Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"viral polymerase"”.
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Mechanistic analysis of the Zika virus translation-replication switch
… This facilitates the translocation of the viral polymerase NS5 from its site of recruitment at the 5′ end of the genome to the 3′ end in order to begin negative-strand RNA synthesis. However, it is unknown how the switch from genome translation to genome replication during flavivirus …
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The Role of NS1-BP in Influenza Virus Replication
… export, and cells become highly permissive to viral replication. Another intranuclear pool of NS1 was found to interact with a host protein termed NS1-BP, which has been suggested to play a role in pre-mRNA splicing. However, the functions and mechanisms of NS1-BP involved in influenza life …
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Proviral Roles of Ebola virus VP35 Ubiquitination
… type I interferon pathway to acting as the polymerase co-factor. The mechanisms that regulate which function any given VP35 molecule engages remains unknown. Previously, we observed the host E3 ubiquitin ligase TRIM6 conjugates ubiquitin onto VP35 at lysine (K) 309. This post-translational …
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Investigating the mechanisms behind the immunostimulatory activity of influenza A virus RNA polymerase mutations
… essential for the development of protective antiviral responses. However, uncontrolled release of proinflammatory cytokines is also associated with severe and fatal disease caused by highly pathogenic avian and pandemic IAV strains. IAV infections are primarily detected by the retinoic …
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The Role of ORF59 and PAN ENE in Viral Replication of KSHV
… malignancies. During reactivation, multiple viral and cellular factors are required to ensure progression through the KSHV viral lytic cycle. One of these factors is the viral polymerase processivity factor ORF59. The main functions of ORF59 are to localize the viral polymerase to the nucleus …
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Phase Separation Mediated Compartmentalisation of Rotavirus Replication
… that function as sites of genome replication and viral particle assembly in rotaviruses. Intrinsically disordered proteins are key drivers of LLPS. In rotaviruses, the disordered protein NSP5 binds the RNA chaperone NSP2, viral RNAs and other viral proteins to form replication factories known as …
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MicroRNA-122 and Poly(C)-Binding Protein 2 Bind to the Hepatitis C Virus Genome, Regulating Viral Replication and Genome Structure
… genome which is used as the template for both viral translation and negative-strand RNA synthesis. As both processes work in opposite directions on the genome the virus requires a mechanism to coordinate what process is acting on a given copy of genome. It has been suggested that the host …
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Genetic Determinants of Junin Virus Attenuation
… and functional characterization of viral genetic factors implicated in JUNV pathogenesis or attenuation would significantly improve the understanding of the molecular mechanisms underlying AHF and facilitate the development of novel, effective and safe vaccines. To this end, an RNA …
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High-throughput sequencing of RNA 5'- and 3'-termini yields insights into viral and vertebrate gene expression
… RNAs. In a process termed cap snatching, the viral polymerase cleaves host RNAs ~10-13 nucleotides downstream of their caps and uses the resulting fragments to prime viral transcription. High-throughput 5' rapid amplification of cDNA ends resulted in 54 million chimeric reads containing …
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A study of norovirus: molecular epidemiology, pathogenesis and antiviral development
… due to the lack of effective vaccine or antiviral. This is partly due to limitations in propagating human NoV in cell culture, which has hampered the study of NoV biology, antiviral development and pathogenesis. Another aim of this thesis was to understand how MNV infection avoids the host …
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Influenza Viral Protein Interaction Detecting Methods Development and A.M1-host Interplay Network Investigation
<p>The viral RNA-dependent RNA polymerases of influenza A and B are trimetric complexes (PA, PB1, and PB2 subunits), and are crucial for both transcription and replication of the viral genome. While there are extensive data on the structure and function of influenza A polymerase, the influenza B …
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Roles of Interferon Lambda on Hepatitis B Virus Replication
… (IFN-α) and the nucleosidic inhibitors of the viral polymerase, lamivudine and adefovir, are approved for therapy. However, these therapies are limited by the side effects of interferon and by the substantial resistance of the virus to nucleosidic inhibitors. New antiviral molecules suitable …
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Characterisation of the Mechanism of Norovirus VPg-Nucleotidylylation
… the age of 5. Despite causing most cases of viral gastroenteritis, noroviruses remain poorly characterised compared to other viruses. Infectious norovirus RNA is covalently linked to VPg (viral protein genome-linked) at the 5’ end of the genome through VPg-mediated RNA priming of viral RNA …
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Localization of Red Clover Necrotic Mosaic Virus Polymerase Proteins at the Endoplasmic Reticulum of Living Plant Cells
… motifs characteristic of RNA-dependent RNA polymerases, and while the function of p27 is unclear, it is very likely to be part of the RCNMV replication complex. I have investigated the localization of the RCNMV replicase proteins in plants using green fluorescent protein (GFP) fusions …
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Molecular biology of segment 8 of the influenza A virus genome
… new gene product and 2) interactions of the viral non-structural protein translated from this segment – NS1 – with a host cell protein during infection. 1) The genome of influenza A virus is packaged into virions as negative sense viral RNA. To date, all known viral proteins are encoded in …