Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 9 of 9 for “"wgbs"”.
-
On the Analysis of DNA Methylation
… one method, whole-genome bisulfite sequencing, WGBS, available to study DNA methylation genome-wide at such high resolution, however its accuracy has not been determined on the scale of individual binding locations. We address this with a two-fold approach. First, we developed an alternative …
-
A sequencing-based analyisis of epigenetic modifications of the mitochondrial genome
… studied using whole genome bisulfite sequencing (WGBS), but recent evidence has uncovered major technical issues, which introduce a potential bias during methylation quantification. In this study, we first validate the technical concerns with WGBS using publicly available datasets. Then we develop …
-
Targeted Epigenetic Editing to Increase Adult Pancreatic Β-Cell Proliferation
… RNA-Seq and whole-genome bisulfite sequencing (WGBS) to profile the aging human β-cell transcriptome and DNA methylome. I found that there are significant changes in gene expression with age, and in DNA methylation, particularly at islet-specific active enhancers. Further, I developed …
-
REDEFINING PROMOTER DNA METHYLATION CHANGE IN CANCER
… a computational method that combines RNA-seq and WGBS data to identify genomic regions where DNA methylation tends to be highly correlated with nearby TSS activity. We refer to these regions as TSS-proximal methylation-controlled regulatory sites (TMRs). We applied Methodical to one normal …
-
The Effects of Cannabis sativa on the Sperm Epigenome
… via whole genome bisulfite sequencing (WGBS) and methylation changes were validated at select genes via bisulfite pyrosequencing. Methylation changes validated in F0 sperm at the gene 2-Phosphoxylose Phosphatase 1 (Pxylp1) were similarly present in F1 sperm, while changes validated in F0 …
-
The evolution of gene regulatory landscapes in mammalian tissues
… I analyzed whole-genome bisulfite sequencing (WGBS) and matched chromatin immunoprecipitation assays followed by sequencing (ChIP-seq) of five TFs from the livers of five mammals. I first characterized DNA methylation patterns at transcription factor binding regions and found four prototypical …
-
Analysis of soybean gene regulation reveals regulatory mechanisms of Rhg1-mediated resistance to soybean cyst nematode
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2019-11-26 without embargo terms
-
DNA methylation: a model system for the study of ageing
DNA methylation is an important epigenetic mark spanning all of life's kingdoms. In humans, DNA methylation has been associated with a wide range of age-related pathologies, including type II diabetes and cancer. More recently, in humans, changes in DNA methylation at specific positions in the …
-
Early Onset Alzheimer’s Disease Markers in Mouse Hippocampus Unveiled by Single-Cell Transcriptomic Analysis Following Cranial Radiotherapy
<p>Cranial radiation therapy plays an integral role in the treatment of brain tumors but can lead to progressive cognitive deficits in survivors by mechanisms that are poorly understood. To develop preventive or mitigative strategies, it is crucial to better understand the underlying pathogenesis …