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Showing 1 to 20 of 217 for “"zinc finger"”.
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The Design and Development of Artificial Zinc Finger Transcription Factors and Zinc Finger Nucleases to the hTERT Locus
… work, we describe the engineering of artificial zinc finger transcription factors (ZFTFs) and zinc finger nucleases (ZFNs) to target sequences at the hTERT promoter. We first explored expansions to the repertoire of sites that can be targeted by ZFNs and modifications of ZFN architecture to …
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Selection studies of zinc finger-DNA recognition
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1997.
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Optimizing Zinc Finger Nucleases for Use in Mammalian Cells
… a double strand break within the target locus. Zinc Finger Nucleases (ZFNs) are designed proteins that fuse a zinc finger DNA binding domain to the nuclease domain from the FokI restriction endonuclease and have been used to induce double strand breaks at precise sequences. Although ZFNs have …
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Studies of the MYM-type zinc finger protein ZMYM3
… a transcription factor, and ZMYM3, an MYM-type zinc finger protein of unknown function. By making several truncation mutants of ZMYM3, I show that a C-terminal region containing a ‘PXP’ repeat motif mediates its interaction with RNase H2, and that the PXP-containing proteins ZMYM2 and ZMYM4 …
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Structural and biochemical studies of zinc finger-DNA complexes
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1998.
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Deep vertebrate roots for mammalian krab zinc-finger transcription factors
KRAB-associated C2H2 zinc-finger (KRAB-ZNF) proteins are the products of a rapidly evolving gene family that traces back to early tetrapods, but which has expanded dramatically to generate an unprecedented level of species-specific diversity. While most attention has been focused on the more …
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Platinum Complexes and Zinc Finger Proteins: From Target Recognition to Fixation
… dinuclear platinum complexes and the C-terminal zinc finger (ZF). The trans complex reacts at a faster rate than the cis isomer and causes N- terminal specific cleavage of the ZF. The dinuclear structure plays a critical role in the peptide cleavage as studies with transplatin (the mononuclear …
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Characterisation of the non-canonical zinc finger protein ZFP263 in mouse
… influence this in human and mouse is the KRAB zinc finger protein (KZFP) family. KZFPs are thought to have rapidly evolved alongside transposable elements and be mediators of transcriptional repression. The few KZFPs that have been characterised so far have been shown to be involved in a wide …
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A strategy for selecting zinc finger proteins for desired DNA sequences
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1997.
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Uncovering the Roles of Human C2H2-Zinc Finger Protein ZNF121 in Gene Regulation
… regulatory effects, and many DNA-binding C2H2-zinc finger proteins (ZFPs) have recently been shown to be RBPs with roles in mRNA biology. I identified C2H2-ZFP, ZNF121, as a YTHDF2 binding partner in affinity purification and mass-spectrometry (AP-MS) experiments and I hypothesized that, like …
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The Study of Au(III) Compounds and their Interaction with Zinc Finger Proteins
… Au(III) compounds designed to selectively target zinc finger (ZF) proteins. ZF proteins exhibit a variety of functions, including transcription, DNA repair, and apoptosis. Displacement of the central zinc ion, along with mutation of coordinated amino acids can result in a loss of biological …
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Tuning DNA binding and gene expression using zinc finger proteins and engineered promoters
… in gene expression. To accomplish this, we used zinc finger proteins (ZFPs) as regulators of engineered promoters assayed by green fluorescent protein (GFP) as a fluorescent transcriptional reporter probe. In particular, we designed strong-binding three finger ZFPs as proof-of-principle …
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Protein-DNA Recognition Models for the Homeodomain and C2H2 Zinc Finger Transcription Factor Families
Transcription factors: TFs) play a central role in the gene regulatory network of each cell. They can stimulate or inhibit transcription of their target genes by binding to short, degenerate DNA sequence motifs. The goal of this research is to build improved models of TF binding site recognition. …
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The Expression of the Zinc Finger Transcription Factor zDC Defines the Classical Dendritic Cell Lineage
… cDCspecific genes identified an uncharacterized zinc finger transcription factor that I call zDC (Zbtb46, Btbd4). This factor is expressed by cDCs but not other myeloid or lymphoid immune populations. The characterization of zDC expression and function involved three major directions. (1) To …
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ZFP36L3: a Unique Member of the Tristetraprolin Family of RNA-Binding Tandem Zinc Finger Proteins
… the tristetraprolin (TTP) family of CCCH tandem zinc finger proteins bind to AU-rich elements in the 3' untranslated regions of certain cellular mRNAs, leading to their deadenylation and destabilization. Studies in knockout mice have demonstrated roles for three of the family members, TTP, …
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ASSESSING MUTATIONS IN A ZINC FINGER DOMAIN OF NF-κB ESSENTIAL MODULATOR (NEMO) USING MOLECULAR DYNAMICS SIMULATIONS
Molecular dynamics (MD) simulations permit the probing of biomolecular systems in
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Zinc Finger Protein 36L1 (ZFP36L1): Gene Expression, Regulation and Interactions with Immune Receptors in Human Tumour Cells.
The Zinc finger protein 36 like 1 (ZFP36L1) or Butyrate response factor 1 (BRF1) is involved in the differentiation, proliferation, and angiogenesis of various cell types. ZFP36L1 can positively or negatively regulate cellular processes such as transcription and signalling, depending on external …
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The KRAB Zinc Finger Protein ZKSCAN3 binds to a SINE-embedded regulatory sequence to repress enhancer activity
KRAB Zinc Finger Proteins (KZFPs) are the largest family of transcription factors in mammals and are generally associated with the binding and repression of transposable elements. KZFPs use their KRAB domain to recruit the cofactor KAP1, which acts as a scaffold for the induction of …
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Primate-specific KRAB zinc finger proteins and their targets rewire regulatory networks related to inflammation and pancreas development
… subfamily of DNA binding factors in human, KRAB Zinc Finger proteins (KZFPs), leads to the epigenetic silencing of their bound loci and have been recently shown to mainly target repetitive regions of the genome, mostly TEs. Interestingly most TEs targeted by KZFPs have lost their ability to …
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The Influence of DNA Methylation on Both H2A.Z Nucleosome Dynamics and the Zinc Finger and Homeoboxes Transcription Factor Family
… proteins, the histone variant H2A.Z and the Zinc Finger and Homeoboxes (ZHX) family of transcription factors. DNA methylation and H2A.Z preferentially occupy exclusive areas of the genome, biasing against their colocalization in the same genomic space. However, exactly how this mutual …
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