Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 41 for “"virus entry"”.
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USING SINGLE VIRION FUSION ASSAY TO STUDY INFLUENZA VIRUS ENTRY
Understanding the mechanism of influenza virus entry is critical for effectively developing anti-viral drugs and vaccines. The entry of influenza virus is mediated by two proteins: hemagglutinin (HA) and neuraminidase (NA). During virus infection, HA first binds to sialic acid moieties on …
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Molecular basis oh herpes simplex virus entry into the cell and retargeting of the viral tropism for the design of oncolytic herpesviruses
Herpes simplex virus 1 (HSV-1) infects oral epitelial cells, then spreads to the nerve endings and estabilishes latency in sensory ganglia, from where it may, or may not reactivate. Diseases caused by virus reactivation include mild diseases such as muco-cutaneous lesions, and more severe, and even …
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Real Time PCR-Based Infectivity Assay and Characterization of Cell Surface Receptors for Turkey Hemorrhagic Enteritis Virus
Turkey hemorrhagic enteritis virus (THEV) is responsible for the hemorrhagic enteritis (HE) disease in commercial turkeys through infections by its virulent strains. HE is an acute condition characterized by depression, immunosuppression, bloody droppings, intestinal hemorrhage, and death. THEV …
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Dissecting the Entry Pathways of Hepatitis C and Bovine Viral Diarrhea Viruses
<p>Hepatitis C virus (HCV) and bovine viral diarrhea virus (BVDV) are closely related members of the family Flaviviridae and are important human and animal pathogens, respectively. In this work, I investigated how these viruses interact with and enter both naïve and previously infected cells, …
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Interazioni tra Glicoproteine che mediano la penetrazione del Virus Herpes Simplex nella cellula e disegno razionale di Peptidi Inibitori
Herpes simplex virus entry into cells requires a multipartite fusion apparatus made of gD, gB and heterodimer gH/gL. gD serves as receptor-binding glycoprotein and trigger of fusion; its ectodomain is organized in a N-terminal domain carrying the receptor-binding sites, and a C-terminal domain …
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Investigation of the role of target cell factors in retrovirus transduction
… specificity of gene transfer of recombinant retroviruses. Successful gene transfer by recombinant retroviruses is a multi-step process and we have focused our efforts on those target cell factors that affect virus entry into the target cell. We have developed an experimental system to study the …
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PROTEASOME-DEPENDENT ENTRY OF HERPES SIMPLEX VIRUS
Herpes simplex virus entry into cells is a multistep process that engages the host cell machinery. The proteasome is a large, ATP-dependent, multisubunit protease that plays a critical role in the maintenance of cell homeostasis. A battery of assays were used to demonstrate that proteasome …
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Molecular genetics and functions of Kaposi's sarcoma-associated herpesvirus glycoproteins in viral entry and virus-induced cell fusion
Kaposi’s sarcoma-associated herpesvirus (KSHV) is considered the etiologic agent of Kaposi’s sarcoma and several lymphoproliferative disorders. Recently, the full-length KSHV genome has been cloned into a bacterial artificial chromosome (BAC) and successfully recovered in 293 cells. The herpesviral …
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Analysis of the role of lipids in retrovirus transduction
… used in gene therapy protocols are mammalian virus vectors. Specifically, retroviruses are one of the most common viral vectors used since they are able to permanently integrate their transgene into the host cell genome, providing, in principal, to a long-term cure. The potential applications …
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Efforts towards the synthesis of fully N-differentiated heparin-like glycosaminoglycans; and, Investigations into the mechanism of inactivation of RTPR by gemcitabine triphosphate
… in interactions with growth factors, cytokines, virus entry, and other functions. Currently, no general synthesis of arbitrary HLGAG sequences has been demonstrated. The modular synthesis of glycosaminoglycans requires straightforward methods for the production of large quantities of protected …
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Enhancement Of Ebola Virus Infection By Seminal Amyloid Fibrils
… West Africa epidemic was the worst Ebola virus outbreak on record, with the infected numbering over 28,000 and the dead more than 11,000. Among those who survived the disease, Ebola virus was found to persist in numerous anatomical locations and be shed in semen. During the West Africa …
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Defining the mechanism(s) of Hepatitis C virus (HCV) entry
Hepatitis C virus (HCV) is a major human pathogen and the leading cause of cirrhosis and liver cancer worldwide. HCV entry is clathrin- and pH-dependent, and requires CD81, Scavenger receptor BI (SR-BI), and the tight junction (TJ) proteins Claudin-1 and Occludin. Primary HCV strains cannot be …
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Development and Characterization of A Recombinant Orf Virus Vector Expressing the Spike Protein of Porcine Epidemic Diarrhea Virus
<p>Orf virus (ORFV), the type member of the genus <em>Parapoxvirus</em> of the family <em>Poxviridae</em>, causes orf or cutaneous pustular dermatitis in sheep and goats. ORFV is a ubiquitous virus capable of re-infecting its hosts multiple times over time. ORFV causes a non-systemic, self-limiting …
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The Role Of The Membrane Proximal Region Of The M2 Cytoplasmic Tail In Virus Replication
Influenza A virus encodes M2, a proton channel that has been shown to be important during virus entry and assembly. The primary aim of this thesis was to investigate the role of the membrane proximal region, residues 46-69, of the M2 cytoplasmic tail during virus replication. A cholesterol …
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Chemical Tools for Exploring IFITM3 S-Palmitoylation and Mechanism
… restrict the infection of multiple pathogenic viruses. Initial studies by our laboratory showed that S-fatty-acylation on three conserved Cys residues were crucial for IFITM3 anti-influenza virus activity. However, endogenous levels and site-specific functions of S-palmitoylation on the …
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Host Entry Factors for Human Coronaviruses
<p>Coronaviruses infect a diverse range of animals from birds to pigs and cats to humans. Coronaviruses employ RNA-based replicative processes and as such are genetically adaptable to acquire novel host ranges. A coronavirus from one species can jump to another by shifting its entry requirements. …
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Viral and cellular proteins involved in vaccinia virus egress
Vaccinia virus (VACV) is a large double-stranded DNA virus with a cytoplasmic site of replication. It has a complex life cycle that produces two distinct infectious virion forms, Intracellular Mature Virions (IMVs) and Extracellular Enveloped Virions (EEVs). The host cell microtubule trafficking …
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Cryo-electron microscopy of HPV16 pseudovirions reveal changes in capsid conformation upon furin cleavage
Persistent infection by oncogenic human papillomavirus (HPV) is the primary cause of cervical cancer, a leading cause of cancer deaths in women worldwide. There are no treatments for HPV infection, and although prophylactic vaccines are effective and safe, they are HPV type specific, provide little …
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Characterization of structure and dynamics of membrane proteins from solid-state NMR
… and C-terminal cytoplasmic domain of influenza A virus M2 and 2) HIV-1 glycoprotein gp4l membrane-proximal external region and transmembrane domain (MPER-TMD) in a near native membrane environment. The cytoplasmic domain of M2 is necessary for membrane scission and virus shedding. The M2(22-71) …
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Autonome Parvoviren
… in adult animals make the two rodent parvoviruses MVMp and H1 promising vectors for cancer gene therapy. Recombinant vectors were developed from genomes of these viruses in which a part of the capsid-protein-coding sequence was replaced by a transgene. These recombinant viruses can …
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