Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 70 for “"yeast strains"”.
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Engineering industrial yeast strains for lignocellulosic ethanol production
This Dissertation was approved for publication on 2016-12-01 at 15:08.
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Isolation of low ethanol producing yeast strains using adaptive evolution
… reduced levels of ethanol relative to the strains they were derived from. Sub-lethal concentrations of sulfite, which sequesters acetaldehyde, thereby limiting ethanol production, was used as a selection pressure. However, removal of acetaldehyde not only limits ethanol production, it also …
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Isolation of antimutator yeast strains: an exploration of DNA replication fidelity
… increase in spontaneous mutation rate. Haploid yeast strains with combined defects in Pol proofreading and MMR initially divide, but succumb to error-induced extinction and fail to form viable colonies. We exploited error-induced extinction to isolate suppressors of lethal mutation rates (eex …
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Yeast flocculation: understanding cell surface structure-function relationships in industrial yeast strains
… of such controlled forms of cellular adhesion in yeast that occurs preferentially in the liquid environments is a process of asexual aggregation of cells which is also referred to as flocculation. The timing during growth and the causes of onset of yeast flocculation are of commercial interest to …
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Optimal yeast strains for the production of fuels and chemicals from lignocellulosic biomass
… for engineering efficient xylose-fermenting strains has not been finalized yet. In this thesis study, by elucidating fundamental bottlenecks impeding the xylose metabolism, I identified and implemented powerful genetic perturbation targets for developing an efficient xylose-fermenting …
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Engineering yeast strains for producing fuels and value-added chemicals from cellulosic biomass
… and chemicals. Most xylose-fermenting engineered yeast cannot utilize xylose until glucose is completely consumed. In addition, acetic acid derived from hemicellulose during the pretreatment negatively influences the fermentation performance by yeast. The overall goal of my thesis study is to …
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Metabolic engineering of yeast strains for renewable biomass utilization and valuable chemical production
… and biotechnology tools for developing optimal yeast strains capable of utilizing various sugars derived from renewable biomass and produce valuable chemicals. Sugars derived from lignocellulosic biomass, mainly cellobiose and xylose, cannot be assimilated by the industrial microorganisms, such …
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Engineering robust yeast strains for the conversion of xylose derived from lignocellulosic biomass to xylitol
… conferred to three natural isolate host strains via conventional and CRISPR-Cas9-mediated δintegration of three genes encoding a β-xylosidase, β-xylanase and xylose reductase (XR), was evaluated. The effect of over-expressed heterologous protein production on strain robustness and …
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Engineering and evaluation of yeast strains for the production of lactic acid from cellulosic sugars
… xylose-fermenting Saccharomyces cerevisiae yeast strain. Simultaneous co-fermentation of xylose and cellobiose for the production of lactic acid by yeast was achieved by the introduction of the ldhA cassette into an engineered, xylose- and cellobiose-fermenting S. cerevisiae. Through …
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Systematic and combinatorial approaches for metabolic engineering of optimal yeast strains to produce fuels and chemicals
… engineering approach for developing optimal yeast cell factories capable of efficiently fermenting various sugars abundant in renewable biomass. The research has broad applications for sustainable biological production of value-added fuels and chemicals. Initially, a systematic approach based …
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Metabolic engineering of industrial yeast strains to minimize the production of ethyl carbamate in grape and Sake wine
… carcinogen ethyl carbamate (EC). Urea degrading yeast strains were created by integrating a linear cassette containing the DUR1,2 gene under the control of the S. cerevisiae PGK1 promoter and terminator signals into the URA3 locus of the Sake yeast strains K7 and K9. The ‘self-cloned’ strains …
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Exploiting yeast diversity in whisky fermentations for biocatalysis of desirable flavour compounds
Yeast is an important component in producing flavourful fermented products. The Scotch Whisky industry currently only uses four different yeast strains all belonging to <i>Saccharomyces cerevisiae</i>. In recent years, the brewing and wine industries have proven that a variety of yeasts can impart …
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The anti-fungal and anti-oxidant properties of polyphenols extracted from the resurrection plant, Myrothamnus flabellifolia
… polyphenols on growth of S. cerevisiae yeast strains was investigated. This study showed that M flabellifolia polyphenols inhibited growth of both the wild type and the Δhsp 12 yeast strains largely by binding protein in the growth medium. A decreased specific growth rate, reduced …
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GCD10 and GCD14 Proteins Facilitate Tomato Bushy Stunt Viral (TBSV) RNA Replication in Saccharomyces Cerevisiae
… in wild type, GCD10 knockdown and GCD14 knockout yeast strains. Reduction of GCD10 protein and deletion of GCD14 protein in the yeast strains significantly inhibited TBSV subviral defective interfering (DI) RNA replication, suggesting that both GCD10 and GCD14 proteins facilitate TBSV DI …
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Physiological and Biochemical Characterization of Yeasts Active in the Degradation of Cheese Whey to Glycerol, Ethanol and 2,3-Butylene Glycol
… has involved determining to what extent specific yeast strains typically employed in the food industry, utilize lactose and produce commercially useful by-products. Cheese whey is largely a waste product of the dairy industry which contains approximately 5% lactose, and its untreated disposal …
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Rapid and high-resolution analysis of winemaking yeasts using MALDI-TOF MS : A thesis submitted in partial fulfilment of the requirements for the Degree of Doctor of Philosophy at Lincoln University
… ethanol, CO2 and other aromatic compounds by yeasts. Saccharomyces cerevisiae is the main species used for wine production, whereas the contribution of non-Saccharomyces yeasts to the distinctiveness of wine was not acknowledged until the 1980s. The indigenous yeasts present in the vineyard …
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Aneuploidy causes proteotoxic stress in Saccharomyces cerevisiae
… genomes. Systematic analyses of aneuploid yeast and mouse cells suggested that aneuploidy causes chromosome-specific effects elicited by the amplification of specific genes and general aneuploidy-associated phenotypes Here I describe a phenotype that is shared by most if not all aneuploid …
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Modest Differences in Juice Yeast Assimilable Nitrogen Concentration and Composition Influence Wine Chemistry and Impart Limited Sensory Differences in Virginia-grown Chardonel
Nitrogen is requisite for yeast growth and metabolism during wine fermentation, and contributes to wine flavor development. Understanding how yeast assimilable nitrogen (YAN) impacts wine chemistry and sensory properties helps inform nitrogen management decisions in both the vineyard and winery. …
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