Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"zinc-binding"”.
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Synthesis of activity-based protein profiling probes for malaria and hypertension disease models & potential novel ACE inhibitors with an attenuated zinc binding group
… (ACE) and P. falciparum A M1 (PfA-M1) are both zinc metalloproteases implicated in hypertension and malaria, respectively. Hypertension affects approximately 26 % of the world’s population while each year over 300 million cases of malaria occur worldwide resulting in between 1.5 and 2.7 million …
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A four-cysteine zinc finger in carboxyltransferase structurally links the functions of enzymatic activity and negative feedback regulation of translation
… crystal structure of the β subunit revealed a zinc-binding domain, a feature common among nucleic acid-binding proteins. Carboxyltransferase preferentially binds mRNA coding for its two subunits over other nucleic acids, suggesting a means by which the enzyme can regulate its own expression. In …
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Experimental and Computational Dynamics of an Aminoacyl-tRNA Synthetase System
… β-linker, which is unique to MetRS species and a zinc binding knuckle. The length of the β-linker was altered to determine how it is important for structuring the zinc binding knuckle. Eight prolines are found in the CP domain which were individually mutated to alanine. Circular dichroism, binding …
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Functional significance of unique sequences in Mycobaterium smegmatis Ku proteins
… a lysine-rich extension at the C-terminus and a zinc-binding motif in the DNA-binding bridge-region. The unique C-terminal tail of M. smegmatis Ku contains several lysine-rich low-complexity PAKKA repeats that are absent from homologs encoded by obligate parasitic mycobacteria, but present in …
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Expression, Purification and Molecular Characterisation of Sideroflexin Proteins
… The results show that human sideroflexin 5 binds zinc ions, which was confirmed subsequently by native mass spectrometry and elemental mass spectrometry. Following this result, the groundwork was laid to find the zinc binding site by combining scanning alanine mutagenesis with thermostability …
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Biomimetics and Host-Guest Chemistry
… tetrahedrally coordinated, catalytically active zinc center, three families of tris(2-pyridyl)methanol derivatives were synthesized and characterized. Zinc binding studies revealed that the binding behaviors of the ligands depended on the steric and electronic properties of the substituents on …
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Characterization and Molecular Analysis of Fragilysin: The Bacteroides fragilis Toxin
… deduced amino acid sequence revealed a signature zinc binding consensus motif (His-Glu-Xx-Xxx-His-Xxx-Xxx-Gly-Xxx-Xxx-His/Met) characteristic of metalloproteinases. Sequence analysis showed close identity with metalloproteinases within the zinc-binding and Met-turn regions. Purified fragilysin …
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Interaction Between Two E3 ligases, NEDD8ylated Cullin and HHARI
… type of E3 ubiquitin ligase containing three zinc-binding RING (Really Interesting New Gene) domains, while adopting mechanisms of HECT (Homologous to E6-AP Carboxyl Terminus) for substrate ubiquitination. Most well known RBRs include Parkin and HOIP, which are associated with Parkinson’s …
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Structural and biophysical analysis of Human DNA repair protein CtIP
… with coiled-coil segments flanking a central zinc-binding motif. In combination with previous crystallographic analysis of the N-terminal tetramerisation motif of CtIP, my new structural information allows modelling the overall architecture of the CtIP NTD. I have explored the ability of CtIP …
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Fluorescent chemosensors for exploring zinc metalloneurochemistry and detecting mercury in aqueous solution
Chapter 1. An Introduction to Zinc Metalloneurochemistry and Zinc Detection in Biology. This chapter presents an overview of zinc neurophysiology and pathology, which provides motivation for the design of new tools and tactics for zinc detection in vivo. A historical account of biological zinc …
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The effect of dietary zinc level upon the efficiency of vitellogenin synthesis by male quail
Manipulation of dietary zinc produced a different body zinc status in male Japanese quail during 3 weeks of treating period. After intramuscular injection with 8μmol/100g body weight of estradiol-17β, Japanese quail were sacrificed and vitellogenin (phosphoprotein) production was assessed in these …
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Phosphorylation of Glucocorticoid Receptor tau1c Transactivation Domain Enhances Binding to CREB Binding Protein (CBP) TAZ2 and a Microfluidic, High Throughput Protein Crystal Growth Method for Microgravity
… rich fragment (CH3) of the co-activator CREB binding protein (CBP). Because CH3 overlaps the CBP transcriptional adaptor zinc binding (TAZ) 2 domain, implicated in phosphorylation dependent binding to other unstructured transcription factor domains, we investigated if GR interacts with TAZ2 …
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Synthesis of Molecular Baskets and Introduction of Inward Facing Functionality
… preorganized host molecules were synthesized. Binding studies of the two hosts with a variety of guests in three solvents demonstrated that an important driving force in the association was the formation of C-H???X-R hydrogen bonds (X = halogen). A deuterated host was utilized to further …
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Molekulare Ursachen des Mohr-Tranebjaerg-Syndromes
… belong to a family of evolutionary conserved zinc finger proteins that are organised in hetero-oligomeric complexes in the mitochondrial intermembrane space (IMS). The TIM8-13 complex assists the import of Tim23, the major component of the translocase for matrix-targeted proteins. Mutations in …
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Mechanistic Investigations of Cyclase Enzymes Involved in Lantibiotic Biosynthesis
… respectively, contain stoichiometric amounts of zinc, implying a possible catalytic role. EXAFS analysis of SpaC suggests that two cysteines coordinate to the metal and possibly two histidines. The metal may function to activate the cysteine thiol of the substrate toward intramolecular Michael …
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Synthetic strategies for potential trypanocides
… These showed weak inhibition of the enzyme. Zinc-binding fragments were screened, and small molecule inhibitors based on salicylhydroxamic acid were then synthesized. These compounds showed modest inhibition, but the excellent ligand efficiency of salicylhydroxamic acid indicates this may be …
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Analysis of Zinc Transporters in C. elegans
Zinc is a trace element essential for organisms, and organisms have homeostatic mechanisms to control zinc metabolism. Zinc metabolism is mediated by numerous proteins including zinc transporters, zinc-responsive transcription factors and zinc-binding proteins. Of these proteins, zinc transporters, …
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Analysis of the Clear Plaque Phenotype of the Bacteriophage HK75
… HK75 is inhibited by specific mutations in the zinc binding domain of the host RNA polymerase beta prime subunit. It shares this rare property with bacteriophage HK022 and other phages that use RNA mediated antitermination to promote early gene expression. Recent genomic analysis of HK75 and …
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The Immune Inhibitor A1 Protease of <em>Bacillus Anthracis</em>
… InhA1 activity is dependent on a conserved zinc binding motif, suggesting that zinc availability regulates InhA1 activity. The convergence of these regulatory mechanisms signifies the importance of tight regulation of InhA1 activity, activity that substantially affects how <em>B. …
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Structure and dynamics of membrane proteins from solid-state NMR
… we have studied the oligomeric structure and the zinc-bound coordination geometry of a de novo designed amyloid fibril that catalyzes ester hydrolysis. By measuring the intermolecular contacts, we determined that peptides form parallel-in- register P-sheets and further assemble into stacked …
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