Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"yeast genome"”.
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Compaction of chromatin in Saccharomyces cerevisiae
… the link between the association of the yeast linker histone homologue, Hholp, and the compaction of the yeast genome during stationary phase. The relative gene content of condensed chromatin, fractionated and isolated by sucrose gradient ultracentrifugation from stationary and …
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Analysis of transcriptional regulatory circuitry
… of transcriptional regulatory circuitry in yeast. These microarray technologies, expression analysis and location analysis (ChIP on CHIP), bring unique challenges for data analysis. I have adapted methods described previously and have developing new algorithms for addressing some of these …
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Communication between layers in biological transcriptional networks
… biologists to map where proteins bind in the yeast genome. The combinatorial binding of different proteins at or near a gene controls the transcription (copying) of a gene and the production of the functional RNA or protein that the gene encodes. Therefore, ChIP-chip data provides powerful …
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Genome-wide analysis of transcription factors and chromatin in S. cerevisiae
… encoding these factors have been identified in yeast, the role they play in regulating the expression of the yeast genome was not known. Genome-wide expression analysis of mutations in the Rpbl subunit of RNA polymerase II, the Srb4 subunit of Srb/Mediator complex, and the Kin28 subunit of TFIIH …
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Chromosome dynamics of the early meiotic cell cycle in S. cerevisiae
… DNA double-strand breaks (DSBs) into the genome to produce physical DNA exchanges, or crossovers, between homologous chromosomes. To investigate the chromosome dynamics of the early meiotic cell cycle, I performed comprehensive analysis of pre-meiotic DNA replication and DSB formation in …
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Microarray data analysis methods and their applications to gene expression data analysis for Saccharomyces cerevisiae under oxidative stress
… in controlled conditions using Affymetrix Yeast Genome S98 arrays. The oxidative stress response was measured at 8 time points along 120 minutes after the addition of CHP, with the earliest time point at 3 minute after the exposure. Statistical analysis methods, including ANOVA, k-means …
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Inhibition of Mitochondrial Translation as a Therapeutic Strategy for Acute Myeloid Leukemia
… hematopoietic cells (34% reduction, n=5). A yeast genome-wide screen identified mitochondrial translation inhibition as the mechanism of tigecycline-mediated cell death in eukaryotic cells. TIG decreased the expression of mitochondrial peptides, enzyme activity and membrane potential …
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In Vivo Studies of Yeast Mitochondrial Intron Splicing : Ectopic Branching and a Screen for Nuclear Encoded Splicing Factors
… two studies of group II intron splicing in yeast mitochondria. Previous studies done in collaboration with Dr. Anna Pyle at Yale identified several important determinants for in vitro branch-site selection of intron aI5gamma : the presence of a bulged A(A880), the 5' flanking GU base pair …
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STRUCTURAL AND FUNCTIONAL DELINEATION OF SUBUNITS AND DOMAINS IN THE SACCHAROMYCES CEREVISIAE SWI/SNF COMPLEX
… the prototype remodeler SWI/SNF from budding yeast. ySWI/SNF is a 12 subunit complex that includes the catalytic subunit Swi2/Snf2. It affects 6% of the yeast genome being primarily involved in gene activation. We employed a systematic protein or domain deletion strategy and characterized the …
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A Proteomic and Genomic Investigation Into Replication Fork Dynamics in Saccharomyces Cerevisiae
… associate with progressing replication forks in yeast. A focused proteomics approach is employed to specifically identify interactions between the replication fork-coupled GINS complex and other components of the replication machinery. The scope of this technique is extended by applying it to …
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Engineering a synthetic p53-Mdm2 network in budding yeast
… synthetic p53 network has been built in budding yeast with the aim of studying the network in isolation while being embedded in living cells. p53 and most proteins in the network are absent from the budding yeast genome. This diminishes the likelihood of interferences on the engineered module …
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Metabolic engineering of xylose-fermenting saccharomyces cerevisiae for bioisoprene production
… multi-copy isoprene synthase gene into CT2-QM genome. Every plasmid was digested with a specific restriction enzyme to provide homologous arms to yeast genome, resulting in integration of isoprene synthase into genome via homologous recombination. In doing so, multi-copy isoprene synthase gene …
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Mechanism of Incorporation and Repair of Uracil At Highly Transcribed Genes In Saccharomyces Cerevisiae
… The correlation between transcription and genome instability is largely explained by the topological and structural changes in DNA and the associated physical obstacles generated by the transcription machinery. However, such explanation does not directly account for the unique types of …
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A Pharmacological Strategy Against African Sleeping Sickness
… is essential in <em>T. brucei </em>(tbdUTPase), yeast (yDut1), and humans (hDUT). dUTPase being a dimer in <em>T. brucei</em> but a trimer in yeast and humans, we hypothesize there may be a molecule that inhibits the function of<em> </em>tbdUTPase but not of yDut1 and hDUT. The endogenous yDut1 …
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Probing PAX6-DNA interactions using high-throughput yeast one-hybrid assays and deep mutational scanning
… of residues. Here I used a combination of yeast one-hybrid and deep mutational scanning in competitive growth assays to probe the functional consequences of almost all the possible single amino acid variants in the paired domain of PAX6. The results showcase the capabilities of the assay to …
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Deciphering genome-wide chromatin occupancy, dynamics, and their connections to gene regulation
… of the cell. The proteins can bind to the genome in different combinations leading to a combinatorial explosion in the number of possible chromatin configurations. The differences in the chromatin configurations for the same genome sequence give rise to distinct cell types. Likewise, cells …
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Determining the Roles of the Aldehyde Dehydrogenase Enzymes 5 and 6 during Icewine and Table Wine Fermentation in a Commercial Wine Yeast Using CRISPR-Cas9.
… (40°Brix) causes hyperosmotic stress for yeast, resulting in water loss, triggering glycerol production to act as an internal osmolyte to draw water back into the yeast cell, resulting in an NAD+ imbalance. Icewine has significantly higher acetic acid versus table wine, where acetic acid …
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Effect of low temperature on Sauvignon blanc fermentation by Saccharomyces cerevisiae
… during fermentation, comprising 40 % of the yeast genome. Five key metabolic pathways are strongly influenced by cold fermentation: nitrogen, iron/copper, sulfur, vitamins (biotin and thiamine) and oxidative stress. Yeast strains differ in their transcriptional responses to cold fermentation …
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An RNA-interference-based platform for genome analysis and engineering in Saccharomyces cerevisiae
… mutations globally and iteratively on a genome scale. Thanks to its simplicity and effectiveness, the pooled RNA interference (RNAi) screening should satisfy the requirement of directed genome evolution. The RNAi pathway is absent in Saccharomyces cerevisiae, also known as the baker’s …
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Localisation and function of phosphoinositide-specific phospholipase C in Sacchromyces cerevisiae
… PLC of the \(\delta\) family in the budding yeast Sacchromyces cerevisiae. It plays a key role in generating Ins(1,4,5)P\(_3\) in this fungi in response to stress and yet the yeast genome encodes no Ins(1,4,5)P\(_3\) receptor. This suggests that the functions of PLC-\(\delta\)s are mediated …
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