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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 139 for “"virion"”.
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USING SINGLE VIRION FUSION ASSAY TO STUDY INFLUENZA VIRUS ENTRY
… viral binding and membrane fusion at the single-virion level to obtain high resolution measurements of each of these processes in vitro. SPT combines total internal reflection fluorescence (TIRF) microscopy with microfluidics and supported lipid bilayers, making it a very powerful tool for …
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Mechanism and Evolutionary Origins of HIV-1 Virion Entrapment by Tetherin
… cell surface. However, the mechanisms underlying virion retention have not yet been fully delineated. In this body of work, we employed biochemical assays and engineered tetherin proteins to demonstrate conclusively that virion tethers are composed of the tetherin protein itself, and to elucidate …
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Structural Studies On The Rubella Virus Capsid Protein And Its Organization In The Virion
… protein is a critical structural component of virions as well as a key factor in virus-host interactions. Three crystal structures of the structural domain of the rubella capsid protein have been described here. The polypeptide fold of the capsid protomer has not been observed previously. The …
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The Relationships of Some Defective Interfering Particles to The Infectious Virion of Vesicular Stomatitis Virus by Analysis of The Annealing Properties of Their Ribonucleic Acid
Made available in DSpace on 2015-05-14T14:56:19Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 7412081.PDF: 2956918 bytes, checksum: 97928475ba6583a7282ceab4ab227b97 (MD5) Previous issue date: 1973
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Genetic and Biochemical Analyses of the Flaviviridae Capsid Proteins
… with envelope glycoproteins. The process of virion morphogenesis is not well understood, but the presumed involvement of numerous viral and cellular components makes it an attractive target for novel therapeutic drug design. To investigate the early events of Flaviviridae particle assembly, …
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Hepatitis B Virion and cccDNA Formation in Primary Tupaia Hepatocytes and Human Hepatoma Cell Lines upon HBV Genome Transduction with Replication-defective Adenovirus Vectors and in vivo Infection of Tupaias
… nucleocapsids, and enveloped virions. It was further demonstrated that covalently closed circular HBV DNA is formed. Therefore, primary tupaia hepatocytes support all steps of HBV replication following deposition of the genome in the nucleus, including the intracellular …
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Structural and Functional Requirements for Homotypic and Heterotypic Interference by Indiana Serotype Defective Interfering Particles of Vesicular Stomatitis Virus
… whose genomes mapped at the 5' end of the virion RNA, this target size corresponded to the entire DI particle RNA molecule regardless of whether it amounted to 10%, 30%, or 50% of the viral genome. These data were interpreted as demonstrating that both termini of the DI particle RNAs were …
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Site-Specific Labelling of the Flavivirus Envelope Protein using Genetic Code Expansion
… The E proteins arranged on the surface of the virion are not static and pose a dynamic landscape for recognition by host factors, including antibodies. Many antibodies bind virion surfaces not predicted to be accessible using static models, yet neutralise infection by binding epitopes exposed …
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HEPATITIS A VIRUS: GROWTH CHARACTERISTICS, PURIFICATION, AND CAPSID GENE ORDER (PEPTIDES, IMMUNOREACTIVITIES, POLYPEPTIDES).
… one-step growth curve was determined. Detectable virion production was absent for approximately 20 h post-infection (p.i.) and was followed by a 4-day logarithmic phase of virus production. A maximum intracellular virus titer of 10⁹ radioimmunofocus-forming units (RFU) per milliliter was achieved …
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PROTEASOME-DEPENDENT ENTRY OF HERPES SIMPLEX VIRUS
… properties of ICP0 that is present in the virion tegument layer have not been well characterized. For these reasons, I sought to characterize tegument ICP0 and determine the role of tegument ICP0 during proteasome-dependent entry of herpes simplex virus. Protein compositions of wild-type …
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Molecular cloning and analysis of the genome of bovine parvovirus
… cloned by blunt end ligation of double-stranded virion DNA into the plasmid pUC8. The resulting genomic clones were infectious after transfection into bovine fetal lung (BFL) cells. Sequencing of the plasmids demonstrated that deletions were common at both ends of the cloned BPV genome. Deletions …
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Human Cytomegalovirus Us17 Locus Fine-Tunes Innate And Intrinsic Immune Responses
… one member, US17, plays an important role during virion maturation. Microarray analysis of cells infected with a recombinant HCMV deleted for US17 (ΔUS17) revealed blunted host innate and interferon responses at early times after infection (12 hpi), a pattern opposite that previously seen in the …
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Genetic and biochemical studies of the lipid-containing bacteriophage PR4
… coli and Salmonella typhimurium. The icosahedral virion consists of an external protein capsid layer which surrounds a membrane vesicle enclosed ds DNA genome.
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Architecture and assembly of maize streak virus: insights from 3D electron microscopy
… shares a unique twinned icosahedral ("geminate") virion architecture (22 x 38 nm) with all other family members. Geminate particles consist of 110 coat protein (CP) subunits that assemble onto a circular single-stranded DNA (ssDNA) genome. Each T= I unit is an incomplete icosahedron assembled from …
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INTERACTIONS BETWEEN THE GLYCOSYLATED GAG PROTEIN OF A MURINE LEUKEMIA VIRUS AND MURINE APOBEC3: NOVEL INSIGHTS INTO HOW A MURINE LEUKEMIA VIRUS COUNTERACTS A RESTRICTION FACTOR
… gGag is protective against both cellular and virion-associated mA3 in vitro and is protective against mA3 in vivo. While there was no direct interaction between mA3 and gGag in an infected cell, gGag and mA3 are localized in the same compartment in the virion and are able to be coprecipitated …
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INTERACTIONS BETWEEN THE GLYCOSYLATED GAG PROTEIN OF A MURINE LEUKEMIA VIRUS AND MURINE APOBEC3: NOVEL INSIGHTS INTO HOW A MURINE LEUKEMIA VIRUS COUNTERACTS A RESTRICTION FACTOR
… gGag is protective against both cellular and virion-associated mA3 in vitro and is protective against mA3 in vivo. While there was no direct interaction between mA3 and gGag in an infected cell, gGag and mA3 are localized in the same compartment in the virion and are able to be coprecipitated …
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