Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"xenograft tumor"”.
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The Endoplasmic Reticulum Udpase ENTPD5 Promotes Cancer Cell Growth and Survival in the PI3K/PTEN
… is up-regulated in cell lines and primary human tumor samples with active AKT. AKT upregulates ENTPD5 by relieving transcriptional inhibition by FoxO transcription factors. ENTPD5 hydrolyzes UDP to UMP to promote protein N-glycosylation and folding in ER. Knockdown of ENTPD5 in PTEN-null cells …
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Development of Hif-1Α/Hif-1Β Heterodimerization Inhibitors Using A Novel Bioluminescence Reporter Assay System For In Vitro High Throughput Screening and In Vivo Imaging
<p>Tumor growth often outpaces its vascularization, leading to development of a hypoxic tumor microenvironment. In response, an intracellular hypoxia survival pathway is initiated by heterodimerization of hypoxia-inducible factor (HIF)-1α and HIF-1β, which subsequently upregulates the expression of …
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Development of Rational Combination Therapy With Parp Inhibitors and Kinase Inhibitors In Tnbc
… repair, cell cycle, cell proliferation and xenograft tumor growth. Similar to the c-Met-PARP1 axis, CK2 interacts with PARP1 in the nucleus. Moreover, CK2 can phosphorylate PARP1 <em>in vitro</em>, implicating that similar to c-Met, CK2 regulates PARP1 activity through direct …
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The role of NK cells in selectin-dependent tumor suppression
… that, in the absence of selectins, human tumors transplanted subcutaneously into Rag2'- mice grow significantly larger. This implicates selectins and the innate immune system in tumor immune surveillance. We have extended the xenograft tumor model to immunocompetent C57BL/6 mice. Similarly …
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Novel Effects of Simvastatin on Uterine Fibroids
… reaching effects in vivo which include anti-tumor effects. Statins inhibit the growth of multiple tumors; however the mechanisms remain incompletely understood. The purpose of this study was to examine the effects of simvastatin on uterine fibroid, both in vitro and using an animal model. For …
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Genetic Approach to Discover ARMC4 as a Novel NF-κB Negative Regulator and Tumor Suppressor in Colorectal Cancer
… and unsurprisingly, significantly decreased xenograft tumor growth in vivo. In contrast, shARMC4 knockdown cells showed quite opposite effect. Furthermore, co-immunoprecipitation (Co-IP) experiment confirmed that ARMC4 may form a complex with the p65 subunit of NF-κB. Importantly, …
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Novel Posttranslational Modification In Lkb1 Activation and Function
… has come to attention is that many oncogenes and tumor-suppressors are intimately linked to metabolic regulation (Jones and Thompson, 2009). One of the key tumor-suppressors involved in metabolism is Liver Kinase B1 (LKB1). LKB1 is the major upstream kinase of the evolutionarily conserved …
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The Protumorigenic Role of Caspase-8 in Neuroblastoma
… (NB), the most common extracranial solid tumor in children, accounts for 15% of cancer-related deaths in pediatric patients. Caspase-8 (casp8), a proapoptotic protein, is silenced in approximately, 50-70% of neuroblastoma patient samples. Loss of casp8 has been suggested to increase NB …
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Hypoxic gene regulation and oncogenic pathways in neuroblastoma
… ranging from spontaneous regression to fatal tumor progression despite intense multi-modal treatment. Previous studies have shown that hypoxia pushes neuroblastoma cells towards a more immature phenotype, which correlates to aggressive disease. Here we define a role for the hypoxia inducible …
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Krupple-Like Factor 4 Regulation in Intestinal Epithelial Differentiation, Proliferation and Polarity
… In colorectal cancer, KLF4 was identified as a tumor suppressor; it is also a stem cell factor that along with other factors can induce pluripotent stem cells. KLF4 knockout mice demonstrate decrease in goblet cell number in the colon; conditional KLF4 ablation from the intestinal epithelium …
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Delivery of molecular-specific optical contrast agents for cancer biomarker detection in live cells and tissues
… optical contrast agents was tested in xenograft tumor specimens co-treated with Triton-X100. These experiments revealed that Triton-X100 can facilitate simultaneous labeling of clinically relevant intracellular and extracellular biomarkers in a controlled, uniform manner. Together, …
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A Preclinical Model of Ionizing Radiation and TNF-alpha Gene Therapy for Cancer
<p>Long term control of high-grade brain tumors is rarely achieved with current therapeutic regimens. This study sought to determine if pretreatment with plasmid DNA expressing Tumor Necrosis Factor-α (TNF-α) could enhance the effect of ionizing radiation in an aggressive, rapidly growing glioma …
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Small Molecule Regulator of ENTPD5, and ER Enzyme in the PTEN/AKT Pathway
… synthesis, cell proliferation and survival. Tumor supressor gene PTEN encodes a lipid phosphatase that antagonizes PI3K function and consequently inhibits downstream signaling through Akt. Dysregulation of this pathway has been found in a variety of human cancer, mainly by loss of function of …
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Functional Characterization of Protein Tyrosine Phosphatases in Tumorigenesis through Substrate Identification
… dissect the role of pY128 p130Cas in colorectal tumorigenesis, we engineered p130Cas Y128F mutant knock-in colorectal cancer cell lines. The p130Cas Y128F mutant cells display reduced transformation ability <i>in vitro</i> and xenograft tumor growth <i>in vivo</i>. Mechanistically, the p130Cas …
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Prolyl Isomerase Pin1 Is a Conditional Tumor Suppressor
… Pin1 substrates involved in cell cycle and tumorigenesis. During cell cycle progression, the timely synthesis and degradation of key regulatory proteins is required to maintain genomic integrity. Previously, we determined that Pin1 binds to and promotes the degradation of the oncoprotein …
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Characterization and informed design of downregulating anti-epidermal growth factor receptor antibodies
… the extensive characterization of its role in tumor growth, epidermal growth factor receptor (EGFR) has long been an attractive target for monoclonal antibodies. Intense research has culminated in the approval of two antibody-based drugs against EGFR for cancer treatment, with numerous others …
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Identification of a new tumor suppressor pathway modulating rapamycin sensitivity in colorectal cancer
… the fundamental mechanisms for inactivation of tumor suppressor genes in many cancers. Thus, identification of these silencing tumor suppressor genes could provide insight into the biological processes and pathways underlying tumorigenesis. In this thesis, we provide a comprehensive approach …
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Determining the Oncological and Immunological Effects of Histotripsy for Tumor Ablation
… to generate acoustic cavitation within a tumor to mechanically fractionate targeted tissues. While pre-clinical work has demonstrated the feasibility of applying histotripsy to solid tumors including primary liver and renal tumors, there is still a need to investigate the potential of …
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Effect of uptake pathway on non-viral gene delivery in vitro and in vivo
… in HeLa and MDA-MB-231 cell cultures and xenograft tumors. First, we attached transferrin and folate ligands to PEI to direct internalization by clathrin- and caveolin-mediated mechanisms, respectively, and transfected HeLa and MDA-MB-231 cells with targeted and untargeted PEI in the …