Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"vesicular transport"”.
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Evolution of vesicular transport in kinetoplastids: dynamics and novel gene products
The membrane trafficking system mediates delivery of macromolecules and metabolites to discrete intracellular compartments from their site of uptake or synthesis. For many pathogens the trafficking system has a special relevance as it is responsible for maintaining the host-pathogen interface, …
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Analysis of Hepatocyte Secretion Pathways: A Case Study on Hepatic Apolipoproteins, Serum Albumin, and Hepatitis C Virus
… in protein secretion, lipid storage and transport, and excretion. Some of these functions are carried out via polarized secretion of simple protein cargo, such as serum albumin, or large macromolecular lipid-protein complexes, the lipoproteins. The hepatocyte is also the site of infection …
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The Role of ERp57 in Hras Intracellular Trafficking and Function.
… membrane association is mediated by vectorial vesicular transport from the endomembrane system. ERp57, an ER chaperone, has been shown to specifically bind farnesylated Hras but not non-farnesylated Hras. The objective of this study was to determine if ERp57 participates in Ras trafficking and …
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Regulation of p190RhoGEF by Activated Rho and Rac GTPases: Amplification and Crosstalk
… motility, cell division, gene transcription, vesicular transport, and various enzymatic activities. Activation of Rho proteins largely depends on Rho Guanine nucleotide Exchange Factors (RhoGEFs), which catalyze the exchange of GTP for GDP on Rho. For classical RhoGEFs, the exchange activity …
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The Conserved Oligomeric Golgi (COG) Complex Is Required for Normal Import of Fatty Acids in Saccharomyces Cerevisiae
… in that work. Cog7p functions in intra-Golgi vesicular transport in concert with seven other proteins. This protein complex is found in both yeast and mammals. We found Cog7p in a different context through a screen to identify proteins that function in the trafficking of membrane proteins to …
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Structural Analysis of Cycle Inhibiting Factor from Pathogenic Escherichia Coli
… host functions such as cytoskeleton assembly, vesicular transport, and apoptosis are numerous (Galan and Wolf-Watz, 2006). However, until recently, less progress had been made with bacteria that also possess virulence mechanisms that target the host cell cycle. Studies have demonstrated the …
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Illuminating Organellar and Molecular Organization of the Endomembrane System
… system communicate with each other through vesicular transport as well as direct membrane-membrane contact. In this work, I first probe spatial organization of membrane contact sites between the endoplasmic reticulum and the plasma membrane in mammalian cells. These membrane contact sites, …
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The SAC1p, an integral membrane protein involved in secretory pathway function and actin function in Saccharomyces cerevisiae
… cells. Proteins destined for secretion are transported in a vectorial fashion from the endoplasmic reticulum to the Golgi apparatus to the cell surface. Superimposed on the normal flow of secretory traffic in yeast is a level of spatial organization. Golgi-derived secretory vesicles are …
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β-cell dysfunction in Huntington's disease
… cell dysfunction in our model. Next we analyzed vesicular trafficking as this has been found aberrant in HD and is vital for sustained insulin release. These studies showed that the transport of insulin granules along microtubule filaments is perturbed. Furthermore, we found that mutant …
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Studien zum endosomalen Transport und der Signaltransduktion des Nervenwachstumsfaktors NGF unter physiologischen und pathophysiologischen Bedingungen
… within the cell. The neuro-trophic signal is transported in so called “signalling endosomes” which are retro-gradely transported over long distances from synapses to neuronal cell bodies. Sorting and transport defects severely impair the viability of neurons since the growth factors may need …
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Lipid rafts in protein sorting and yeast cell polarity
… This organelle sorts proteins and lipids into vesicular transport carriers that are targeted via different pathways to distinct membrane compartments of the cell. The molecular principles that operate in cargo sorting at the TGN are still not very well understood. Especially, we know very …
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Steps to Reconstitute in vitro a Complete Round of COPI vesicle Budding, Uncoating and Fusion
… COPI coated vesicles), COPI vesicles mediate the transport in both anterograde and retrograde transport within the Golgi complex (Orci et al., 1997) as well as recycling of proteins from the Golgi to the ER (Cosson and Letourneur, 1994; Letourneur et al., 1994). An in vitro based assay using …
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Role of Membrane Contact Sites in the Neuropathogenesis of GM1-Gangliosidosis
… such as Ca2+, between the organelles via non-vesicular transport. The two most studied MCS are those formed between the ER and mitochondria, known as mitochondrial-associated ER microdomains, or MAM, and those between the ER and PM, known as ER-PM junctions. These MCS are important for …
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Mechanisms of regulation of islet function by nadph oxidase
… fusion and release of insulin into circulation. Vesicular transport and fusion events are tightly regulated by signals which coordinate between vesicle- and membrane-associated docking proteins. It is now being accepted that reactive oxygen species [ROS] plays a second messenger role in islet …
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Gold Nanoparticles as a Delivery System of Oligonucleotides into the Brain
… demonstrated that both active and passive transport mechanisms were involved in the uptake of these nanoparticles. However, knockdown of the insulin-receptor on the endothelium did not reduce transport of insulin-coated nanoparticles. It appeared that the OEG-amine coating alone induced …
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The Sec6/8 (a.k.a. Exocyst) Complex Supports DNA Repair Fidelity
The exocyst complex, first described in yeast, is a heterooctomeric complex that serves as a signaling platform to mediate cellular responses to diverse spatial and temporal cues. Evidence suggests that the exocyst might contribute to oncogenesis, potentially by disrupting spatial and temporal …
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Characterization of Class D VPS Proteins
… classes of mutants, each defective in one transport step in the VPS pathway. The Class D VPS proteins are thought to control anterograde traffic between the late Golgi and late endosome. Although most of these proteins have homologues of known function in other systems, two exceptions are …
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PI(4)P-Dependent Recruitment of Clathrin Adaptors to the Trans-Golgi Network
The Trans Golgi Network (TGN) is the cell's central sorting station, and the complex trafficking patterns are organized by many types of trafficking adaptors. These include the heterotetrameric adaptor protein complexes (APs) and the monomeric Golgi-localized, gamma-ear containing, Arf-binding …
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Structural Studies of Complexin/SNARE Interactions
Vesicular neurotransmitter release is mediated by exocytosis of synaptic vesicles at the presynaptic active zone of nerve terminals. The Ca2+-triggered release process is extremely fast, lasts less than half a millisecond, and is tightly regulated by Ca2+. Action potentials cause Ca2+ influx …
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The intestinal absorption of n-3 and n-6 polyunsaturated fatty acids: proposed mechanisms from studies using human Caco-2 cells.
… on the specific parameters that influence the transport characteristics of the Caco-2 cells and the accessibility of the fatty acids to the absorption site. The results presented in the first part of this study, show that only early passage Caco-2 cells cultured for 15 day post-confluence in 5 …
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