{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:50238"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:50238","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Immunomodulation der Kartoffel-Blattrollvirus (PLRV) Multiplikation via Expression virusspezifischer rekombinanter Antikörper in planta","abstract":"The proteolytical activity of the non-structural protein P1 of Potato leafroll virus (PLRV) leads to the formation of a subset of P1 derivates including P1-C25 and the VPg. In order to study the relevance of P1 and/or its derivate P1-C25 to the viral life-cycle the VH- and VL-domains of a monoclonal antibody mAbP1-C25 raised against the carboxy terminus of P1 was cloned in order to obtain a single chain variable fragment scFvP1-C25 that was subsequently analysed. The resulting antibody was bacterially expressed in the single chain format and designated as scFvP1-C25. The engineered scFvP1-C25 displayed identical binding specificity of the parental monoclonal antibody mAbP1-C25 and could be used for immunodetection of recombinant and native P1 and P1-C25. In a transient expression approach co-transformation of an infectious PLRV cDNA clone and the antibody scFvP1-C25 in Nicotiana tabacum strongly reduces virus accumulation. Transgenic potato plants expressing scFvP1-C25 showed high levels of resistance following PLRV inoculation by viruliferous aphids. In conclusion, transient or stable expression of a single chain fragment variable engineered against the carboxy terminus of P1 greatly reduces PLRV accumulation indicating the importance of P1 and/or P1-C25 for the life cycle of PLRV. This is the first report studying PLRV gene function in vivo without genetic alteration of the viral genome and of plantibody-mediated reduction in virus accumulation to a luteovirus.","abstract_html":"The proteolytical activity of the non-structural protein P1 of Potato leafroll virus (PLRV) leads to the formation of a subset of P1 derivates including P1-C25 and the VPg. In order to study the relevance of P1 and/or its derivate P1-C25 to the viral life-cycle the VH- and VL-domains of a monoclonal antibody mAbP1-C25 raised against the carboxy terminus of P1 was cloned in order to obtain a single chain variable fragment scFvP1-C25 that was subsequently analysed. The resulting antibody was bacterially expressed in the single chain format and designated as scFvP1-C25. The engineered scFvP1-C25 displayed identical binding specificity of the parental monoclonal antibody mAbP1-C25 and could be used for immunodetection of recombinant and native P1 and P1-C25. In a transient expression approach co-transformation of an infectious PLRV cDNA clone and the antibody scFvP1-C25 in Nicotiana tabacum strongly reduces virus accumulation. Transgenic potato plants expressing scFvP1-C25 showed high levels of resistance following PLRV inoculation by viruliferous aphids. In conclusion, transient or stable expression of a single chain fragment variable engineered against the carboxy terminus of P1 greatly reduces PLRV accumulation indicating the importance of P1 and/or P1-C25 for the life cycle of PLRV. This is the first report studying PLRV gene function in vivo without genetic alteration of the viral genome and of plantibody-mediated reduction in virus accumulation to a luteovirus.","abstract_has_math":false,"creators":["Nickel, Holger"],"institution":"Publikationsserver der RWTH Aachen University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Fischer, Rainer"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2007,"date_issued":"2007","date_published":"2007","updated_at":"2026-07-30T19:40:16Z","subjects":["info:eu-repo/classification/ddc/570","Biotechnologie","Pflanzenkrankheit","Biowissenschaften, Biologie","molekulare Pflanzenbiotechnologie","biotechnology","molecular plant biotechnology"],"languages":["ger"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-112791%22"],"render_values":[{"text":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-112791%22","href":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-112791%22","code":true}]}]},"links":{"outbound_url":"https://publications.rwth-aachen.de/record/50238","outbound_label":"Repository record","outbound_source":"dc:identifier"},"source_record":{"url":"https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A50238","prefix":"oai_dc"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Fischer, Rainer"]},{"key":"dc:creator","label":"Author","values":["Nickel, Holger"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:coverage","label":"Dc Coverage","values":["DE"]},{"key":"dc:date","label":"Dc Date","values":["2007"]},{"key":"dc:publisher","label":"Institution","values":["Publikationsserver der RWTH Aachen University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-25029"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["info:eu-repo/classification/ddc/570","Biotechnologie","Pflanzenkrankheit","Biowissenschaften, Biologie","molekulare Pflanzenbiotechnologie","biotechnology","molecular plant biotechnology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ger"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/record/50238","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-112791%22"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The proteolytical activity of the non-structural protein P1 of Potato leafroll virus (PLRV) leads to the formation of a subset of P1 derivates including P1-C25 and the VPg. In order to study the relevance of P1 and/or its derivate P1-C25 to the viral life-cycle the VH- and VL-domains of a monoclonal antibody mAbP1-C25 raised against the carboxy terminus of P1 was cloned in order to obtain a single chain variable fragment scFvP1-C25 that was subsequently analysed. The resulting antibody was bacterially expressed in the single chain format and designated as scFvP1-C25. The engineered scFvP1-C25 displayed identical binding specificity of the parental monoclonal antibody mAbP1-C25 and could be used for immunodetection of recombinant and native P1 and P1-C25. In a transient expression approach co-transformation of an infectious PLRV cDNA clone and the antibody scFvP1-C25 in Nicotiana tabacum strongly reduces virus accumulation. Transgenic potato plants expressing scFvP1-C25 showed high levels of resistance following PLRV inoculation by viruliferous aphids. In conclusion, transient or stable expression of a single chain fragment variable engineered against the carboxy terminus of P1 greatly reduces PLRV accumulation indicating the importance of P1 and/or P1-C25 for the life cycle of PLRV. This is the first report studying PLRV gene function in vivo without genetic alteration of the viral genome and of plantibody-mediated reduction in virus accumulation to a luteovirus."]},{"key":"dc:source","label":"Dc Source","values":["Aachen : Publikationsserver der RWTH Aachen University 121 S. : Ill., graph. Darst. (2007). = Aachen, Techn. Hochsch., Diss., 2007"]},{"key":"dc:title","label":"Title","values":["Immunomodulation der Kartoffel-Blattrollvirus (PLRV) Multiplikation via Expression virusspezifischer rekombinanter Antikörper in planta"]}]}],"canonical_facts":{"dc:contributor":["Fischer, Rainer"],"dc:coverage":["DE"],"dc:creator":["Nickel, Holger"],"dc:date":["2007"],"dc:description":["The proteolytical activity of the non-structural protein P1 of Potato leafroll virus (PLRV) leads to the formation of a subset of P1 derivates including P1-C25 and the VPg. In order to study the relevance of P1 and/or its derivate P1-C25 to the viral life-cycle the VH- and VL-domains of a monoclonal antibody mAbP1-C25 raised against the carboxy terminus of P1 was cloned in order to obtain a single chain variable fragment scFvP1-C25 that was subsequently analysed. The resulting antibody was bacterially expressed in the single chain format and designated as scFvP1-C25. The engineered scFvP1-C25 displayed identical binding specificity of the parental monoclonal antibody mAbP1-C25 and could be used for immunodetection of recombinant and native P1 and P1-C25. In a transient expression approach co-transformation of an infectious PLRV cDNA clone and the antibody scFvP1-C25 in Nicotiana tabacum strongly reduces virus accumulation. Transgenic potato plants expressing scFvP1-C25 showed high levels of resistance following PLRV inoculation by viruliferous aphids. In conclusion, transient or stable expression of a single chain fragment variable engineered against the carboxy terminus of P1 greatly reduces PLRV accumulation indicating the importance of P1 and/or P1-C25 for the life cycle of PLRV. This is the first report studying PLRV gene function in vivo without genetic alteration of the viral genome and of plantibody-mediated reduction in virus accumulation to a luteovirus."],"dc:identifier":["https://publications.rwth-aachen.de/record/50238","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-112791%22"],"dc:language":["ger"],"dc:publisher":["Publikationsserver der RWTH Aachen University"],"dc:relation":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-25029"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:source":["Aachen : Publikationsserver der RWTH Aachen University 121 S. : Ill., graph. Darst. (2007). = Aachen, Techn. Hochsch., Diss., 2007"],"dc:subject":["info:eu-repo/classification/ddc/570","Biotechnologie","Pflanzenkrankheit","Biowissenschaften, Biologie","molekulare Pflanzenbiotechnologie","biotechnology","molecular plant biotechnology"],"dc:title":["Immunomodulation der Kartoffel-Blattrollvirus (PLRV) Multiplikation via Expression virusspezifischer rekombinanter Antikörper in planta"],"dc:type":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]},"updated_at":"2026-07-30T19:40:16Z"}