Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 54 for “"voltage clamp"”.
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Characterization of cardiac IKs channel gating using voltage clamp fluorometry
Voltage-gated ion channels make up a superfamily of membrane proteins involved in selectively or non-selectively conducting charged ions, which can carry current in and out of cells, in response to changes in membrane voltage. Currents carried by ion channels influence the voltage across the cell …
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Der Einfluss lokaler Ionenkonzentrationsänderungen in Membrannähe bei Voltage-Clamp-Messungen am Modellsystem Skelettmuskel
… auf den Stromverlauf bei Voltage-Clamp Experimenten quantitativ zu untersuchen. Als Modellsystem wurde der Skelettmuskel gewählt, wobei zwischen Ca2+-Strömen und K+-Strömen unterschieden wurde, die exemplarisch als Anwendungsbeispiel für physiologisch wichtige Ionenströme …
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Two-Electrode Voltage Clamp (TEVC) Based Scanning Ion Conductance Microscopy: New Methodology and Validation
… microscopy, confocal microscopy, and patch clamping. A key feature of SICM is its non-invasive scanning ability. The ability to scan samples without coming making contact allows for more robust research in biology. Current SICM techniques call for a one electrode probe, but in this research …
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New biophysical methods for the characterization of signal transfer in neurons
… extensive dendritic trees, and therefore somatic voltage clamp of dendritic synapses is often associated with substantial distortion and attenuation of the synaptic currents. A new method is presented which permits faithful extraction of the decay time constant of the synaptic conductance …
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Voltage-Gated Sodium Channel Activity in Mouse Skeletal Muscle Fibers: Normal Gating and Defects Associated with Periodic Paralysis Mutants
… skeletal muscle. We optimized a two-electrode voltage clamp protocol to improve the fidelity of Na+ current recording from acutely dissociated intact muscle fibers. Computer simulation, incorporating measured capacitance and ionic current densities, was used to confirm sufficient voltage …
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Noise Optimization for High-Bandwidth Ion Channel Recordings
… fidelity. This thesis presents an analysis of voltage-clamp current recordings of single ion channels, and concludes that considerable improvements in signal-to-noise ratios can be achieved by minimizing all parasitic capacitances associated with these measurements. A custom integrated …
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The cyclic AMP-activated sodium current in the molluscan neuron: A kinetic analysis of regulation by diffusion, phosphodiesterase and calcium ion
… intracellular iontophoresis of cyclic AMP under voltage clamp condition. The I$\sb{\rm Na,cAMP}$ response to cyclic AMP injection is resistant to protein kinase inhibitors, and is very likely mediated by direct cyclic AMP binding to the channel receptor. The slow I$\sb{\rm Na,cAMP}$ is regulated …
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The Cystic Fibrosis Transmembrane Conductance Regulator: Regulation by HSP-90
… via Ussing chamber analysis, two-electrode voltage clamp analysis, preliminary <em>in vivo</em>animal studies, and protein phosphorylation. The HEK-293 Flag-CFTR cells used in this study allows for a high degree of protein purification in quantities sufficient for mass spectroscopy, which …
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A Study of Ionic Permeability Changes Underlying Excitation in Myelinated Nerve Fibers of the Frog
… Constraint of the membrane potential (the voltage clamp) provided direct measurement of the current carried by the movement of ions through the membrane. By applying operational procedures analogous to those used by Hodgkin and Huxley for the squid axon, the ionic current was resolved into …
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Molecular pharmacology of an insect GABA receptor
… expressed receptors in Xenopus oocytes and used voltage-clamp electrophysiology to detect receptor responses. I carried out computational modelling of these analogues to determine the dipole separation distances and atomic charges. Computational calculations and functional experiments revealed …
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Investigation of an unusual GABA-A receptor: studies on the promiscuous ε [epsilon] subunit
… Xenopus laevis oocytes using the two-electrode voltage-clamp technique. The results obtained confirm that the ε subunit confers unusual properties on the GABAA receptor.
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An examination of interacting residues in the GABA-gated ion channel UNC-49 within the parasitic nematode Haemonchus contortus
… were introduced and analyzed by two-electrode voltage clamp electrophysiology. It was found that both residues are important for receptor function, but modifications to the E183 residue yielded a greater negative impact. It was also found that K181 and E183 are energetically coupled suggesting …
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Acetabularia-Rhodopsin, eine lichtgetriebene Protonenpumpe aus einem autotrophen Eukaryoten
… von Xenopus laevis funktionell exprimiert. Voltage-clamp-Untersuchungen bestätigten den lichtgetriebenen Transport von Protonen durch AR gegen ihren elektrochemischen Gradienten. Der stationäre positive Strom ist spannungsabhängig mit einer sigmoiden I(V)-Kurve. AR ist das erste in einem …
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Adenosinergic modulation of hippocampal gamma oscillations: from single cell to whole animal
… oscillations in vitro. Sharp electrode current clamp and whole-cell voltage clamp experiments showed that \(A_1\)-receptor activation hyperpolarised resting membrane potential, reduced firing rate and EPSP amplitude and shifted the IPSC reversal potential to more negative potentials. Blocking …
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Synthetic Ion Channels From Lipophilic Guanosine Derivatives
… with a bis-lithocholic acid derivative. Voltage clamp experiments demonstrated a series of stable, single ion channel conductances when compound 3-1 was incorporated into a planar phospholipid membrane. These channels are large; with nanoSiemens conductance values and they last for …
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A Pharmacological Analysis of the Ionic Channels of Nerve
… fibers from Rana pipiens were studied with the voltage clamp technique of Frankenhaeuser and Dodge. The observed currents were analysed by a computer in terms of the mathematical model of Hodgkin and Huxley. The sodium, the potassium, and the leakage current components and the time constants …
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Auswirkungen einer kombinierten Hochdruck- und Kühlbehandlung auf die elektrophysiologischen und funktionellen Eigenschaften von Skelettmuskelzellen
… von Membraneigenschaften wurden mit einer Voltage Clamp Technik durchgeführt. In der Erhebung passiver Membraneigenschaften nach verschiedenen Hochdruckbehandlungen konnten druckinduzierte spezifische Veränderungen an der Membran ermittelt werden. Diese druckabhängigen Veränderungen der …
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State-Space Models and Latent Processes in the Statistical Analysis of Neural Data
… compact neuron, given intracellular voltage-clamp or current-clamp recordings from the postsynaptic cell. These methods are based on sequential Monte Carlo techniques ("particle filtering"). We demonstrate, on model data, that these methods can recover the time course of excitatory …
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Gentamicin and FM1-43 enter sensory hair cells through mechanosensory transduction channel
… of evidence extend these conclusions. First, voltage clamp recording confirmed gentamicin entry via the transduction channel: current was diminished but not blocked by the drug, and the block was not enhanced at more negative potentials. Second, injection of FM1-43 into mice labeled many …
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Isolation and partial characterization of three acetylcholine-gated chloride channels in Haemonchus contortus
… analysis of ACC-2 via two-electrode voltage-clamp electrophysiology demonstrated activity of several agonists including carbachol, which elicited a similar response as acetylcholine. Furthermore, in silico protein modelling and agonist docking of all three channels has revealed a …
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