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Showing 1 to 20 of 54 for “"xylanase"”.
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Cloning and expression of xylanase variants in Pichia pastoris
Submitted in fulfilment for the requirement of a Degree of Master in Biotechnology, Durban University of Technology, Durban, South Africa, 2017.
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Design characterisation and in planta expression of a chimeric xylanase
… was determined. Xyl22L did not retain catalytic xylanase activity but was able to accumulate in transgenic plant biomass, and expression of Xyl22L was strongly correlated with an increase in transgenic plant biomass. Fluorescent confocal microscopy showed that Xyl22L was associated with the …
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Development of a flat sheet woven fabric membrane fermenter for xylanase production by Thermomyces lanuginosus
Submitted in fulfilment of the requirements for the degree of Master of Engineering, Durban University of Technology, Durban, South Africa, 2015.
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Improvement of engineered thermostable xylanase production via immobilization of recombinant escherichia coli onto graphene oxide
… and ecologically available enzyme such as xylanase. In this study, the effects of graphene oxide (GO) on xylanase excretion and ~-galactosidase activity of immobilized E. coli was examined. The experiments were performed under the optimized RSM conditions (20°C, 0.5 mM, IPTG and pH 7) using …
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Molecular Analysis of a Cellulase/xylanase gene,celD, From the Ruminal Bacterium Ruminococcus Flavefaciens Fd-1
… cloned gene encodes both endoglucanase and endoxylanase activities. CMC and xylan zymograms of protein produced by FD1-1 and separated by non-denaturing PAGE suggest that the endoglucanase and endoxylanase activities reside on the same polypeptide. Transposon mutagenesis using Tn5supF localized …
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Digestibility of energy and total dietary fiber by gestating and lactating sows but not reproductive performance are influenced by exogenous xylanase
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2023-04-12 without embargo terms
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Determination of the Optimal Time in the Lactation Cycle to Start Feeding a Direct-Fed Cellulase/Xylanase Enzyme Mixture to Dairy Cows
… cows to a direct-fed mixture of cellulase and xylanase enzymes (1.25 L of enzyme concentrate/tonne of forage dry matter) applied to the forage portion (60% corn silage and 40% alfalfa hay) of a total mixed diet starting either in theclose-up dry period, at calving, or at peak milk production. …
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Two thermostable hemicellulases and their effects on wood pulps
… of radiata pine kraft pulp were examined. The xylanase (two preparations) and mannanase had broad pH optima (6-7.5). The xylanases had temperature optima of 70°C (10 min assay) and the mannanase had an optimum of 80°C (10 min assay). All the cloned enzymes were thermostable with more than 95% …
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Evaluation of the precision-fed rooster assay for detecting effects of exogenous carbohydrases and adaptation diet on true metabolizable energy and cecal microbial ecology, short-chain fatty acid profile, and enzyme activity
… six combinations of various α-galactosidase and xylanase levels on TMEn of a corn/soybean meal diet, while Experiment 2 evaluated two levels of a β-glucanase and xylanase on TMEn of pearled barley and rye diets, respectively. Two levels of a multi-carbohydrase combination were also evaluated for …
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Carbohydrate composition, in vitro digestion, and effects of xylanase and phytase on nutrient and energy digestibility by pigs in grains and grain coproducts
… composition, in vitro digestion, and effects of xylanase and phytase on ileal and total tract digestibility of nutrients and energy in grains and grain coproducts. The objective of Exp. 1 and Exp. 2 was to determine the efficacy of xylanase and phytase supplementation to growing swine diets based …
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Effects of exogenous enzymes alone or in combination with oligosaccharides on utilization of total dietary fiber, fermentation, growth performance, and health of weanling and growing pigs
… enzymes and the combination of the enzyme xylanase with oligosaccharides on the utilization of dietary fiber, fermentation, digestibility of energy and nutrients, growth performance, and health of weanling and growing pigs. Experiment 1 was conducted to establish a database for soluble …
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Analysis of genes and enzymes involved in the degradation of hemicellulose and cellulose by Butyrivibrio fibrisolvens H17c
… and to isolate genes expressing cellulase and xylanase activity. Two genes encoding β-1-4- glucosidase (BglA) and endo-β-1-4-xylanase (XynB) were cloned in E. coli.
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Investigation of host cell wall degrading strategies of Pseudomonas syringae pv. actinidiae (Psa)
… This research investigated whether Psa produces xylanases to degrade cell wall material of kiwifruit stems and facilitate systemic mobility of the pathogen from the infection site. The research also investigates whether vessel anatomy and xylem architecture play a role in the systemic mobility of …
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Cloned xylanolytic and cellulolytic enzymes from an extreme thermophile
… It showed some transferase activity. The xylanase had high activity on xylan, releasing xylobiose and some xylotriose. It also hydrolysed carboxymethylcellulose, 4-methylumbelliferyl β-D-cellobioside (MUC) and p-nitrophenyl β-D-cellobioside but could not hydrolyse xylobiose. It showed …
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Effects of Feeding Hulless Barley (Hordeum vulgare L.) and Supplementing a Fibrolytic Enzyme on Production Performance, Nutrient Digestibility, and Milk Fatty Acid Composition of Lactating Dairy Cows
… of feeding hulless barley and supplementing a xylanase enzyme on production performance and nutrient utilization of lactating dairy cows. In study 1, we evaluated production performance, milk fatty acid composition, and nutrient digestibility in high-producing dairy cows consuming diets …
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Effects of amylase supplementation upon the growth, endogenous amylase activity, and intestinal morphology of male turkey poults
… the effect of varying levels of amylase and xylanase on bird performance, and to determine optimal level of enzyme inclusion in the basal corn-soybean meal diet. Feed utilization or growth of birds did not differ between the amylase diets, however 200 units of amylase per kilogram of feed …
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Phytase Impacts Various Non-Starch Polysaccharidase Activities on Distillers Dried Grains with Solubles
… cellulase (0, 1.5, 15, and 150 U/g of DDGS), xylanase (0, 2.5, 25, and 250 U/g of DDGS), ï ¢ glucanase (0, 0.006, 0.06, and 0.6 U/g of DDGS), and phytase (0, 250, 500, and 1,000 FTU/kg of DDGS). Results indicated an inhibitory effect of phytase on the ability of NSPases to release reducing …
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Effect of Corn Quality and Enzyme Supplementation on Broiler Performance, Gastrointestinal Enzyme Activity, Nutrient Retention, Intestinal Mucin, and Jejunal Gene Expression
… cocktail enzyme of amylase, protease, and xylanase on broiler performance from 0 to 49 days of age. In each of the first two EXP, the four diets used consisted of (1) diet AR-/BR-; reduced dietary energy by increasing corn A matrix metabolizable energy (ME) 138 kcal/kg in EXP 1 or 125 …
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Nutritional evaluation of rice coproducts fed to pigs
… rice, and broken rice is improved if microbial xylanase is added to the diet was tested. Results indicated that concentrations of DE and ME (DM basis) in FFRB and DFRB increased (P < 0.05) if xylanase was used. Broken rice had a greater (P < 0.05) concentration of DE and ME than FFRB and DFRB if …
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A comparative study of certain soil-borne cereal pathogens with reference to carbohydrate utilisation and polysaccharide hydrolysis
… isolates produced pectin degrading enzymes, xylanase and carboxymethylcellulase when grown in a salts medium with an appropriate carbon source. There was little difference in the amounts of enzymes produced by the organisms except for the greatly enhanced endo-poly-rnethylgalacturonase …
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