Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"xylose reductase"”.
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Optimizing xylitol production: a molecular and experimental study of xylose reductase in Candida tropicalis
… the ability of Candida tropicalis to convert D-xylose into xylitol through the enzyme xylose reductase, with a focus on optimizing pH conditions to enhance conversion efficiency. To achieve this, both experimental fermentation and computational molecular dynamics simulations using GROMACS and …
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Xylitol Production From D-Xylose by Facultative Anaerobic Bacteria
… 10 of the 17 species screened could grow on D-xylose and produce detectable quantities of xylitol during 24-96 h of fermentation. The ten bacterial species were studied for the effect of environmental factors, such as temperature, concentration of D-xylose, and aeration, on xylitol production. …
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Diversity of yeasts associated with wood and the gut of wood-feeding insects
… sugars. The consistent association between xylose-fermenting (X-F) yeasts and the gut of lignicolous insects has been used as evidence of a symbiotic relationship between them. In general passalid beetles (Passalidae) and wood-roaches (Cryptocercidae) feed on rotted wood where they spend …
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Engineering robust yeast strains for the conversion of xylose derived from lignocellulosic biomass to xylitol
… genes encoding a β-xylosidase, β-xylanase and xylose reductase (XR), was evaluated. The effect of over-expressed heterologous protein production on strain robustness and metabolism was also assayed. Our results revealed that the overexpressed XR failed to improve on the xylose reduction ability …
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Precision fermentation for enhanced production of food ingredients, fermented foods, and chemicals
… by changing expression levels of genes (XYL1, xylose reductase and GDH, galactitol dehydrogenase). The dairy industry generates lactose-rich permeates as a by-product, which can be fermented to produce value-added compounds like tagatose, a low-calorie sweetener. Engineered yeast strains …
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Optimal yeast strains for the production of fuels and chemicals from lignocellulosic biomass
… hexose and pentose sugars, mainly glucose and xylose, which are not efficiently fermented by microbes. Despite numerous efforts for improving xylose fermentation during the last two decades, a method for engineering efficient xylose-fermenting strains has not been finalized yet. In this thesis …
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Engineering a Fungal L-Arabinose Pathway Towards the Co-Utilization of Hemicellulosic Sugars for Production of Xylitol
… can utilize both hemicellulosic pentose sugars D-xylose and L-arabinose from renewable plant biomass as feed substrates, my thesis research focused on utilizing a fungal-derived biosynthetic pathway for production of xylitol. The pathway consists of two NADPH-dependent reductases, xylose reductase …
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Engineering a Fungal L-Arabinose Pathway Towards the Co-Utilization of Hemicellulosic Sugars for Production of Xylitol
… can utilize both hemicellulosic pentose sugars D-xylose and L-arabinose from renewable plant biomass as feed substrates, my thesis research focused on utilizing a fungal-derived biosynthetic pathway for production of xylitol. The pathway consists of two NADPH-dependent reductases, xylose reductase …
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Systematic and combinatorial approaches for metabolic engineering of optimal yeast strains to produce fuels and chemicals
… deletion of COX9 and serial subculture in xylose minimal media, was applied to improve ethanol fermentation by a xylose-fermenting yeast strain previously developed in our group (SR8). Genome sequencing and a yeast mating experiment led to identification of a frameshift mutation in a …
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Synergy of protein and genome engineering for fuels and chemicals production
… of hemicellulosic hydrolysate sugars. First, a xylose-specific xylose reductase enzyme was engineered for higher substrate specificity. However, after significant loss in enzymatic activity, genome engineering was targeted as an orthogonal strategy to increase substrate specificity further. The …
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Metabolic engineering of Saccharomyces cerevisiae for efficient ethanol production from pentose sugars
… Among them, one arabinose-specific and two xylose-specific transporters from Pichia stipitis and Neurospora crassa were identified. All transporters were functionally expressed and properly localized in S. cerevisiae as indicated by HPLC analysis and immunofluorescence microscopy, …
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Metabolic engineering of yeast for xylose uptake and fermentation
Xylose is the major pentose and the second most abundant sugar in lignocellulosic feedstocks. Therefore, the efficient utilization of xylose is required for the cost-effective bioconversion of lignocellulose. Rational and combinatorial metabolic engineering approaches coupled with transcriptomic …