Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 57 for “"whole mount"”.
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Role of DeltaC and DeltaD in Zebrafish Retinal Development
… expression pattern of dlc and dld, determined by whole-mount in situ hybridization, demonstrated that both ligands have similar and distinct expression patterns in WT retinas throughout retinal development. In mutant retinas where dlc and dld are absent, whole-mount in situ hybridization and …
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Untersuchungen zur Bedeutung der sal-ähnlichen Gene bei der Maus
… zu dem humanen SALL1-Gen. Untersuchungen mittels whole mount-in situ-Hybridisierungen zeigten, dass das Expressionsmuster im Mausembryo deutlich mit den beim TBS betroffenen Organen überlappt. Mit einem herkömmlichen knock out-Vektor wurde durch die Klonierung zweier genomischer Fragmente ein …
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From coarse fate choice to precise pattern: post-mitotic progenitor targeting
… movement in non-irradiated planarians. Using whole-mount fluorescence in situ hybridization (FISH) and the quantification of EdU-labeled cells, this study demonstrates that progenitor cells are capable of migrating long distances and exhibit a pronounced anterior bias in their movement and …
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Phenotypic Analysis of Platelets in Patients with Platelet Function Disorders
… and diagnostic usefulness of testing for DGD by whole mount electron microscopy (EM). We found that this test has acceptable performance characteristics, with a within-subject coefficient of variation (CV) of 12% for samples with normal DG counts. Confirmed DGD by whole mount EM showed …
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Imaging Neuromuscular Synaptogenesis
… that precedes synaptogenesis. Here, using whole-mount high-resolution live imaging, we show that glutamatergic synaptogenesis on all muscles in Drosophila embryos is preceded by formation of a myopodial cluster into which PSD-95/Dlg is rapidly recruited. Cell-specific disruption of …
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Functional genomic studies of regeneration in the planarian Schmidtea mediterranea
… Those genes were characterized in silico, then whole-mount in situ hybridizations were performed for each gene. Functional analysis using double-stranded RNA-mediated RNA interference (RNAi) was implemented to determine effects on regeneration. The hypothesis was that effectors of regeneration …
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An investigation into a role for follistatin in early cardiogenesis in Xenopus Laevis
… We have used microarray technology and whole mount in situ hybridization to investigate the mechanism by which RA might alter cardiogenesis. Our results showed that RA inhibits cardiomyocyte differentiation at least partially through increasing follistatin gene expression and decreasing …
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Wnt-/beta- Catenin Zielgene der frühen Mausentwicklung
… exprimiert sind. Durch Expressionsanalysen in Whole- mount in situ- Hybridisierungen sind für drei der Wnt- induzierten Gene spezifische Expressionsmuster in der Mausentwicklung von 7,5- 12,5dpc gezeigt worden.
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Dissecting the molecular mechanisms of germ cell development in the planarian Schmidtea mediterranea
… their expression in planarian germline by whole-mount in situ hybridization (WISH). Finally, we carried out a RNA interference screen to analyze functions of these germline-specific genes in planarian germ cell development. We successfully identified a list of genes, encoding both conserved …
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The Impact of Acetylcholine Receptors on Osteocytes and Bone Mechanoadaptation
… resolution 3D reconstructions of nerve bodies in whole-mount and intact bone specimens. Our results establish a new signaling axis between the brain and bone via the osteocyte with far reaching implications in bone biology which may be vital to treat bone diseases.
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Analyse der differentiellen Expression von Transportfaktoren und deren Funktion bei dem nukleocytoplasmatischen Transport von TFIIIA
… kann. Beide Varianten zeigen in RT-PCR und whole mount" Analysen ein Expressionsprofil, das dem von TFIIIA entspricht.Aufgrund der identischen Expressionsmusterprofile von ribosomalen Proteinen, transportfaktorassoziierten Proteinen und Transportfaktoren wurde die Existenz einer neuen …
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Technological Innovation and Integration of Whole Brain Imaging, Olfactory Stimulation, and Correlative Microscopy in Larval Zebrafish
… presents novel approaches and technologies for whole-brain imaging, olfactory stimulation, and correlative imaging---i.e. the utilization and registration of multiple imaging modalities within a single sample. The overall objective of this thesis research is to not just create technologies, but …
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Isolierung und Charakterisierung des testisspezifisch exprimierten Gens HASH der Maus
… Geweben nachweisbar ist. Mit Hilfe der 'whole mount'-in situ-Hybridisierung konnte nachgewiesen werden, daß das HASH-Gen hauptsächlich im dorsalen Neuralrohr und in den Somiten bei 9.5- und 10.5-Tage alten Embyonen exprimiert ist. Mit Hilfe der RT-PCR und der Northern-Blot-Analysen konnte …
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Gene expression and biochemistry of isoprenoid biosynthesis in the glandular secretory trichomes of Artemisia annua
… gene localization experiments. Traditional and whole-mount in situ hybridization techniques were used to further the study of cell specific gene expression within the glandular trichome. An RNA probe constructed from the sequence of amorpha-4,11-diene synthase localized expression to the 2nd and …
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Integrating genomic, transcriptomic and developmental approaches to investigate coloniality and life cycle evolution in the Hydractiniidae (Hydrozoa: Cnidaria)
… in these key transitions. Furthermore, using whole mount in situ hybridization to characterize the spatial expression of various candidates genes, I validated each approach showing expression consistent with their role in the development of a particular tissue or life cycle stage. Results …
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Cre-lox-basierte Inaktivierung des Pbx3-Gens
… festgestellt werden. In-situ- und „Whole-mount“-in-situ-Hybridisierungen von Wildtyp-Mäusen E 9.5-13.5 zeigten eine besonders starke Expression des Pbx3-Proteins in der Medulla oblongata, dem Hirnstamm und kaudalen Teil des Mittelhirns. Immunhistochemisch waren jedoch keine …
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Investigating the Reconstitution and Function of Peritoneal Cavity Macrophages after LPS-induced Peritonitis
… Spectral flow cytometry and confocal whole mount imaging was used to investigate the ‘disappearance’ of LPM from the peritoneal cavity upon stimulation of the noxious agent. After 10 days of LPS administration, the LPM fate mapping mouse and the Ms4a3-monocyte derived reporter mouse …
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Isolation and Characterization of the Soybean Major Latex Homologue Msg, and Analysis of Its Pod -Specific Promoter
… pods, the Msg transcript was localized by whole mount in situ hybridization to the mid pericarp layer, a continuous single cell layer believed to be involved in pathogen defense. The conclusion is that the Msg promoter is a strong soybean promoter that drives developmentally induced gene …
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Isolation and Characterization of the Soybean Major Latex Homologue Msg, and Analysis of Its Pod-Specific Promoter
… pods, the Msg transcript was localized by whole mount in situ hybridization to the mid pericarp layer, a continuous single cell layer believed to be involved in pathogen defense. The conclusion is that the Msg promoter is a strong soybean promoter that drives developmentally induced gene …
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Spatially precise in situ transcriptomics in intact biological systems
… targeted RNA in situ sequencing was adapted to whole-mount RNA imaging of intact preimplantation mouse embryos. By integrating immunofluorescence and in situ sequencing with live-embryo mechanical measurements, a spatially-resolved multimodal map of preimplantation embryogenesis was generated to …
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