Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 14 of 14 for “"yeast one-hybrid"”.
-
Probing PAX6-DNA interactions using high-throughput yeast one-hybrid assays and deep mutational scanning
… of residues. Here I used a combination of yeast one-hybrid and deep mutational scanning in competitive growth assays to probe the functional consequences of almost all the possible single amino acid variants in the paired domain of PAX6. The results showcase the capabilities of the assay to …
-
Analysis of DNA-binding Proteins in Yeast Saccharomyces Cerevisiae
… this issue. The first is a modification of the yeast "one-hybrid" assay. The second is probing protein microarrays with DNA sequence elements. Using these methods, I identified two proteins, Sef1 and Yjl103c, that bind to the same DNA sequence element. Sef1 and Yjl103c are little-characterized …
-
The study of transcriptional regulation of necrotrophic effector genes ToxA and Tox3 in the wheat pathogen Parastagonospora nodorum
… on the ToxA promoter. DNase I footprinting and yeast one-hybrid assay also did not identify protein candidates interacting with the ToxA promoter. Interestingly, the ToxA promoter showed ability to induce strong expression in the tested media after replacing the ToxA gene with GFP. In contrast, …
-
The study of transcriptional regulation of necrotrophic effector genes ToxA and Tox3 in the wheat pathogen Parastagonospora nodorum
… on the ToxA promoter. DNase I footprinting and yeast one-hybrid assay also did not identify protein candidates interacting with the ToxA promoter. Interestingly, the ToxA promoter showed ability to induce strong expression in the tested media after replacing the ToxA gene with GFP. In contrast, …
-
Towards Identifying Cis and Trans Regulators of Expression of Xylem Cysteine Protease 1 (XCP1) in Arabidopsis
… commercial commodity. Among the major components of wood are the elongated, thick-walled water-conducting cells known as tracheary elements. Understanding tracheary element differentiation and maturation is of scientific and commercial importance as it may lead to broad understanding of …
-
Antigenic Variation and Telomere Structure in Trypanosoma brucei
… loci called expression sites (ESs) and only one is expressed at any time, suggesting a stringent regulatory mechanism. Here, it is demonstrated that trypanosomes engineered to express two VSGs simultaneously from the same ES can multiply in cultures and infect animals normally. Mechanisms …
-
Tail-anchored Triphosphate Tunnel Metalloenzymes in Arabidopsis thaliana: An Investigation into the Molecular Mechanisms underlying their Biological Functions
… of senescence cues (i.e. ABA or dark treatment). One of the sites, S437, shows a strong increase in phosphorylation after dark treatment and I have shown that phosphorylation at S437 is crucial for TTM1's function in senescence. Our extensive in vitro kinase assays identified two MAP kinases that …
-
THE FEMALE GAMETOPHYTE: DEVELOPMENT AND FUNCTION
… formation and differentiation. I employed a yeast one-hybrid approach to identify EC1.1 regulators; the EC1 genes are specifically expressed in the female gamete and they are required for gamete fusion, therefore they are good candidates for clarify how gamete differentiation occurs in …
-
Discovery of New Protein-DNA and Protein-Protein Interactions Associated With Wood Development in Populus trichocarpa
… energy sources that are more carbon neutral. One such alternative energy source is to use the biomass derived from forest trees to fulfill the need for a renewable alternative fuel. Through increased understanding and optimization of regulatory mechanisms that control wood development the …
-
Function and Regulation of Xylem Cysteine Protease 1 and Xylem Cysteine Protease 2 in Arabidopsis
… The 237-bp region served as bait in a yeast one-hybrid analysis. Screening of yeast colonies retrieved 109 putative positive interactions, which included a potential transcriptional regulator, indole acetic acid-induced protein 8 (IAA8). An auxin responsive element that potentially …
-
Characterizing the transcriptional regulation of crassulacean acid metabolism in Kalanchoe
… Ppc1 promoter were used at bait probes in yeast one-hybrid (Y1H) assays. From this analysis, several high-confidence interacting transcriptional regulators were identified including ERF9, ERF106, TCP4, and PIF1. In silico examination of the Ppc1 promoter revealed likely binding sites for …
-
The functional characterisation of the XhABFA transcription factor from the resurrection plant Xerophyta humilis
… in resurrection plants, though downstream components of this network (such as the ABI3 regulon) are active. Recently, in a transcriptomic study of the resurrection plant Xerophyta humilis, it was found that the LAFL TFs are induced during seed maturation but not during vegetative desiccation. …
-
Protein factors and 5' flanking sequences involved in the expression of the mouse myelin basic protein gene.
… III - Sac I fragment of pEX1 plasmid was subcloned in the vector pBluescript. The cloned insert, which corresponds to the region between nucleotides -1319 and +227 relative to the transcription start site of the mouse MBP gene, was subsequently sequenced manually using the chain termination …
-
Nitrilase 2: insight into its regulation in Arabidopsis and its potential for improving maize salt tolerance
… of AtMYB2 to the AtNit2 promoter observed in a yeast one-hybrid (Y1H) assay, suggesting that it may not be involved in AtNit2 regulation. Although AtMYB30 did not bind directly to the AtNit2 promoter in the Y1H assay, AtMYB30 overexpressing plants were more salt tolerant and showed increased …