Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"zebrafish model"”.
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A zebrafish model to study the schistosome egg granuloma
… life cycle. Here, I have developed a larval zebrafish model to study the formation of the schistosome egg granuloma in detail within an optically transparent animal. I have developed the tools and techniques for implantation of individual Schistosoma mansoni eggs into zebrafish, followed by …
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Rod Photoreceptor Regeneration in a Zebrafish Model with Retinitis Pigmentosa
… until one is completely blind. Unlike mammalian models, Zebrafish have the ability to naturally regenerate their neurons after injury or disease is detected. We have generated a zebrafish model with the most common autosomal dominant form of the inherited retinal degenerative disease known as …
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Investigating non-canonical vertebral development in the zebrafish model system
… chordacentrum mineralisation and patterning in zebrafish and Atlantic salmon, though the question of how the overtly unsegmented notochord could direct segmental mineralisation still remains. My project first aims to address this dual mechanism in the zebrafish model, by testing whether the …
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ANALYSIS OF AXONAL TRANSPORT DEFECTS IN A ZEBRAFISH MODEL FOR SPINAL MUSCULAR ATROPHY
Spinal muscular atrophy (SMA) is an autosomal recessive hereditary neurodegenerative disease caused by a deficiency of the survival motor neuron (SMN) protein. It is characterised by the progressive loss of α-motor neurons (MNs), leading to muscle weakness, atrophy, and in the worst cases death. …
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The Effects of Δ9-tetrahydrocannabinol (THC) on development and hyperekplexia in embryonic zebrafish model
… well. This study demonstrates that in embryonic zebrafish, THC does not detrimentally affect the development of the synapses needed to complete the startle response. Additionally, this study supports the use of THC as a treatment option for the alleviation of spasticity caused by hyperekplexia. …
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The Role of Autophagy in Early Development and Tumor Suppression Using a Zebrafish Model System
… genetically manipulated autophagy-deficient models, important roles for autophagy in development and tumorigenesis have been suggested. Genetic analyses indicate that autophagy is essential for eukaryotic differentiation and development. However, little is known about whether autophagy …
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Oncogenic mutation of GNAQ/11 disrupts melanocyte biology in a Zebrafish model of uveal melanoma
… used Tol-mediated transgenesis to develop a zebrafish model of UM driven by melanocyte specific expression of human oncogenic GNAQ/11Q 209L alleles. In cooperation with tp53M214K/M214K mutation, these GNAQ/11Q 209L transgenic zebrafish develop both uveal and cutaneous melanomas with nearly …
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Investigating the Biological Mechanisms Underlying Axis Morphogenesis Using a Novel Zebrafish Model of Idiopathic-like Scoliosis
… as the historical lack of appropriate animal models, treatment options for AIS patients are limited to bracing and surgery. The Ciruna Lab generated the first genetically defined developmental model of idiopathic-like scoliosis (ptk7a mutant zebrafish) and has characterized a number of …
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Investigating CDK9 inhibitor treatment during the innate inflammatory and regenerative response in a zebrafish model of cardiac injury
… are unknown. Unlike adult mammalian hearts, zebrafish hearts regenerate following injury via cardiomyocyte proliferation. Prolonged neutrophil retention has been shown to impair cardiomyocyte proliferation and myocardial wound regression following cardiac injury in zebrafish. I therefore …
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Steps towards successful cryopreservation of fish gametes: improving laboratory protocols in the Zebrafish model to increase gamete viability and efficiency
… cryopreservation, I outline two experiment using zebrafish (Danio rerio) as a model for freshwater fish species. First, I attempted to extend working time in both eggs and sperm by incubating the gametes in rainbow trout oviductal fluid (RTOF), RTOF + HEPES, or RTOF under a blood-gas atmosphere. …
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Investigating the Impact of Metabolic Dysregulation on Nervous System Development and Function: A Study of CPT2 Deficiency in a Zebrafish Model System
… with schizophrenia. To address the hypothesis, a zebrafish CPT2 deficient model system was developed to investigate CPT2 deficiency during early vertebrate development. Two knockdown morpholino oligonucleotides (MO), a translation blocker (TB) and a splice blocker (SB), were used to reduce CPT2 …
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In vivo identification of drug therapeutics for nemaline myopathy
… pathological features of NM using a klhl41b-/- zebrafish model, a well-established representation of human NM pathology. 1320 FDA-approved drugs were screened using a novel drug repurposing strategy on the klhl41b-/- zebrafish model, an ortholog sharing 80% similarity with KLHL41. Core …
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ZEBRAFISH (DANIO RERIO) AS A MODEL SYSTEM FOR THE FUNCTIONALVALIDATION OF CANDIDATE GENES FOR HUMAN DISEASES
In recent years, zebrafish (Danio rerio) has become increasingly utilized as a model for studying human gene functions and associated diseases. This has been possible due to the very high conservation of genes and of organs and systems which are fully functional within the very first days of …
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Screening of radioprotective agents using zebrafish and Therapeutic effects and protective mechanism of radioprotective candidates on radiation-induced oral mucositis
Screening of radioprotective agents using zebrafish and Therapeutic effects and protective mechanism of radioprotective candidates on radiation-induced oral mucositis Radiation-induced oral mucositis is a dose-limiting toxic side effect for patients with head and neck cancer. About 50–90% of cancer …
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Biochemical changes in animal models of fetal alcohol spectrum disorder
… modified in the chronic ethanol exposure zebrafish model of FASD. Many of these pathways agree with existing literature, but the comprehensive nature of this study expands upon several of them, and links branches that were previously considered in isolation as mediators of FASD. In …
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A Mutation in Alk6b Causes Impaired Germ Cell Differentation and Testicular Germ Cell Tumors in Zebrafish
… infertility and kidney damage. We identified a zebrafish mutant line with a high incidence of GCT during a forward genetic screen to identify cancer susceptibility loci. Homozygous adult males develop tumors consisting of undifferentiated spermatogonia by 4 months of age while heterozygous males …
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Establishment of MITF and TAZ as major determinants of uveal melanoma
… as a tumor suppressor in an oncogenic GNAQ/11 UM zebrafish model. Moreover, we show that resulting MITF-deficient tumors are more de-differentiated and down-regulate PLCβ4-MAPK signaling, but retain active YAP. Furthermore, we establish that YAP, and not PLCβ4, is sufficient to drive MITF-wildtype …
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Mechanisms of Edema Clearance: The Central Role of Lymphatic Vessels and LYVE1 in Fluid Homeostasis
… clearance. In this study, we established a novel zebrafish model of edema. We utilized its optical transparency and intravital imaging capabilities to characterize the dynamic lymphatic responses during edema formation and resolution. Using this platform, we identified key structural and molecular …
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A GNAQ/11-driven zebrafish cancer model identifies MITF and YAP as key determinants for uveal melanoma
… GNAQ or GNA11 (>80% of patients). We developed a zebrafish model of UM by expressing GNAQ[superscript Q209L] in the melanocytes of p53-/- zebrafish. In this model, oncogenic GNAQ activates biological programs involved in pigmentation and tumorigenesis. The melanocyte lineage transcription factor …
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