Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"yeast genetics"”.
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Structural and Functional Evolution of Human Heat Shock Transcription Factors
… utilize a diverse array of techniques including yeast genetics, recombinant protein expression and purification, biochemical analysis of protein-DNA interactions, x-ray crystallography, in vitro post-translational modification, and mammalian cell culture to illuminate novel aspects of HSF …
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On Sulfur Sensing in Saccharomyces cerevisiae
… levels of sulfur-containing metabolites. In the yeast Saccharomyces cerevisiae, regulation of sulfur metabolism can be distilled down to the actions of two proteins; the F-box protein Met30, and the transcriptional coactivator Met4. Met30 belongs to the family of SCF (Skp1-Cul1-F-box protein) E3 …
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Roles of Phospholipase A/Acyltransferase Enzymes in Membrane Remodelling and Organelle Degradation
… A through a multi-system approach combining yeast genetics, mammalian cell models, and lipidomics. In wild-type (wt) yeast, human PLAAT4 activity increased the size of lipid droplets, suggestive of fatty acid accumulation. The activity of PLAAT4 also resulted in ER morphological defects and …
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The Assembly of Pathogenic Signaling Circuits by a Family of Bacterial Secreted Effector Proteins
… utilize to assemble circuits, we utilize yeast genetics to identify novel membrane-interactions. We identify for the first time the direct association of the Shigella GEF IpgB1 with acidic phospholipids. Surprisingly, we find that these protein-lipid interactions are not required for …
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Structure and activation of the class D GPCR dimer Ste2
… a prototypical class D GPCR found in the baker’s yeast Saccharomyces cerevisiae and is critical for pheromone-sensing and sexual mating in yeast. Ste2 was the first ligand-binding GPCR to be sequenced, and advances in yeast genetics have allowed an extensive characterization of Ste2 and its …
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Harnessing Saccharomyces cerevisiae Genetics for Cell Engineering
… evolution for in vivo, multi-component systems. Yeast hybrid assays provide versatile systems for coupling a function of interest to a high-throughput growth selection for directed evolution. In Chapter 2, we develop an experimental framework to characterize and optimize the performance of yeast …
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Building a Genetic System in Yeast to Search for High Affinity Proteins in Sequence Space
… 2, I discuss the use of phenotypic selection for yeast-display protein binders, and test these systems on simple loop libraries. In Chapter 3, I construct a genetic system in yeast that can mutate a protein loop via homologous recombination, and test its recombination function. In Chapter 4, I …
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Characterization of ARV1-Mediated Sterol Transport in Yeast and Mammalian Systems
… and mammalian systems by two approaches. In yeast, we used gene deletion to access loss of Arv1p function. In mammalian cells we utilized antisense oligonucleotides (ASOs) to decrease 𝘈𝘙𝘝1 expression 𝘪𝘯 𝘷𝘪𝘵𝘳𝘰 and 𝘪𝘯 𝘷𝘪𝘷𝘰. In the yeast model, loss of Arv1p function results in sensitivity to …
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Structure and Function of the ER-Membrane Complex
… the EMC I set out to solve the structure of the yeast EMC. Structural characterisation via cryo-electron microscopy (cryo-EM) yielded a 6.7Å resolution map that revealed the overall architecture of the complex and multiple features that had not been predicted from previous studies. An appendix …
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PHDS, Stress, And Starvation: The Identification Of A New RPD3 Deacetylase Complex Involved In The Yeast Oxidative Stress And Metabolism Pathways
… some of the 17 PHD fingers of the budding yeast Saccharomyces cerevisiae, these questions remain unanswered. In the research presented in this thesis, I sought to gain insight into the ligands and functions for three yeast PHD finger proteins, the Yng1 subunit of the NuA3 acetyltransferase …
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REDEFINING THE SCOPE OF PRENYLATION: DISCOVERY OF “FORBIDDEN” SUBSTRATE RECOGNITION AND DEVELOPMENT OF METHODS UTILIZING PRENYLATED PROTEINS
… However, recent genetic screening studies in yeast suggest the potential for FTase to prenylate sequences of the form -C(x)3X, with four amino acids downstream of the cysteine residue to be prenylated. The work herein begins to define the sequence scope for this -C(x)3X motif, establishes the …
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Linking Architecture and Function of Endoplasmic Reticulum - Plasma Membrane Contact Sites
… (cER) - plasma membrane (PM) contacts in budding yeast cells is mediated by a set of six conserved proteins. By combining correlative light and electron microscopy (CLEM) and electron cryo-tomography (cryo-ET) with yeast genetic techniques, this work addresses unanswered questions about the …
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Pyp3 Involvement in Mitotic Control and Cell Growth in Fission Yeast
In fission yeast, Schizosaccharomyces pombe, tyrosine dephosphorylation of p34cdc2 is required for mitotic initiation and this reversible tyrosine phosphorylation plays a crucial role in the regulation of the cell cycle. The kinases wee1+, mik1+ and the phosphatases cdc25+, pyp3+ play opposite …