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Applying single-molecule localisation microscopy to achieve virtual optical sectioning and study T-cell activation

Palayret, Matthieu Grégoire SimonUniversity of Cambridge2015

… fluorophores that emit from above or below a virtual ‘light-sheet’, a thin volume centred on the focal plane of the microscope. This method provides qualitative and quantitative improvements to SMLM. In the Part B of this thesis, SMLM is applied to study T cell activation. Although the T cell …

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