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Showing 1 to 20 of 26 for “"zymogen"”.
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Altered Morphology And Composition Of Zymogen Granules In Acute Pancreatitis
… secreted following a meal are stored as inactive zymogens within membrane bound secretory vesicles called Zymogen Granules (ZG), and activated extracellularly. In acute pancreatitis however, the digestive enzymes are prematurely activated within the cell, resulting in autodigestion of the tissue. …
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Comparison of the Protein Contents of Bovine Zymogen Granules and of Pancreatic Juice
<p>A zymogen granule fraction has been isolated from bovine pancreas by differential centrifugation under conditions designed to favor homogeneity at the expense of yield. The fraction has been examined in the electron microscope and found to contain a limited amount of contamination represented by …
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The Role of Microtubules in Secretion
… interaction between isolated exocrine pancreatic zymogen granules and brain microtubules has been devised. This system has been studied by electron microscopy, colchicine binding assay for the microtubule protein tubulin and radioimmunoassay. These assays for microtubule proteins have been …
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Exploring and Exploiting Ribonuclease 1: from Protein Biochemistry to Protein Engineering
… as exemplified by RNase S and cyclic RNase-based zymogens, two systems which reversibly attenuate ribonucleolytic activity. In particular, RNase-based zymogens can be activated by exogenous proteases; this schema has biotherapeutic potential, as demonstrated by zymogens which activate in response …
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Une approche afin de produire les différentes conformations de caspase-7 tout en contrôlant l'induction de l'apoptose
… 2 allows the conversion of the enzyme from the zymogen (inactive) state to the active state through a conformation switch that leads to the creation of a substrate binding pocket and the catalytic site. During caspase-7 activation, a 23-residue N-terminal peptide is also cleaved. Consequently, …
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Epithelial Memory of Resolved Inflammation Limits Tissue Damage While Promoting Pancreatic Tumorigenesis
… tissue damage <em>via</em> rapid decrease of zymogen production. We propose that since activating mutations of KRAS maintain an irreversible ADM, they may be beneficial and under strong positive selection in the context of recurrent pancreatitis.</p>
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Role of Thrombin-Activatable Fibrinolysis Inhibitor in Breast Cancer Metastasis and Angiogenesis
… and tumour angiogenesis. TAFI is a plasma zymogen initially known for its role in attenuating fibrinolysis. TAFIa is formed through cleavage by thrombin, plasmin or, by thrombin in complex with thrombomodulin. TAFIa is a carboxypeptidase, cleaving carboxyl terminal lysine residues from …
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Analysis of plasminogen binding to Treponema denticola, a key periopathogen
… is a plasma glycoprotein that circulates as a zymogen. Its active form, plasmin, degrades components of the extracellular matrix and cleaves complement proteins C3b and C5 inhibiting complement pathway progression. Through molecular and biochemical analyses, this study demonstrates that FhbB …
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Oxidative Modifications of Apolipoprotein(a): Implications for Proinflammatory and Prothrombotic Roles of Lipoprotein(a) in the Vasculature
… (CVD). Apo(a) bears striking homology with the zymogen plasminogen and possesses several similar structural features. A key feature shared between these proteins is the presence of multiple repeats of a kringle domain, which possesses the ability to bind to exposed lysine residues with high …
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The tissue factor-factor VII(a) complex in blood coagulation
… membrane. It has been reported that, in vitro, zymogen FVII can be activated to FVIIa via limited proteolysis at a single peptide bond by many proteases, including factor Xa, factor IXa, thrombin, and factor XIIa, as well as FVIIa in complex with TF. By using a FVII mutant (S344A), in which the …
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Discovery Of Inactive E* Conformations In Thrombin And Other Vitamin K- Dependent Clotting Proteases
… many of these proteases, activity unfolds when a zymogen is activated by limited proteolysis and the associated conformational changes result in the formation of a proper active site and oxyanion hole, both of which are required for efficient hydrolysis of peptide bonds. The transition from …
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The Role of Munc18 Proteins in Physiologic and Pathologic Exocytoses in the Pancreatic Acinar Cell
… is mis-targeting and deregulated fusion of zymogen granules with lysosomal bodies in the acinar cells. This derangement results in premature activation of proteolytic zymogens and autophagic digestion of cellular contents. I have hypothesized an alternate mechanism, which is pathologic …
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Synthetic, Sulfated, Lignin-Based Anticoagulants
… is possibly converting the enzyme back into the zymogen-like shape. In general, the synthetic sulfated lignins can act as a useful foundation to develop anticoagulant, antiplatelet, and anti-inflammatory molecules in the future.</p>
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Interactions of Francisella Tularensis With Components of the Host Fibrinolytic System
… host proteins.</p> <p>Plasminogen (PLG) is a zymogen that upon activation is converted into the serine protease plasmin, which plays a key role in the proteolytic degradation of fibrin blood clots. Many pathogenic organisms have taken advantage of both the broad-specificity and abundance of …
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Studies on cathepsin B of Eimeria tenella and pyroglutamyl peptidase of Leishmania major
… host cells, was expressed as a soluble inactive zymogen in E. coli. Recombinant cathepsin B of E. tenella was functionally expressed as a mature enzyme in the Pichia pastoris inducible system as a glycosylation protein, but the glycosylations did not apparently affect the enzyme’s activity. The …
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Wegener'sche Granulomatose
… (NE)-defizienten Mäusen ein mPR3-Zymogen rekombinant in E. coli als Einschlußkörpermaterial (IB, inclusion bodies) hergestellt. Nach Renaturierung des IB-Materials in vitro wurde mit der Dipeptidylaminopeptidase Cathepsin C das N-terminale Propeptid abgespalten. Das gewonnene Enzym …
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Structural And Functional Studies On Thrombin Allostery
… and unique structural and dynamic changes. A zymogen form of thrombin, prethrombin-2, displays unique structural signatures when compared to α-thrombin. 19F-NMR results corroborate our recent crystallographic data, expand the repertoire obtained from previous x-ray structural studies and …
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Expression of Human Protein C in Transgenic Tobacco
… anticoagulant pathway. Upon activation of the zymogen by thrombin at the endothelial cell surface, the active form of hPC has anticoagulant activity in hemostasis due to its ability to inactivate factors Va and VIIIa. For biological activity, hPC requires several post-translational …
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The daily rhythm of Paneth cells in <i>Mus musculus </i>
… <p>Although such lines are not visible in zymogen granules of the pancreatic exocrine cells, Rothmans group (S. Rothman 1971, Burwen and S. Rothman 1972, S. Rothman <em>et al</em> 1974) reports that pancreatic enzymes are attached to specific sites on large molecules that are themselves …
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