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Showing 1 to 5 of 5 for “"yeast three hybrid"”.
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The yeast three-hybrid system : a method for detecting ligand-protein interactions
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1996.
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Harnessing Saccharomyces cerevisiae Genetics for Cell Engineering
… evolution for in vivo, multi-component systems. Yeast hybrid assays provide versatile systems for coupling a function of interest to a high-throughput growth selection for directed evolution. In Chapter 2, we develop an experimental framework to characterize and optimize the performance of yeast …
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Charakterisierung der gamma-Untereinheiten AGG1 und AGG2 der heterotrimeren G-Proteine aus Arabidopsis thaliana
… der endogenen Promotoren exprimieren. Mit einem yeast three hybrid-Screen wurde AtVAP28 als Interaktionspartner von AGG2 identifiziert. AtVAP28 ist ein t-SNARE-ähnliches Protein und knüpft eine Verbindung zwischen heterotrimeren G-Proteinen und Vesikeltransport in Wurzeln. Die Existenz zweier …
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Development of chemical inducers of dimerization for screening competitive histone deactelyase inhibitors
<p>Histone Deacetylase (HDAC) proteins are transcriptional regulators that affect histone proteins, which are involved in packaging of DNA into chromosomes. HDACs have been linked to the proliferation of cancer through their role in transcriptional regulation. Due to these findings, HDAC inhibitors …
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The mechanism of HIV-1 Nef-mediated downregulation of CD4
… was subsequently confirmed in a human cell line. Yeast three-hybrid and GST pull-down assays were then used to demonstrate a robust, direct interaction between Nef and AP-2. This interaction was found to depend on a [D/E]xxxL[L/I]-type dileucine motif, located in the C-terminal loop of Nef, that …