Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"zona pellucida"”.
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In vitro Expression of Human Zona Pellucida Protein 3
<p>Human zona pellucida protein 3 (hZP3) is the putative receptor on the zona pellucida of the mature oocyte that recognizes and binds sperm, and therefore plays a critical role in fertilization.</p> <p>A full length cDNA of hZP3 (1278 bp) was amplified from the human ovary mRNA by reverse …
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Expression, Isolation and Purification of Human Zona Pellucida Protein 3
… sperm surface receptors with the ligands of the zona pellucida, an extracellular matrix surrounding the ovulated oocytes. In humans, the zona pellucida is composed of three major glycoproteins. One of them, ZP3, participates in the primary sperm binding and in the subsequent triggering of the …
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The Blood-Oocyte Barrier: Morphological Evidence Implicating The Zona Pellucida
… maternal system. This study proposes that the zona pellucida of the ovarian follicle is the barrier between the immune system and the oocyte. The suggestion is made that the oocyte may play a role in blocking the immune system during its maturation, and follicles in which the oocyte is …
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Characterization of the Biological Functions of Human Recombinant Zona Pellucida Protein 3
<p>Recombinant human zona pellucida protein 3 (rhZP3), expressed. isolated and purified from PA-1 cells, is characterized for its biological activity and the role in the signal transduction pathway. Characterization of the biological activity of rhZP3 was detected by hemizona assay and …
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Phage Display to Identify Peptides Binding to or Penetrating the Mouse Zona Pellucida
The objective of this study was to identify peptide ligands, using phage display techniques, which bind sites on mouse embryos, ovaries, cytoplasmic membranes and/or intracytoplasmic components. Specifically, M13 coliphage 7-mer, 12-mer and 15-mer random peptide libraries were used separately for …
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Sex Chromosome Ratios and Aneuploidy Rates in the Zona Pellucida Selected Spermatozoa From Normal and Subfertile Men
<p>The human hemizona has been demonstrated to select spermatozoa with good motility, normal morphology, and the capacity to undergo the zona-induced acrosomal reaction. The studies conducted here are directed at using the human hemizona to investigate two key questions: (1) whether human …
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Characterization of the Biological Activities of Recombinant Fusion Protein Green Fluorescent Protein/Human Zona Pellucida Protein 3 (GFP/HZP3)
… human sperm through indirect evidence from hemizona assay (HZA).</p> <p>This present study focused on the production of recombinant ZP3 with direct binding activity with human sperm. Through the application of a pEGFP expression vector, fusion protein GFP/ZP3 was successfully generated and …
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Biophysical and biochemical properties of the mammalian male germ cell specific sulfogalactosylglycerolipid (SGG): Contribution to the structure and zona pellucida affinity of pig sperm raft membranes
… the ability to bind specifically to homologous zonae pellucidae (ZP). This binding was dependent on pig ZP3alpha sulfoglycoprotein, a major player in intact sperm binding. The male germ cell specific sulfogalactosylglycerolipid (SGG) was a sperm raft component and participated in sperm raft-ZP …
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Immunoreactivity of anti pZP antibodies from the serum of SpayVac vaccinated mares to equine zona protein
Immunocontraception with the porcine zona pellucida (pZP) antigen is a well-published means of wild horse contraception. There are three pZP vaccines currently proposed for use in horses, Zonastat-H®, PZP-22, and SpayVac®. Despite abundant research concerning the safety and contraceptive efficacy …
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Identifikation antigener Determinanten des ZPB2 Proteins der Hauskatze und Charakterisierung ihrer kontrazeptiven und immunogenen Eigenschaften
Die immunologische Kontrazeption mittels Zona pellucida (ZP) Proteinen gilt als vielversprechender Ansatz für die Reproduktionskontrolle verwilderter Haus- und Wildtierbestände. Da die Applikation von nativer ZP mit Nebenwirkungen verbunden ist, wird die Verwendung einzelner ZP Peptide als …
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Evaluation of the elephant immunocontraception program at Hluhluwe-iMfolozi Park, South Africa
… with over 700 elephants, started a porcine zona pellucida (pZP) immunocontraception program in 2014 with the aim of reducing the population growth rate from 7.7% (2014-2016) to between 2 and 3%, but the program has not yet been formally assessed. In this study, I aimed to (1) evaluate the …
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Factors affecting the relative fertility of males determined by heterospermic insemination in vivo and in vitro
… and these sperm were mixed and added to either zona pellucida-intact or zona free eggs in vitro or inseminated in vivo. Competing sperm from ICR and B6D2F1 males demonstrated that ICR males had a greater proportion of fertilizing sperm in vitro than did B6D2F1 males and was not affected by …
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Arachidonic acid metabolism by early ovine embryos and the role of prostaglandins in one aspect of embryonic development
… have a role in embryo hatching from the zona pellucida. Ewes were superovulated and bred artificially, and embryos were collected on d 7 of pregnancy. Embryos were incubated with ethanol (control), indomethacin, PGE₂ or indomethacin and PGE₂ in an atmosphere of 5% CO₂ and 95% air at 37°C …
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Investigating the effect of phosphodiesterase inhibitors on human sperm motility and function
… This includes enabling sperm to bind to the zona-pellucida of human oocytes, a technique that is closely correlated with predicting IVF outcome. This study investigates the effect of various PDE inhibitors on human sperm motility of samples from both fertile and sub-fertile men. This is done …
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Molecular Interactions During Fertilization: Sperm -Egg Binding and Regulation of the Acrosome Reaction
… the primary sperm receptor for the porcine zona pellucida. Zona proteins were labeled with a fluorescent dye and incubated with live boar sperm. Zona proteins bound the anterior portion of the head, concentrated at the acrosomal ridge. Zona protein binding ability was acquired in the corpus …
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Development of mouse morulae after encapsulation in alginate microgels or poly-l-lysine microcapsule
… to evaluate in vitro and in vivo development of zona pellucid a-intact (ZPI) and zona pellucida-free (ZPF) mouse embryos after encapsulation in either 2% sodium alginate or 0.1% poly-L-Iysine (PLL). In Experiment 1, rate of development of ZPI embryos (n = 150) from morulae to hatched blastocysts …
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Effects of iloprost on embryo development in vitro
… development, using embryo hatching from the zona pellucida as the end point. Results suggest Iloprost was able to reduce the adverse effects of YM-155 on embryo development supporting the theory it activates the survivin pathway.
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Role of Cuzd1 in mammary gland development and tumorigenesis
… are unclear. We have identified the CUB and zona pellucida-like domain containing protein-1 (CUZD1) as a target of estrogen regulation in the mouse mammary epithelium. Mice lacking Cuzd1 exhibited a striking impairment in steroid-induced proliferation of mammary ductal and alveolar …
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Hyperactivated motility in human sperm: regulation, function and significance in IVF
… of the cumulus oophorus and penetration of the zona pellucida. The majority of evidence for the requirement for mammalian hyperactivation has emerged from studies in the mouse and the hamster, however a number of clinical studies in humans have also suggested that its expression is linked to …
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