Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 337 for “"whole-cell"”.
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Cytochrome P450 whole cell biohydroxylation of alkanes
… such as CYP153A6 have previously shown excellent 95% selectivity for hydroxylation at the terminal carbon on n-octane and CYP153A13 has shown promising activity on n-octane and n-decane. In the present study, whole cell biocatalysts were prepared by expressing CYPs in E. coli BL21(DE3) and …
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ACCELERATING WHOLE-CELL BIOCATALYSIS BY ENHANCING OUTER MEMBRANE PERMEABILITY
Whole-cell biocatalysts are preferred in many biocatalysis applications. However, cell envelope often represents a formidable permeability barrier. As a result, reactions catalyzed by whole-cells are reportedly orders of magnitude slower than those of by their free enzyme counterparts. The present …
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Advancing Fluorescence Microscopy Techniques for Volumetric Whole-cell Imaging
… to modern biology, revealing new insights into cellular structure and dynamics. However, imaging methods capable of three-dimensional (3D) imaging at high spatial and temporal resolution are still lacking. This thesis describes two projects aimed at advancing volumetric whole-cell imaging based …
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Development of rapid mass spectrometric methods for analyzing whole cell microorganisms
Includes bibliographical references (pages 138-148).
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Development and Characterization of an Autologous Whole Cell Breast Cancer Vaccine
… vaccine may be able to eliminate remaining tumor cells following primary treatment, prevent a recurrence and result in improved survival. </p> <p>We developed an autologous breast cancer vaccine using two murine cell lines, 4T1 and EMT6, to demonstrate the potential of vaccines for adjuvant …
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Mechanism of Action Studies of Phenotypic Whole-cell Active Antimalarial Leads Through Target Identification
… action for the treatment of malaria. Phenotypic whole-cell screening, followed by medicinal chemistry optimization efforts, identified the pyrido[1,2-a]benzimidazole compounds KP68 and KP124 and the benzimidazole compound DM253 as efficacious antimalarial leads. However, the essential details of …
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Validation and Application of a Novel Target-Based Whole-Cell Screen to Identify Antifungal Compounds
… we have devised a second-generation target-based whole-cell screening assay based on the principles of competitive fitness, which can rapidly identify target-specific and physiologically-active compounds. Briefly, strains expressing high, intermediate, and low levels of a preselected target …
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The identification and characterization of novel antibacterial compounds via target-based and whole cell screening approaches
Item withdrawn by Alexis Thompson (athmpsn1@illinois.edu) on 2011-01-19T21:59:21Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Wang_Nora.pdf: 12154315 bytes, checksum: a4abc355201eaebcd098c4d086069f75 (MD5)
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Application of whole-cell biosensor for the impact of crude oil exploration on the environment of the Niger Delta
The discovery of crude oil in exportable quantities in Nigeria was greeted with overwhelming ovation and high expectations in terms of the wealth generation possibilities and economic growth for the region. However, 5 decades onwards, the Niger-delta terrain and its people have become disenchanted …
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Application of whole-cell biosensor for the impact of crude oil exploration on the environment of the Niger Delta
The discovery of crude oil in exportable quantities in Nigeria was greeted with overwhelming ovation and high expectations in terms of the wealth generation possibilities and economic growth for the region. However, 5 decades onwards, the Niger-delta terrain and its people have become disenchanted …
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Elf magnetic field influence on ION Channels studied by Patch Clamp Technique: exposure set up and "Whole Cell" measurements on Potassium currents
… magnetic fields on potassium currents in neural cell lines ( Neuroblastoma SK-N-BE ), using the whole-cell Patch Clamp technique. Such technique is a sophisticated tool capable to investigate the electrophysiological activity at a single cell, and even at single channel level. The total potassium …
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Beyond bottlenecks: expression of complementary enzymes and permeabilization of cell membranes to improve performance of CYP153A6 in Escherichia coli whole cell biocatalysis
… out at the millilitre scale, using resting cells. Potential limitations for the whole-cell biocatalyst include insufficient expression of the CYP153A6 or its redox partners, leading to a lack of biocatalytic “active sites”; lack of oxygen for biotransformation and cell metabolism; poor …
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Lab on a chip rare cell isolation platform with dielectrophoretic smart sample focusing, automated whole cell tracking analysis script, and a bioinspired on-chip electroactive polymer micropump
… Contactless DEP (cDEP) is a recently developed cell sorting and isolation technique that uses the DEP force by capacitavely coupling the electrodes across the channel. The cDEP platform sorts cells based on intrinsic biophysical properties, is inexpensive, maintains a sterile environment by …
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Identification of novel gating mechanisms underlying pharmacological potentiation of NMDA receptors
… receptors transiently expressed in HEK 293 cells using whole-cell and single-channel electrophysiology. Only a modest effect on the whole-cell responses was observed by PS in dialyzed (non-perforated) whole-cell recordings. Interestingly, in perforated conditions, PS was found to increase …
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Designing and optimizing tandem bio- and chemo-catalyzed cascade reactions to access industrially relevant chemicals in mild, aqueous conditions
… of non-enzymatic catalysts in conjunction with whole cell biocatalysts. The studies are organized by type of abiotic catalyst and include examples featuring electrocatalysts, photocatalysts, and chemocatalysts. Throughout the review we take note of the challenges faced when interfacing these two …
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On the Modeling of Signaling Networks with Petri Nets
The whole-cell behavior arises from the interplay among signaling, metabolic, and regulatory processes. Proper modeling of the overall function requires accurate interpretations of each component. The highly concurrent nature of the inner-cell interactions motivates the use of Petri nets as a …
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Action of Tyrosyl DNA Phosphodiesterase on 3'-Phosphoglycolate Terminated DNA Strand Breaks
… purified native enzyme (from lymphoblastoid whole-cell extracts using Sephacryl S-300 gel filtration columns), we found that the recombinant enzyme had lesser 3'-PG removal activity than the partially purified native enzyme. On cloning recombinant FLAG-tagged hTdpl into human expression …
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Direct Analysis of Intact Proteins From Microorganisms Using Top Down Mass Spectrometry
… and Saccharomyces cerevisiae. From the whole cell lysate of yeast, ∼120 ion species were successfully identified with 100% sequence coverage, and we also detected several post-translational modifications, including acetylations, phosphorylations, methylations, formylations and …
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Investigating cestode modulation of host neuronal excitability and cell-type-specific gene expression in an in vitro mouse model of neurocysticercosis
… and used singlenucleus RNA sequencing and whole-cell patch-clamp electrophysiology to investigate potential links between inflammation and network excitability at a transcriptomic and electrophysiological level. In the first part of the thesis, I found that while LPS significantly affected …
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