":"Repository record","outbound_source":"dc:identifier"},"source_record":{"url":"https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A50262","prefix":"oai_dc"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Trautwein, Christian"]},{"key":"dc:creator","label":"Author","values":["Porn, Anne Christine"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:coverage","label":"Dc Coverage","values":["DE"]},{"key":"dc:date","label":"Dc Date","values":["2008"]},{"key":"dc:publisher","label":"Institution","values":["Publikationsserver der RWTH Aachen University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-24706"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["info:eu-repo/classification/ddc/610","Fasten","Gallensäuren","Enterohepatischer Kreislauf","Medizin","organische Anionentransporter","Ratten","Transkriptionsfaktoren","Fasting","bile acids","transporter","rats","regulation"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ger"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/record/50262","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-112813%22"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Fasting induces numerous adaptive changes in metabolism by several central signaling pathways, the most important represented by the HNF4-alpha PGC-1-alpha-pathway. Because HNF4-alpha has been identified as central regulator of basolateral bile acid transporters and a previous study reports increased basolateral bile acid uptake into the liver during fasting, we hypothesized that HNF4-alpha is involved in fasting-induced bile acid uptake via upregulation of basolateral bile acid transporters. In rats, mRNA of Ntcp, Oatp1, and Oatp2 were significantly increased after 48 h of fasting. Protein expression as determined by Western blot showed significant increases for all three transporters 72 h after the onset of fasting. Whereas binding activity of HNF1-alpha in electrophoretic mobility shift assays remained unchanged, HNF4-alpha binding activity to the Ntcp promoter was increased significantly. In line with this result, we found significantly increased mRNA expression of HNF4-alpha and PGC-1-alpha. We conclude that upregulation of the basolateral bile acid transporters Ntcp, Oatp1, and Oatp2 in fasted rats is mediated via the HNF4-alpha/PGC-1-alpha pathway and that this signalling pathway is not just involved in the regulation of the gluconeogenesis but can be seen as a global adaptive process in the fasting situation."]},{"key":"dc:source","label":"Dc Source","values":["Aachen : Publikationsserver der RWTH Aachen University 65 S. : Ill., graph. Darst. (2008). = Aachen, Techn. Hochsch., Diss., 2008"]},{"key":"dc:title","label":"Title","values":["Fasten-induzierte Regulation hepatischer basolateraler Gallensäuretransporter in Ratten vermittelt über PGC-1[alpha]/HNF-4[alpha]"]}]}],"canonical_facts":{"dc:contributor":["Trautwein, Christian"],"dc:coverage":["DE"],"dc:creator":["Porn, Anne Christine"],"dc:date":["2008"],"dc:description":["Fasting induces numerous adaptive changes in metabolism by several central signaling pathways, the most important represented by the HNF4-alpha PGC-1-alpha-pathway. Because HNF4-alpha has been identified as central regulator of basolateral bile acid transporters and a previous study reports increased basolateral bile acid uptake into the liver during fasting, we hypothesized that HNF4-alpha is involved in fasting-induced bile acid uptake via upregulation of basolateral bile acid transporters. In rats, mRNA of Ntcp, Oatp1, and Oatp2 were significantly increased after 48 h of fasting. Protein expression as determined by Western blot showed significant increases for all three transporters 72 h after the onset of fasting. Whereas binding activity of HNF1-alpha in electrophoretic mobility shift assays remained unchanged, HNF4-alpha binding activity to the Ntcp promoter was increased significantly. In line with this result, we found significantly increased mRNA expression of HNF4-alpha and PGC-1-alpha. We conclude that upregulation of the basolateral bile acid transporters Ntcp, Oatp1, and Oatp2 in fasted rats is mediated via the HNF4-alpha/PGC-1-alpha pathway and that this signalling pathway is not just involved in the regulation of the gluconeogenesis but can be seen as a global adaptive process in the fasting situation."],"dc:identifier":["https://publications.rwth-aachen.de/record/50262","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-112813%22"],"dc:language":["ger"],"dc:publisher":["Publikationsserver der RWTH Aachen University"],"dc:relation":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-24706"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:source":["Aachen : Publikationsserver der RWTH Aachen University 65 S. : Ill., graph. Darst. (2008). = Aachen, Techn. Hochsch., Diss., 2008"],"dc:subject":["info:eu-repo/classification/ddc/610","Fasten","Gallensäuren","Enterohepatischer Kreislauf","Medizin","organische Anionentransporter","Ratten","Transkriptionsfaktoren","Fasting","bile acids","transporter","rats","regulation"],"dc:title":["Fasten-induzierte Regulation hepatischer basolateraler Gallensäuretransporter in Ratten vermittelt über PGC-1[alpha]/HNF-4[alpha]"],"dc:type":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]},"updated_at":"2026-07-30T19:40:16Z"}