Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"yellow fluorescent protein"”.
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Protein localization during the cyanobacterial circadian cycle
… depends only upon the interactions between three proteins - KaiA, KaiB, and KaiC. As such, the cyanobacterial circadian clock provides a unique opportunity to study complex dynamics that can result just from the interactions between three proteins and how the signal from this core oscillator is …
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Methodology development for imaging histone modifications and for site-specific protein labeling in vitro and on the surface of living cells
… cells was developed using genetically encoded fluorescent reporters. These reporters were constructed for sensing histone phosphorylation and methylation by fluorescence resonance energy transfer (FRET). These reporters are four-part chimeric proteins with the domains from N- to C-terminus as …
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Quantitative Untersuchung der Verteilung, Mobilität und Bindung von fluoreszenzmarkierten Histonen in vitro und in vivo mit Fluoreszenzfluktuationsmikroskopie
… von Fluoreszenz untersucht. Dazu werden Histonproteine sowohl synthetisch mit organischen Farbstoffen konjugiert als auch die Fusionsproteine von H2B und H1 mit EYFP (enhanced yellow fluorescent protein) in HeLa-Zellen exprimiert. Die Messungen erfolgen mit Fluoreszenzkorrelationsspektroskopie …
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Characterization of a minimal abiotic stress-inducible promoter in the development of a binary vector for crop protection
… strong reporter transgene expression (enhanced yellow fluorescent protein, eYFP) under multiple abiotic stress conditions in transgenic tobacco (Nicotiana tabacum). The ABR1/ERF111 gene is a member of the group X AP2/ERF transcription factor (TF) family, which is transcriptionally responsive to …
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Biochemical Characterization of Two Aminopeptidases Involved in Hemoglobin Catabolism in the Food Vacuole of Plasmodium falciparum
… environment is unique for aminopeptidase P proteins and rare for M1 homologs. Our immunolocalization results have confirmed the dual distribution of these two enzymes in the parasite. Vacuolar targeting was found to be associated with the Plasmodium specific N-terminal extension found in the …
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Rapid Rac GTP-ASE activation in live cells by mechanical stress is independent of Src
… guanosine triphosphatase, was quantified using a fluorescent resonance energy transfer (FRET) method that measures changes in Rac activity in response to mechanical stresses by quantifying intensity ratios of ECFP (enhanced cyan fluorescent protein as a donor) and YPet (a variant yellow …
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The Impact of Chronic Morphine on Adult Hippocampal Progenitor Cells and the Neurogenic Niche
… the hippocampal neurogenic niche. I found that protein levels of factors within the niche were maintained or upregulated (e.g. vascular endothelial growth factor) to compensate for the morphine-induced decrease in proliferation. Lastly, I asked whether chronic morphine would decrease …
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The Role of the Scaffolding Protein INAD in Localization of Signaling Complexes to the Rhabdomeres of Drosophil Photoreceptors
Organization of proteins into macromolecular complexes is one way cells maximize the speed, specificity, and efficiency of signal transduction. In fruit fly photoreceptors InaD, a scaffolding protein containing 5 PDZ domains, organizes proteins involved in the visual signaling pathway into …
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Investigating Subcellular Thiol Redox Chemistry With Gfp-Based Redox Sensors
… are all dependent on cysteine-containing proteins, thus maintaining thiol-disulfide balance in this organelle is critical for cellular function. Thiol-disulfide equilibrium is primarily controlled by the reduced and oxidized forms of the abundant tripeptide glutathione (GSH and GSSG), …
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Molecular visualization and localization of ribosomal subunits interaction in cells
… and, more directly, by reports that ribosomal proteins are found associated with nascent transcripts on Drosophila polytene chromosome and that amino acids are incorporated at transcription sites. My project aimed to investigate further the question of whether there are functional ribosomes in …
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Correlating antisense RNA performance with thermodynamic calculations
… mRNA hybridization were computed based on the fluorescent protein reporter genes. Three different fluorescent proteins (i) green fluorescent protein (GFP), (ii) cyan fluorescent protein (CFP) and (iii) yellow fluorescent protein (YFP) were used as reporters. Each fluorescent protein was cloned …
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Subcellular and functional analyses of two small heat shock proteins and protein kinases from peroxisomes of Arabidopsis thaliana L.
… das Arabidopsis Proteom nach regulatorischen Proteinen durchsucht, welche eine mögliche Peroxisomen-Zielsteuerungssequenz 1 oder 2 (PTS) enthalten. Auf diese Weise konnten zwei kleine Hitzeschockproteine (sHsps - small heat shock proteins) und sieben Proteinkinasen identifiziert werden. Die …
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Vulnerability of ex vivo α-motor nerve terminals to hypoxia-reperfusion injury.
… finding that indicate that the oxygen storing protein, neuroglobin, maybe expressed at the mouse NMJ and report the difficulties of using mice that express yellow fluorescent protein (YFP) in their neurons for repeat/live imaging studies. Overall, these data show that the model of …
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Novel Fluorescent Sensors for Dynamic Intracellular Signalling
Protein kinase C (PKC), comprised of ten isozymes, is an abundant regulator of cellular function. PKC activity in living cells is commonly assayed in in vitro experiments with cell lysate. Monitoring PKC activity in single living cells is not possible with such experiments. This thesis presents a …
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Examining the Role of Phospholamban Phosphorylation on Interaction with SERCA Using Fluorescence Microscopy
… many forms of heart failure (HF), making these proteins potential targets for the treatment of HF. Both proteins have been extensively studied in vitro, where their basic structure and function were determined, and in animal models, where their role in disease was examined. However, key …
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Modelling Nuclear Body Dynamics in Living Cells by 4-D Microscopy, Image Analysis and Simulation
… density on diffusive mobility of the nuclear yellow fluorescent protein (YFP) appears marginal. A 2-D diffusion simulation to better characterize the experiment provides a tool to produce `diffusion maps' of the nucleus. The related aspect of nuclear body integrity and dynamics was examined …
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Ion channels and electrical excitability in native murine anterior pituitary corticotrophs
… promoter to drive the expression of enhanced yellow fluorescent protein (eYFP), to allow highly efficient and specific labeling and identification of corticotrophs in vitro. This approach, with patch clamp electrophysiological investigations, revealed metabolically intact native murine …
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Cell-type specificity and herbivore-induced responses of primary and terpene secondary metabolism in Arabidopsis roots
… to a reduced activity of the expressed TPS08-yellow fluorescent protein (YFP) fusion protein. When expression of TPS13 (producing the sesquiterpene (Z)-"-bisabolene) was directed from the cortex to the epidermis or stele, TPS13 gene expression and (Z)-"-bisabolene formation was supported by …
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Characterisation of PP71 homologues encoded by mammalian cytomegaloviruses
… in a regulated temporal cascade. The HCMV protein pp71, encoded by gene UL82, is located in the tegument of the HCMV virion and is delivered to cells immediately upon infection. This protein has been identified as a transactivator of viral immediate early gene expression. It also stimulates …