Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 24 for “"virus induced gene silencing"”.
-
Gene discovery in Catharanthus roseus using virus induced gene silencing
This thesis presents the use of Virus Induced Gene Silencing (VIGS) for the discovery of enzymes and transporters involved in monoterpene indole alkaloid (MIA) metabolism in the medicinal plant Catharanthus roseus. C. roseus is the source of a number of MIAs that are used as chemotherapeutic agents …
-
Virus induced gene silencing of <em>Fusarium verticillioides</em> pathogenicity genes in maize
1 PDF file (x, 99 pages)
-
Virus-induced gene silencing and molecular marker mapping for Russian wheat aphid resistance in wheat
To view the abstract, please see the full text of the document.
-
Role of virus genes in seed and aphid transmission and development of a virus-induced gene silencing system to study seed development in soybean
Soybean mosaic virus (SMV) and Tobacco streak virus (TSV) are two important seed transmitted virus that infect soybean. SMV is a member of the family Potyviridae genus Potyvirus, and is transmitted by seed and aphids in soybean. The symptoms of SMV infection on soybean typically include mosaic and …
-
Detection of Acidovorax citrulli, the Causal Agent of Bacterial Fruit Blotch Disease of Cucurbits, Prevention via Seed Treatments and Disease Resistance Genes
… green tea seed treatment, candidate NB-LRR genes for disease resistance, and optimization of virus induced gene silencing for melon and watermelon crops. The potential application of attenuated total reflectance (ATR) Fourier transform infrared spectroscopy (ATR-FTIR) and high-resolution …
-
The use of reverse genetics to investigate plant-parasite interactions
… demonstrates the development and use of a novel Virus Induced Gene Silencing platform to investigate the interactions of plants and parasites. Host and parasite genes are targeted using this technology which when silenced indicate their involvement in establishing pathogenicity. The several …
-
Investigating the role of neuropeptides in plant parasitic nematodes
… methodologies available to probe neuropeptide gene function in economically important PPNs. A proof of concept study utilising transgenic Bacillus subtilis to deliver PPN<br/>neuropeptides in a tomato seedling invasion assay identified several neuropeptides which induce aberrant behaviours in …
-
Improving the thermal tolerance of photosynthesis in wheat
… in the wheat lines investigated. Two genes encoding Rca in wheat were identified; one gene is alternatively spliced to produce α and β isoforms. Virus-Induced Gene Silencing of the Rca isoforms in wheat indicated that the Rca genes in wheat may be co-regulated. A non-radioactive …
-
Functional Characterization of Monoterpenoid Indole Alkaloid (MIA) Biosynthetic Genes in Catharanthus roseus
… approaches to identify novel MIA biosynthetic genes. Virus-induced gene silencing (VIGS) is being used to screen these candidate genes for their involvement in iridoid biosynthesis pathway, especially in the identification of 7-deoxyloganic acid 7-hydroxylase (CrDL7H) shown by the accumulation …
-
The γ-tocopherol-like family of N-methyltransferases: A taxonomically clustered gene family encoding enzymes responsible for N-methylation of monoterpene indole alkaloids
… (NMT). For almost twenty years the gene encoding this NMT had eluded discovery; however, in 2010 Liscombe et al. reported the identification of a γ-tocopherol C-methyltransferase homologue capable of indole N-methylating 2,3-dihydrotabersonine and Virus Induced Gene Silencing (VIGS) …
-
Expanding the Role of Oxidoreductases in Benzylisoquinoline Alkaloid Metabolism in Opium Poppy
… the 2-oxoglutarate/Fe(II)-dependent dioxygenase gene family, namely thebaine 6-O-demethylase (T6ODM) and codeine O-demethylase (CODM). When assayed with a wider range of BIAs, CODM, T6ODM, and the functionally unassigned paralogs DIOX2 and DIOX7, renamed protopine O-dealkylase (PODA) and …
-
Sequence-specific gene silencing by transitive signals in plants
… field that aims at unraveling the process of RNA silencing, in particular of transitive silencing. In the first, application-oriented part of the thesis, we examined the potential of transitive silencing as a tool for functional genomics, and in the second, more fundamental part, we focused on …
-
Persistent viruses in Capsicum annuum plants: Understanding the mystery guests
Most work on plant viruses focuses on their pathogenicity, but recent studies suggest viruses may benefit their hosts, at least under some conditions. Plant-infecting viruses of the Partitiviridae and Endornaviridae are transmitted only vertically, via pollen and seed, generally cause no disease …
-
An investigation into the role of ubiquitination in plant immunity
… accumulated reactive oxygen species (ROS), induced the expression of pathogenesis related (PR) genes and developed spontaneous necrotic lesions upon infection with a virulent pathogen. AtPOB1 transcript and protein levels were induced by virulent Pseudomonas syringae. And transient …
-
Identication of Candidate Resistance Genes against Rhynchosporium in Barley
… Research was carried out to identify candidate genes for resistance against rhynchosporium in barley, using genetics and next generation sequencing approaches, with the aim of improving our understanding of the molecular mechanisms behind host resistance to rhynchosporium and developing tightly …
-
An investigation of gene expression, alternative splicing and nonsense-mediated decay in potato in response to Phytophthora infestans infection
… immunity (ETI). Using a NB-LRR gene enrichment and RNA-seq, previous studies identified over 700 NB-LRR genes in potato.<br/><br/>Dynamic changes in alternative splicing (AS) of NB-LRR genes have been shown in tobacco and Arabidopsis to be essential for the plant response to …
-
The Evolution and Development of Nectar Spurs
… is known about how nectar spurs develop, and the genetics underpinning that development remain unexplored in most systems. In the absence of a spurred model organism, this thesis aimed to advance our understanding of nectar spur development in the toadflax genus *Linaria* (Plantaginaceae) – a …
-
Functional Characterization of Metallothioneins in Arabidopsis and Barley
… T-DNA insertion mutants lacking 4 MT genes including <em>MT1a, MT2a, MT2b</em> and <em>MT3</em> were developed. The quadruple mutant (<em>mt1a/mt2a/mt2b/mt3</em>) showed no visible phenotype under standard growth conditions. However, it accumulated higher Cu in leaves compared to wild …
-
Maize R gene Rxo1 Confers Disease Resistance on Pepper and Nicotiana benthamiana
… destructive diseases on pepper. Due to limited genetic resistance resources in host species, a promising strategy for controlling BS disease is to transfer nonhost disease resistance (R) genes from other plant species into pepper plants to confer broad-spectrum and durable resistance. A maize R …
Page 1 of 2