Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"yeast model"”.
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Characterizing CMT-causing variants in tryptophanyl- tRNA synthetase
… in these enzymes. I created a humanized yeast model to assess the underlying disease-causing mechanism associated with two CMT variants, H257R and D314G, present in the human tryptophanyl-tRNA synthetase (WARS1). Using proteomic analysis, protein biochemistry, and yeast growth assays, I …
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Development of the new yeast-based assays for prion properties
… monitor the conformational status of PrP in the yeast cells. In this system, the prion domain of Sup35 (Sup35N) was fused to PrP90-230, and the initial formation of the PrPSc-like conformation stimulated prion formation of Sup35N, which in turn converted soluble Sup35 into the prion isoform, …
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Evaluating the role of Exo1p in chromosome breaks at FS2 in Saccharomyces cerevisiae
… by homologous recombination at DNA breaks. A yeast model system was manipulated to induce replication stress, resulting in a break in DNA at fragile site FS2 on yeast chromosome III. The repair mechanism used was identified, and the resulting genome was analyzed. We did not observe increased …
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Using yeast to study neurodegenerative diseases : amyloid formation as a protective mechanism and a new Alzheimer's disease model
… cells ranging from E. coli to human stem cells. Yeast prion proteins are another group of proteins capable of adapting an amyloid conformation. The self-templating amyloid fold allows yeast prions to act as non-Mendelian elements of inheritance. We have shown that yeast prion amyloid fibrils, …
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Computational Software for Building Biochemical Reaction Network Models with Differential Equations
… physiology requires accurate mathematical models that depict the temporal dynamics of these chemical processes. Modelers build mathematical models of chemical processes primarily from systems of differential equations. Although developing new biological ideas is more of an art than a …
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The Chlamydia Trachomatis Protein Interaction Network: Insights into the Unique Composition of the Type Three Secretion System
… In the absence of genetic tools, we developed a yeast model system to identify and characterize chlamydial proteins involved in virulence mechanisms. In this study we describe the identification of twenty-eight proteins potentially involved in modulating host cellular functions and the secretion …
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Screening the effects of indoles on the proliferation of yeast via changes in lipid carbon flux
… cerevisiae and Schizosaccharomyces pombe yeast models. Lipids are essential to provide key elements to sustain cell growth and proliferation. In this study, indole, 2-PI and compound 209 were screened in an oleaginous yeast model, Lipomyces starkeyi (L. starkeyi), to ascertain their effect …
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Evaluation of the effect of growth conditions on lipid production by Papiliotrema laurentii UFV-1 in culture media containing xylose
… oil sources such as microbial oil. Oleaginous yeasts accumulate at least 20% of their dry weight as lipids, mainly in the form of triacylglycerols, nevertheless, superior values can be achieved depending on the culture conditions. Papiliotrema laurentii UFV-1, contrary to the oleaginous yeast …
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Protein misfolding toxicity and inclusion formation in cellular models of neurodegeneration
… study utilizes powerful genetic approaches in yeast and verification in mammalian neuronal cell lines to address the misfolding and toxicity of three proteins, the Rho Guanine Nucleotide Exchange Factor (RGNEF), Matrin3, which are involved in amyotrophic lateral sclerosis (ALS) and …
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Effects of nitrogenous substances on heat transfer fouling using model thin stillage fluids
… substances and protease on fouling using model and commercial thin stillage fluids. Nitrogenous substances urea and yeasts, as model protein sources in thin stillage, were mixed with glucose. Thermocouples in an annular probe were used to monitor surface temperature; fouling resistance was …
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Nanobody-Based Interactomic Studies of Single Transcripts During mRNA Maturation
… isolations of mRNPs, starting in a budding yeast model system. In our approach, a single target transcript is tagged with MS2 hairpin sequences – these hairpins are bound specifically and with high affinity by the bacteriophage MS2 coat protein (MS2CP). In the first purification step, a …
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Characterization of ARV1-Mediated Sterol Transport in Yeast and Mammalian Systems
… and mammalian systems by two approaches. In yeast, we used gene deletion to access loss of Arv1p function. In mammalian cells we utilized antisense oligonucleotides (ASOs) to decrease 𝘈𝘙𝘝1 expression 𝘪𝘯 𝘷𝘪𝘵𝘳𝘰 and 𝘪𝘯 𝘷𝘪𝘷𝘰. In the yeast model, loss of Arv1p function results in sensitivity to …
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Functions of RNA exosome and tRNA splicing ligase in Mendelian and Infectious diseases
… unknown.</p> <p>Here, I developed a hybrid yeast/mammalian RNA exosome model of exosomopathies by systematically replacing the individual yeast subunits with their corresponding human or mouse orthologs. This allows us to unambiguously assess the damaging effects of the exact patient variant …