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Showing 1 to 8 of 8 for “"zymogram"”.
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ELECTROPHORETIC AND ENZYMATIC IDENTIFICATION OF PROTEINS ASSOCIATED WITH THE DEVELOPMENT OF COMPETENCE IN BACILLUS SUBTILIS
<p>A two-dimensional zymogram procedure for the analysis of nucleases is described. Using purified deoxyribonuclease I (bovine pancreas), as little as 10 pg of nuclease can be detected. Isoelectric focusing (IEF) and non-equilibrium pH gradient electrophoresis (NEPHGE) were compared as first …
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Molecular Analysis of a Cellulase/xylanase gene,celD, From the Ruminal Bacterium Ruminococcus Flavefaciens Fd-1
… and endoxylanase activities. CMC and xylan zymograms of protein produced by FD1-1 and separated by non-denaturing PAGE suggest that the endoglucanase and endoxylanase activities reside on the same polypeptide. Transposon mutagenesis using Tn5supF localized the celD gene within the insert of …
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Proteoliziniu aktyvumu pasižyminčių fermentų biosintezė /
… performed, but no activity was detected in the zymogram. In order to better understand the action of keratinases and expand the application of these enzymes, scientific research in this direction is necessary.
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Expression of Epidermal Growth Factor Receptor in hypopharyngeal cancer and Effect of EGF in Progression of hypopharyngeal cancer
… expression and its activity in the RT-PCR and zymogram analysis. Conclusions: These results suggest that EGF may play an important role in the progression of hypopharyngeal cancer. "
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Cloning and expression of cambialistic Bacteroides fragilis superoxide dismutase gene
… QC1799, produced an active SOD whose activity zymogram was identical to that of purified B. fragilis SOD. In addition, Western analysis of the expressed protein separated on SDS acrylamide gel also displayed a band identical to the subunit of B. fragilis SOD. However, a larger molecular weight …
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Isolation and characterization of starch, starch biosynthetic genes and protease inhibitors from Marama beab (Tylosema Esculentum)
… marama seeds was established using the reverse zymogram technique and fluorogenic substrate N-alpha-benzoyl-l-arginine 7-amido-4-methylcoumarin hydrochloride and cDNA clone encoding a serine protease gene from marama was isolated using newly developed degenerate PCR primers. Native marama starch …
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Investigation of the role of the extracellular β-agarase, produced by the bacterial epiphyte Pseudoalteromonas sp. LS2i, in the virulence response towards the agarophyte Gracilaria gracilis
… weight was estimated to be 39 kDa by SDS-PAGE. Zymogram analysis confirmed that the purified protein is agarolytic and TLC analysis revealed that the predominant end-products of agar hydrolysis are neoagarohexaose and neoagarobiose, which indicates the same mode of action as that observed for …
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A study of the coccoid form and the autolysins of <i>Campylobacter upsaliensis</i>
Conversion of <i>Campylobacter upsaliensis</i> to the nonculturable but viable coccoid form was characterized. Chloramphenicol did not prevent the conversion. Severe decreases in isocitrate dehydrogenase activity and oxygen uptake and extensive degradation of ribosomal RNA suggest that the coccoid …