Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"zebrafish embryo"”.
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Mechanisms of Copper Deficiency in the Zebrafish Embryo
Proper maternal nutrition is critical for early embryonic development. Despite overwhelming epidemiologic data indicating the benefits nutrient supplementation for the developing organism we do not fully understand the genetics of predisposition to abnormal developmental phenotypes when faced with …
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Transient CaMK-II Activation is Necessary for Left-Right Asymmetry in the Zebrafish Embryo
… organ known as the Kupffer’s vesicle (KV) or the embryonic node, in zebrafish and mouse, respectively. Cilia beating within the KV are essential to generate a counterclockwise flow of fluid, which leads to left-sided Ca2+ elevation. Ca2+ is a crucial morphogenic second messenger during vertebrate …
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Development and Evaluation of 3D-Printed Cardiovascular Stents
… using in vitro cell culture studies and zebrafish embryo model. The percentage changes in volume of stents fabricated using HEMA and HEMA-0.35MeGO were 36.7±4.7% and 7.7±1.6%, respectively after 24 hr. The remaining fractions of 3D stents after 21 days and their corresponding first-order …
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The fathead minnow embryo as a model for the development of alternative testing methods in ecotoxicology.
… and a timeline for normal development of the embryo was established. Results showed only minor differences to already existing studies (Devlin et al. 1996, Manner and Dewese 1974). Based on fathead minnow-specific developmental data, the embryo toxicity test was adapted and refined. Using the …
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Notochord morphogenesis as a mechanical driver of embryo axis elongation
Early on in development, embryos undergo a dramatic change in shape in order to transform from a collection of cells into a functioning organism with a specialised body morphology. A key step in this process is the elongation of the head-to-tail, or anterior-posterior (AP) embryonic axis. For axis …
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Simultaneous visual and electro-cardiogram measurements of zebrafish embryos
… visual and electrocardiogram measurement of zebrafish embryos. One zebrafish embryo was placed between two electrodes and the electrical signal was amplified 100 times, then a computer recorded the data. The visual reading of the zebrafish heart rate was obtained by viewing the embryo under a …
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Function of core promoters in differential gene regulation during embryogenesis
… role in differential gene regulation using the zebrafish embryo as an in vivo model system. To analyse the functional requirement for the general transcription factor TATA-box binding protein (TBP), a diverse set of promoters was tested for their TBP dependence. This analysis revealed a …
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Cross-species single-cell transcriptomics and its applications in evolutionary and translational research
… which offers a new window into human embryogenesis and provides a resource for validating therapeutic targets, or observations from mice, in a non-rodent system. I then present three new approaches to visualise and compare single-cell transcriptomics data from multiple species. These …
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Cell Migration and Morphogenesis during Formation and Repair of Zebrafish Skeletal Muscle
I used the zebrafish embryo to investigate the morphogenesis of the developing somite and the behaviour of its different compartments in vivo via 3D confocal time-lapse microscopy. I demonstrated that the epaxial and hypaxial somite regions show distinct morphogenesis that lead to the chevron-shape …
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The role of cell division during tissue morphogenesis
<p>During embryonic tissue morphogenesis, cell division increases both the number of cells and cellular diversity. This is often regulated by the positioning of daughter cells post-mitosis. The goal of this dissertation research is to determine the mechanisms that place daughter cells, and how this …
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The use of metabolomics tools to assess the therapeutic natural products of honey and propolis from Malaysia and New Zealand
… and ovary), as well as phenotypic alteration on zebrafish embryo that can be related with activation of Notch signalling for anticancer study.
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Bioimaging of animal embryogenesis: mathematical methods and computational algorithms
Some fundamental biological processes such as embryonic development have been preserved during evolution and are common to species belonging to different phylogenetic positions, but are nowadays largely unknown. The understanding of cell morphodynamics leading to the formation of organized spatial …
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Chemical Genetic Studies of Tumor Suppressors in the Developing Zebrafish
The zebrafish is a powerful disease model system. Its distinct advantages allow it to be used for high-throughput genetic and chemical screens with high translational potential. One mutant isolated from a screen for cell cycle deficiencies is the zebrafish cdc25a mutant standstill. This mutant …
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Novel Oncogenic Drivers in Pediatric Gliomagenesis
… of downstream targets in primary astrocytes and zebrafish embryo ventralization. Whole-genome sequencing and RNASeq revealed that nearly half of our cohort contained structural variants. We identified recurrent gene fusions preserving the kinase domain of the neurotrophin family of receptor …
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Developmental Malformations in Zebrafish Caused by Exposure to Environmental Pollutants
… risk factors for causing birth defects. We used zebrafish as a model to identify and predict environmental chemicals causing congenital malformations. High-throughput screening of 292 unique chemicals from the ToxCast Phase I library identified 38 skeletal disruptor compounds (SDCs) producing …
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EPIDEMIOLOGICAL AND FUNCTIONAL APPROACHES TO EVALUATE THE EFFECTS OF PARTICULATE MATTER EXPOSURE IN INFANTS WITH VIRAL BRONCHIOLITIS
… following an acute infectious stimulus in a zebrafish (Danio rerio) embryo model. In particular, PM induced a pro-inflammatory effect, which leads to a dampened leukocytic response to a subsequent inflammatory stimulus. In the second part of the thesis, we investigated the relationship …
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Nodal organized cell dynamics for building tissue shapes in vertebrate gastrulation
… at the 128-cell stage animal pole of the zebrafish embryo, where Nodal is not normally expressed. The presence of Nodal+ cells led to a thickening of the animal pole blastoderm during the blastula stage. At the onset of gastrulation, the central region of the thickening internalizes, …
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Dynamics and Mechanics of Zebrafish Embryonic Tissues
… processes? Cell movements and tissue flow during embryogenesis constitute a beautiful problem of bridging scales: On the microscopic scale, cells are expressing particular genes which determine their identities and also their fate during morphogenesis. These molecular determinants then lead to the …
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Time-related alterations and other confounding factors in direct sediment contact tests
… (FA) and pentachlorophenol (PCP) in the zebrafish (Danio rerio) embryo SCT, the nematode SCT with Caenorhabditis elegans, and the bacterial SCT with Arthrobacter globiformis. The second experimental study assessed the impact of short-term (two week) contact-time between sediments and …
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