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Showing 1 to 7 of 7 for “"xenopus egg extract"”.
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Protein Phosphatase 1 at the Kinetochore Regulates Chromosome Segregation
… Using the biochemical tools available in the Xenopus egg extract system, I show here that PP1 binds to the protein KNL1 (Spc105, Blinkin, CASC5) through an evolutionarily conserved RVxF motif. KNL1 is a member of the KMN network that forms the microtubule binding interface at the kinetochore. …
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The Influence of DNA Methylation on Both H2A.Z Nucleosome Dynamics and the Zinc Finger and Homeoboxes Transcription Factor Family
… and the use of crude physiological cytoplasmic extract from Xenopus laevis eggs coupled with sperm nuclei, we confirm that the H2A.Z and DNA methylation antagonism is conserved in Xenopus laevis. We further find that the degree of this H2A.Z and DNA methylation antagonism increases, and that the …
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The Role of the Protein MTBP in DNA Replication
… the role of MTBP in DNA replication using the Xenopus egg extract cell-free system. I found that MTBP and Treslin co-immunoprecipitate in metaphase and interphase extracts, suggesting that they form a complex throughout the cell cycle. MTBP, like Treslin, is recruited onto chromatin before the …
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Regulated Recruitment of the Chromosomal Passenger Complex to Chromatin and Microtubules Promotes Accurate Cell Division
… the INCENP SAH domain supports the SAC. Using Xenopus egg extract and human tissue culture cells, we show that the kinase domain of Haspin is autoinhibited during interphase by a previously unidentified Haspin basic inhibitory segment (HBIS) downstream of the kinase domain. Upon entry into …
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Linker Histone Medicated Regulation of Mitotic Chromosome Compaction and Individualization
… condensins and topo II to mitotic chromatin in Xenopus egg extracts. Using an in vitro reconstitution system, I showed that H1.8 inhibits binding of purified condensins and topo II to nucleosome arrays. I also showed that condensin binding to nucleosome arrays is sensitive to magnesium dependent …
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Molecular mechanisms of Sorting nexin-9 in membrane triggered actin polymerisation
… of SNX9 membrane binding domains in Xenopus egg extract-based actin polymerisation assays demonstrates that PX-BAR domains are es- sential for two distinct actin architectures. To visualise this, I used Cryogenic Electron Tomography (cryo-ET) to study the actin nucleating from 10% …
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Regulation of Anaphase Promoting Complex/Cyclosome to Control M Phase Exit
… </p> <p>Prior to fertilization, vertebrate eggs are arrested in metaphase of meiosis II by CSF activity, a key component of which is the APC/C inhibitor Emi2. Although the roles and regulation of Emi2 in maintaining CSF arrest have been extensively studied, its function during the oocyte …