Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"viral RNA synthesis"”.
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Genetic Determinants of Junin Virus Attenuation
… and functional characterization of viral genetic factors implicated in JUNV pathogenesis or attenuation would significantly improve the understanding of the molecular mechanisms underlying AHF and facilitate the development of novel, effective and safe vaccines. To this end, an RNA …
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CHARACTERIZATION OF INFLUENZA NUCLEOPROTEIN BODY DOMAIN AS ANTIVIRAL TARGET
<p>Influenza is a segmented negative strand RNA virus. Each RNA segment is encapsulated by viral nucleoprotein (NP) and bound by the viral RNA dependent RNA polymerase (RdRP) to form viral ribonucleoproteins (vRNPs) responsible for RNA synthesis. NP is a structural component of the vRNP but also …
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Poliovirus Replication: Interactions with Cytoplasmic Membranes and with Compounds Which Affect Virus Biosynthesis
<p>Picornavirus multiplication is intimately associated with intracellular membranes and affects their production and distribution within the cell. We have shown that the density distribution of cytoplasmic membranes separated by isopycnic centrifugation in discontinuous sucrose gradients is …
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Investigating the mechanisms behind the immunostimulatory activity of influenza A virus RNA polymerase mutations
… essential for the development of protective antiviral responses. However, uncontrolled release of proinflammatory cytokines is also associated with severe and fatal disease caused by highly pathogenic avian and pandemic IAV strains. IAV infections are primarily detected by the retinoic …
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The Influenza A Virus NS1 Protein and Vriral mRNA Nuclear Export
… cell nucleus; a feature which means both the viral genome (vRNA) and mRNA must be exported from the nucleus to the cytoplasm. The mechanism by which vRNA nuclear export is achieved has been well characterised, but how viral mRNAs are exported is poorly understood. The cellular NXF1-dependent …
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Impact of mutations in non-structural proteins on SARS-CoV-2 replication
… and functional implications of mutations in viral proteins. The prevailing belief is that SARS-CoV-2 originated from a zoonotic transmission involving bats as the natural reservoir hosts, with an unknown intermediate host facilitating transmission to humans. Genomic sequencing and …
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The Diversion of Macromolecular Synthesis in L-Cells Towards Ends Dictated by Mengovirus
… last few years. In the case of the details of viral nucleic acid metabolism, the pioneering work of S.S. Cohen and A. Kornberg and the many studies which followed on bacterial viruses have provided much knowledge about the enzymes involved in bacteriophage multiplication and the physiological …
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Characterisation of the Mechanism of Norovirus VPg-Nucleotidylylation
… the age of 5. Despite causing most cases of viral gastroenteritis, noroviruses remain poorly characterised compared to other viruses. Infectious norovirus RNA is covalently linked to VPg (viral protein genome-linked) at the 5’ end of the genome through VPg-mediated RNA priming of viral RNA …
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Structures, Functions, and Druggability of the SARS-CoV-2 Replication–Transcription Complex
… broadly related CoVs. An essential aspect of viral propagation is the replication process in which viral enzymes orchestrate the production of nascent viral RNA genomes. CoV RNA synthesis is mediated by the RNA-dependent RNA polymerase (RdRp) which requires a coterie of viral nucleic …
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Understanding the Role of the Hypervariable Region in the Open Reading Frame 1 of the Hepatitis E virus in Viral Replication
… a major cause of enterically transmitted acute viral hepatitis in developing countries that lack proper hygienic infrastructure. Hepatitis E is globally distributed and has emerged as an important public health disease in both developing and industrialized countries. HEV is a non-enveloped virus …
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Biochemical and Biological Studies on the Growth of an RNA Containing Bacteriophage
<p>The growth of the RNA-containing bacteriophage f2 has been studied. DNA synthesis appears normal after phage infection. It is possible to dissociate the synthesis of viral RNA from the synthesis of DNA using thymine-less bacteria with fluorinated pyrimidines and Mitomycin C as inhibitors of DNA …
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The contribution of viral and host cell factors to replication of the hepatitis C virus RNA genome
… in vitro systems that permit replication of the viral RNA genome and virus particle production. However, the exact functions of the viral proteins, particularly those engaged in RNA synthesis, are poorly understood. It is thought that NS4B, one of the replicase components, induces the formation …