Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"virus-like particles (VLPs)"”.
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The Immunogenicity of Plant-produced Human Papillomavirus (HPV) Virus-like particles (VLPs) in Mice
… by infection with high-risk Human papillomaviruses (HPVs). It is ranked fourth among the top cancers in women worldwide, with ~87% of the global cervical cancer cases reported in developing countries. The HPV L1 capsid protein can self-assemble into virus-like particles (VLPs) that are …
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Development of Virus-like particles (VLPs) Based Vaccines Against Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Porcine Epidemic Diarrhea Virus (PEDV)
Porcine reproductive and respiratory syndrome virus (PRRSV) and porcine epidemic diarrhea virus (PEDV) are two of the most prevalent swine pathogens that have impacted the global swine industry for decades. Both are RNA viruses with increasing heterogeneity over the years, making a vaccine solution …
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Development of candidate Human papillomavirus vaccines
… plant-based vaccines against the Human papillomavirus type 16 (HPV-16), which is primarily responsible for cervical cancer. As a first study, the L1 gene of a South Africna variant of HPV-16 (L1 504) and a mutant (504[ΔA266T]), where the alanine at 266 was mutated to a threonine, were expressed …
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Construction and characterization of chimaeric human immunodefiency virus type 1 subtype C Gag virus-like particles
… making HIV-1 subtype C Pr55gag-based chimaeric virus-like particles (VLPs) as a boost to the HIV-IC multigene DNA vaccine pTHr.grttnC, which encodes a modified Gag-RT-Tat-Nef fusion protein (GRTTN). Furthermore, an attempt was made to produce VLP analogues to the HIV-IA polyepitope DNA vaccine …
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Virus-like particles as a vaccine against porcine reproductive and respiratory syndrome virus
… of new generation PRRS universal vaccines. Virus like particles (VLPs) based vaccines are gaining increasing acceptance compared to subunit vaccines, as they present the antigens in more veritable conformation and are even readily recognized by the immune system. Hepatitis B virus (HBV) core …
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RNA transmission and expression from inert HIV candidate vaccine virus-like-particles
HIV-1 Gag virus-like-particles (VLPs) produced in various expression systems are potent stimulators of both cellular and humoral immune responses in animal models. The encapsidation of large concentrations of random cellular RNA species is known to accompany the assembly of HIV virus particles. …
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"Molecular Analysis of Virulence Factors Unique to Leptopilina heterotoma-a Parasitic Wasp of Drosophila spp."
… the female wasp introduces venom proteins and virus-like particles (VLPs) into developing fly larvae. VLPs are endocytosed by plasmatocytes, a macrophage-like blood cell that is the most abundant hemocyte present in the circulating hemolymph. Once internalized, VLPs destroy plasmatocytes by …
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Design and production of a candidate universal influenza A vaccine in Nicotiana benthamiana plants
The influenza A virus is responsible for 250,000 to 500,000 deaths every year worldwide and millions more could die in the event of a serious pandemic. Vaccines against influenza have existed for long, but until today they have been limited by extensive production times and reduced cross-protection …
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Production, characterization and evaluation of the chemotherapeutic and immunogenic potential of protein based virus-like particles in tumoral and immune cell lines
… to characterize a new doxorubicin (DOX) carrier virus-like platform and to evaluate the cell effects of murine melanoma cells treated with free DOX or carried DOX. It is showed that carried DOX promotes higher cell death rates and enter cells in a different manner whether compared with free DOX. …
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Developing a novel hepatitis B core – based antigen presentation system
… increasing recognition. In parallel, the use of virus-like particles (VLPs) has become standard in vaccine design. The pEAQ-HT vector derived from the cowpea mosaic virus – based CPMV-HT expression system has been shown to allow the production of large amounts of recombinant proteins, including …
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An Investigation into the Use of a Plant-Expressed Virus-Like Particle as an Oral Vaccine Candidate
Abstract Plant-expressed virus-like particles (VLPs) could offer an alternative method for the oral delivery of vaccines. VLPs' robustness, combined with their compartmentalisation within the plant cells, should favour their stability in the harsh gastro-intestinal (GI) environment. In the small …
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A proteomic approach for studying LTR retrotransposon host factors in saccharomyces cerevisae
… are mobile elements that resemble retroviruses, both in their genetic structures and replication cycles. Ty retrotransposition begins in the nucleus with the transcription of Ty RNA. Following translation of Ty proteins, virus-like particles (VLPs) assemble in the cytoplasm. VLPs contain …
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Intranasal Immunization of Pigs with Porcine Reproductive and Respiratory Syndrome Virus like Particles Plus 2’, 3’ –cGamp Vaccigrade™ Adjuvant Exacerbates Viremia After Virus Challenge
<p>Porcine reproductive and respiratory syndrome virus (PRRSV) is an economically devastating virus that causes a persistent infection and spreads quickly. The virus causes abortions in sows and respiratory diseases in young piglets. Current PRRSV vaccines appear to provide limited …
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Development of plant-produced Bluetongue virus vaccines
… domestic and wild ruminants caused by Bluetongue virus (BTV). It has caused several serious outbreaks, the most recent occurring in Northern Europe in 2006 during which high mortality rates of livestock were reported. The only vaccines currently approved and commercially available for use are …
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Expression and characterization of plant produced AHSV nanoparticles encapsulating EGFP
Virus-like particles (VLPs) are virus-based nanoparticles that resemble the native virion but do not contain the viral genome. Heterologous expression of the viral structural proteins results in the spontaneous self-assembly of VLPs that have an empty inner cavity. These particles have an …
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Consistent Fabrication of Ultrasmall PLGA Nanoparticles and their Potential Biomedical Applications
… last few decades. Specifically, ultrasmall nanoparticles, ranging in size from 5 to 50 nm, are highly sought after for their physical and chemical properties and their ability to be easily transmitted though the bloodstream. By adjusting the material properties, size, surface potential, …
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HPV pseudovirion production in plants
Human papilloma virus (HPV) infection is the most common etiological agent of cervical cancer, the most common cancer in women in Africa. The lifecycle of HPV has historically made the virus difficult to culture in vitro, and this has hindered the study of the virus, as well as development of …
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Elucidating the Role of Two Inotropic Agents in Cardiac Hypertrophy: the Ceramides and Apelins
… such as APJ. We discuss the utilization of virus-like particles (VLPs) overexpressing APJ and demonstrate the ability to enrich the binding of positive control apelin, in both simple and complex mixtures containing a mock screening library with over 2500 peptides. The work in this thesis …
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Distribution of Free Marine Viruses of Lower Chesapeake Bay and Their Effects on Life-History Parameters of the Estuarine Copepod <i>Acartia tonsa</i> Dana
<p>Naturally occurring viruses are very abundant in fresh, estuarine, and marine waters, with densities on the order of 10<sup>5</sup>-10<sup>8</sup> viruses ml<sup>-1</sup>. Research has focused on virus effects on bacteria, cyanobacteria, and phytoplankton, as well as mechanisms of virus …
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Plant production of Gonococcal peptide vaccine, candidate peptide display with HPVs
Human Papillomavirus (HPV) and Neisseria gonorrhoeae (Ng) are prominent pathogens responsible for a significant proportion of the global burden attributed to sexually transmitted infections. While vaccines targeting HPV have seen success, an effective vaccine against gonorrhea remains elusive, as …
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