Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"virus induced gene silencing (VIGS)"”.
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Investigating the role of neuropeptides in plant parasitic nematodes
… methodologies available to probe neuropeptide gene function in economically important PPNs. A proof of concept study utilising transgenic Bacillus subtilis to deliver PPN<br/>neuropeptides in a tomato seedling invasion assay identified several neuropeptides which induce aberrant behaviours in …
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Gene discovery in Catharanthus roseus using virus induced gene silencing
This thesis presents the use of Virus Induced Gene Silencing (VIGS) for the discovery of enzymes and transporters involved in monoterpene indole alkaloid (MIA) metabolism in the medicinal plant Catharanthus roseus. C. roseus is the source of a number of MIAs that are used as chemotherapeutic agents …
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Functional Characterization of Monoterpenoid Indole Alkaloid (MIA) Biosynthetic Genes in Catharanthus roseus
… approaches to identify novel MIA biosynthetic genes. Virus-induced gene silencing (VIGS) is being used to screen these candidate genes for their involvement in iridoid biosynthesis pathway, especially in the identification of 7-deoxyloganic acid 7-hydroxylase (CrDL7H) shown by the accumulation …
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The γ-tocopherol-like family of N-methyltransferases: A taxonomically clustered gene family encoding enzymes responsible for N-methylation of monoterpene indole alkaloids
… (NMT). For almost twenty years the gene encoding this NMT had eluded discovery; however, in 2010 Liscombe et al. reported the identification of a γ-tocopherol C-methyltransferase homologue capable of indole N-methylating 2,3-dihydrotabersonine and Virus Induced Gene Silencing (VIGS) …
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Expanding the Role of Oxidoreductases in Benzylisoquinoline Alkaloid Metabolism in Opium Poppy
… the 2-oxoglutarate/Fe(II)-dependent dioxygenase gene family, namely thebaine 6-O-demethylase (T6ODM) and codeine O-demethylase (CODM). When assayed with a wider range of BIAs, CODM, T6ODM, and the functionally unassigned paralogs DIOX2 and DIOX7, renamed protopine O-dealkylase (PODA) and …
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An investigation into the role of ubiquitination in plant immunity
… accumulated reactive oxygen species (ROS), induced the expression of pathogenesis related (PR) genes and developed spontaneous necrotic lesions upon infection with a virulent pathogen. AtPOB1 transcript and protein levels were induced by virulent Pseudomonas syringae. And transient …
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Identication of Candidate Resistance Genes against Rhynchosporium in Barley
… Research was carried out to identify candidate genes for resistance against rhynchosporium in barley, using genetics and next generation sequencing approaches, with the aim of improving our understanding of the molecular mechanisms behind host resistance to rhynchosporium and developing tightly …
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An investigation of gene expression, alternative splicing and nonsense-mediated decay in potato in response to Phytophthora infestans infection
… immunity (ETI). Using a NB-LRR gene enrichment and RNA-seq, previous studies identified over 700 NB-LRR genes in potato.<br/><br/>Dynamic changes in alternative splicing (AS) of NB-LRR genes have been shown in tobacco and Arabidopsis to be essential for the plant response to …
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The Evolution and Development of Nectar Spurs
… is known about how nectar spurs develop, and the genetics underpinning that development remain unexplored in most systems. In the absence of a spurred model organism, this thesis aimed to advance our understanding of nectar spur development in the toadflax genus *Linaria* (Plantaginaceae) – a …
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Functional Characterization of Metallothioneins in Arabidopsis and Barley
… T-DNA insertion mutants lacking 4 MT genes including <em>MT1a, MT2a, MT2b</em> and <em>MT3</em> were developed. The quadruple mutant (<em>mt1a/mt2a/mt2b/mt3</em>) showed no visible phenotype under standard growth conditions. However, it accumulated higher Cu in leaves compared to wild …
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Role of virus genes in seed and aphid transmission and development of a virus-induced gene silencing system to study seed development in soybean
Soybean mosaic virus (SMV) and Tobacco streak virus (TSV) are two important seed transmitted virus that infect soybean. SMV is a member of the family Potyviridae genus Potyvirus, and is transmitted by seed and aphids in soybean. The symptoms of SMV infection on soybean typically include mosaic and …
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Advanced applications of Raman spectroscopy and super-resolution imaging of biological and plant materials
… a developed multimodal imaging method to study gene function in plants, the potential for Raman spectroscopy to infer relative plant metabolite content for optimal harvest, and the prospects of using inorganic semiconductor quantum dots with saturated excitation microscopy for subdiffraction …
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Dissecting key biomass traits through traditional QTL mapping, exploring novel functional genomics approaches, and investigating self-incompatibility in Miscanthus
… are in need of information regarding the genetic architecture of the traits integral to yield and adaptability. To address this, a four year quantitative trait locus (QTL) study was conducted on a full-sib, F1 mapping population of M. sinensis segregating for flowering time, height, leaf …
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Persistent viruses in Capsicum annuum plants: Understanding the mystery guests
Most work on plant viruses focuses on their pathogenicity, but recent studies suggest viruses may benefit their hosts, at least under some conditions. Plant-infecting viruses of the Partitiviridae and Endornaviridae are transmitted only vertically, via pollen and seed, generally cause no disease …